Phage ϕC2 Mediates Transduction of Tn<italic toggle="yes">6215</italic>, Encoding Erythromycin Resistance, between <named-content content-type="genus-species">Clostridium difficile</named-content> Strains

ABSTRACT In this work, we show that Clostridium difficile phage ϕC2 transduces erm(B), which confers erythromycin resistance, from a donor to a recipient strain at a frequency of 10−6 per PFU. The transductants were lysogenic for ϕC2 and contained the erm(B) gene in a novel transposon, Tn6215. This...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shan Goh, Haitham Hussain, Barbara J. Chang, Warren Emmett, Thomas V. Riley, Peter Mullany
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2013
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/b70367b2f36a45f1a5af31a3cadcb6c9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b70367b2f36a45f1a5af31a3cadcb6c9
record_format	dspace
spelling	oai:doaj.org-article:b70367b2f36a45f1a5af31a3cadcb6c92021-11-15T15:42:32ZPhage ϕC2 Mediates Transduction of Tn<italic toggle="yes">6215</italic>, Encoding Erythromycin Resistance, between <named-content content-type="genus-species">Clostridium difficile</named-content> Strains10.1128/mBio.00840-132150-7511https://doaj.org/article/b70367b2f36a45f1a5af31a3cadcb6c92013-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00840-13https://doaj.org/toc/2150-7511ABSTRACT In this work, we show that Clostridium difficile phage ϕC2 transduces erm(B), which confers erythromycin resistance, from a donor to a recipient strain at a frequency of 10−6 per PFU. The transductants were lysogenic for ϕC2 and contained the erm(B) gene in a novel transposon, Tn6215. This element is 13,008 bp in length and contains 17 putative open reading frames (ORFs). It could also be transferred at a lower frequency by filter mating. IMPORTANCE Clostridium difficile is a major human pathogen that causes diarrhea that can be persistent and difficult to resolve using antibiotics. C. difficile is potentially zoonotic and has been detected in animals, food, and environmental samples. C. difficile genomes contain large portions of horizontally acquired genetic elements. The conjugative elements have been reasonably well studied, but transduction has not yet been demonstrated. Here, we show for the first time transduction as a mechanism for the transfer of a novel genetic element in C. difficile. Transduction may also be a useful tool for the genetic manipulation of C. difficile.Shan GohHaitham HussainBarbara J. ChangWarren EmmettThomas V. RileyPeter MullanyAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 4, Iss 6 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Shan Goh Haitham Hussain Barbara J. Chang Warren Emmett Thomas V. Riley Peter Mullany Phage ϕC2 Mediates Transduction of Tn<italic toggle="yes">6215</italic>, Encoding Erythromycin Resistance, between <named-content content-type="genus-species">Clostridium difficile</named-content> Strains
description	ABSTRACT In this work, we show that Clostridium difficile phage ϕC2 transduces erm(B), which confers erythromycin resistance, from a donor to a recipient strain at a frequency of 10−6 per PFU. The transductants were lysogenic for ϕC2 and contained the erm(B) gene in a novel transposon, Tn6215. This element is 13,008 bp in length and contains 17 putative open reading frames (ORFs). It could also be transferred at a lower frequency by filter mating. IMPORTANCE Clostridium difficile is a major human pathogen that causes diarrhea that can be persistent and difficult to resolve using antibiotics. C. difficile is potentially zoonotic and has been detected in animals, food, and environmental samples. C. difficile genomes contain large portions of horizontally acquired genetic elements. The conjugative elements have been reasonably well studied, but transduction has not yet been demonstrated. Here, we show for the first time transduction as a mechanism for the transfer of a novel genetic element in C. difficile. Transduction may also be a useful tool for the genetic manipulation of C. difficile.
format	article
author	Shan Goh Haitham Hussain Barbara J. Chang Warren Emmett Thomas V. Riley Peter Mullany
author_facet	Shan Goh Haitham Hussain Barbara J. Chang Warren Emmett Thomas V. Riley Peter Mullany
author_sort	Shan Goh
title	Phage ϕC2 Mediates Transduction of Tn<italic toggle="yes">6215</italic>, Encoding Erythromycin Resistance, between <named-content content-type="genus-species">Clostridium difficile</named-content> Strains
title_short	Phage ϕC2 Mediates Transduction of Tn<italic toggle="yes">6215</italic>, Encoding Erythromycin Resistance, between <named-content content-type="genus-species">Clostridium difficile</named-content> Strains
title_full	Phage ϕC2 Mediates Transduction of Tn<italic toggle="yes">6215</italic>, Encoding Erythromycin Resistance, between <named-content content-type="genus-species">Clostridium difficile</named-content> Strains
title_fullStr	Phage ϕC2 Mediates Transduction of Tn<italic toggle="yes">6215</italic>, Encoding Erythromycin Resistance, between <named-content content-type="genus-species">Clostridium difficile</named-content> Strains
title_full_unstemmed	Phage ϕC2 Mediates Transduction of Tn<italic toggle="yes">6215</italic>, Encoding Erythromycin Resistance, between <named-content content-type="genus-species">Clostridium difficile</named-content> Strains
title_sort	phage ϕc2 mediates transduction of tn<italic toggle="yes">6215</italic>, encoding erythromycin resistance, between <named-content content-type="genus-species">clostridium difficile</named-content> strains
publisher	American Society for Microbiology
publishDate	2013
url	https://doaj.org/article/b70367b2f36a45f1a5af31a3cadcb6c9
work_keys_str_mv	AT shangoh phagephc2mediatestransductionoftnitalictoggleyes6215italicencodingerythromycinresistancebetweennamedcontentcontenttypegenusspeciesclostridiumdifficilenamedcontentstrains AT haithamhussain phagephc2mediatestransductionoftnitalictoggleyes6215italicencodingerythromycinresistancebetweennamedcontentcontenttypegenusspeciesclostridiumdifficilenamedcontentstrains AT barbarajchang phagephc2mediatestransductionoftnitalictoggleyes6215italicencodingerythromycinresistancebetweennamedcontentcontenttypegenusspeciesclostridiumdifficilenamedcontentstrains AT warrenemmett phagephc2mediatestransductionoftnitalictoggleyes6215italicencodingerythromycinresistancebetweennamedcontentcontenttypegenusspeciesclostridiumdifficilenamedcontentstrains AT thomasvriley phagephc2mediatestransductionoftnitalictoggleyes6215italicencodingerythromycinresistancebetweennamedcontentcontenttypegenusspeciesclostridiumdifficilenamedcontentstrains AT petermullany phagephc2mediatestransductionoftnitalictoggleyes6215italicencodingerythromycinresistancebetweennamedcontentcontenttypegenusspeciesclostridiumdifficilenamedcontentstrains
_version_	1718427607312105472

Phage ϕC2 Mediates Transduction of Tn<italic toggle="yes">6215</italic>, Encoding Erythromycin Resistance, between <named-content content-type="genus-species">Clostridium difficile</named-content> Strains

Ejemplares similares