Acidic Microenvironment Determines Antibiotic Susceptibility and Biofilm Formation of Pseudomonas aeruginosa

Pseudomonas aeruginosa is the most prevalent bacterial species that contribute to cystic fibrosis (CF) respiratory failure. The impaired function of CF transmembrane conductance regulator leads to abnormal epithelial Cl–/HCO3– transport and acidification of airway surface liquid. However, it remains...

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Autores principales:	Qiao Lin, Joseph M. Pilewski, Y. Peter Di
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	cystic fibrosis - CF Pseudomonas aeruginosa antibiotic resistance bacterial evolution acidic pH Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/b6ff318dc43243eebc8a679288196b9d
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spelling	oai:doaj.org-article:b6ff318dc43243eebc8a679288196b9d2021-11-19T06:39:57ZAcidic Microenvironment Determines Antibiotic Susceptibility and Biofilm Formation of Pseudomonas aeruginosa1664-302X10.3389/fmicb.2021.747834https://doaj.org/article/b6ff318dc43243eebc8a679288196b9d2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.747834/fullhttps://doaj.org/toc/1664-302XPseudomonas aeruginosa is the most prevalent bacterial species that contribute to cystic fibrosis (CF) respiratory failure. The impaired function of CF transmembrane conductance regulator leads to abnormal epithelial Cl–/HCO3– transport and acidification of airway surface liquid. However, it remains unclear why the CF lung is most commonly infected by Pseudomonas aeruginosa versus other pathogens. We carried out studies to investigate if lower pH helps Pseudomonas aeruginosa adapt and thrive in the CF-like acidic lung environment. Our results revealed that Pseudomonas aeruginosa generally forms more biofilm, induces antibiotic resistance faster in acidic conditions, and can be reversed by returning the acidic environment to physiologically neutral conditions. Pseudomonas aeruginosa appears to be highly adaptive to the CF-like acidic pH environment. By studying the effects of an acidic environment on bacterial response, we may provide a new therapeutic option in preventing chronic Pseudomonas aeruginosa infection and colonization.Qiao LinJoseph M. PilewskiY. Peter DiFrontiers Media S.A.articlecystic fibrosis - CFPseudomonas aeruginosaantibiotic resistancebacterial evolutionacidic pHMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cystic fibrosis - CF Pseudomonas aeruginosa antibiotic resistance bacterial evolution acidic pH Microbiology QR1-502
spellingShingle	cystic fibrosis - CF Pseudomonas aeruginosa antibiotic resistance bacterial evolution acidic pH Microbiology QR1-502 Qiao Lin Joseph M. Pilewski Y. Peter Di Acidic Microenvironment Determines Antibiotic Susceptibility and Biofilm Formation of Pseudomonas aeruginosa
description	Pseudomonas aeruginosa is the most prevalent bacterial species that contribute to cystic fibrosis (CF) respiratory failure. The impaired function of CF transmembrane conductance regulator leads to abnormal epithelial Cl–/HCO3– transport and acidification of airway surface liquid. However, it remains unclear why the CF lung is most commonly infected by Pseudomonas aeruginosa versus other pathogens. We carried out studies to investigate if lower pH helps Pseudomonas aeruginosa adapt and thrive in the CF-like acidic lung environment. Our results revealed that Pseudomonas aeruginosa generally forms more biofilm, induces antibiotic resistance faster in acidic conditions, and can be reversed by returning the acidic environment to physiologically neutral conditions. Pseudomonas aeruginosa appears to be highly adaptive to the CF-like acidic pH environment. By studying the effects of an acidic environment on bacterial response, we may provide a new therapeutic option in preventing chronic Pseudomonas aeruginosa infection and colonization.
format	article
author	Qiao Lin Joseph M. Pilewski Y. Peter Di
author_facet	Qiao Lin Joseph M. Pilewski Y. Peter Di
author_sort	Qiao Lin
title	Acidic Microenvironment Determines Antibiotic Susceptibility and Biofilm Formation of Pseudomonas aeruginosa
title_short	Acidic Microenvironment Determines Antibiotic Susceptibility and Biofilm Formation of Pseudomonas aeruginosa
title_full	Acidic Microenvironment Determines Antibiotic Susceptibility and Biofilm Formation of Pseudomonas aeruginosa
title_fullStr	Acidic Microenvironment Determines Antibiotic Susceptibility and Biofilm Formation of Pseudomonas aeruginosa
title_full_unstemmed	Acidic Microenvironment Determines Antibiotic Susceptibility and Biofilm Formation of Pseudomonas aeruginosa
title_sort	acidic microenvironment determines antibiotic susceptibility and biofilm formation of pseudomonas aeruginosa
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/b6ff318dc43243eebc8a679288196b9d
work_keys_str_mv	AT qiaolin acidicmicroenvironmentdeterminesantibioticsusceptibilityandbiofilmformationofpseudomonasaeruginosa AT josephmpilewski acidicmicroenvironmentdeterminesantibioticsusceptibilityandbiofilmformationofpseudomonasaeruginosa AT ypeterdi acidicmicroenvironmentdeterminesantibioticsusceptibilityandbiofilmformationofpseudomonasaeruginosa
_version_	1718420337211736064

Acidic Microenvironment Determines Antibiotic Susceptibility and Biofilm Formation of Pseudomonas aeruginosa

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