Enhanced genome editing efficiency of CRISPR PLUS: Cas9 chimeric fusion proteins

Abstract Efforts to improve CRISPR-Cas9 genome editing systems for lower off-target effects are mostly at the cost of its robust on-target efficiency. To enhance both accuracy and efficiency, we created chimeric SpyCas9 proteins fused with the 5′-to-3′ exonuclease Recombination J (RecJ) or with GFP...

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Autores principales:	Jongjin Park, Jiyoung Yoon, Daekee Kwon, Mi-Jung Han, Sunmee Choi, Slki Park, Junghyuk Lee, Kiwook Lee, Jaehwan Lee, Seunghee Lee, Kyung-Sun Kang, Sunghwa Choe
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b70942f95a9247b8bb22b7e0f6eacb48
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spelling	oai:doaj.org-article:b70942f95a9247b8bb22b7e0f6eacb482021-12-02T16:27:54ZEnhanced genome editing efficiency of CRISPR PLUS: Cas9 chimeric fusion proteins10.1038/s41598-021-95406-82045-2322https://doaj.org/article/b70942f95a9247b8bb22b7e0f6eacb482021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95406-8https://doaj.org/toc/2045-2322Abstract Efforts to improve CRISPR-Cas9 genome editing systems for lower off-target effects are mostly at the cost of its robust on-target efficiency. To enhance both accuracy and efficiency, we created chimeric SpyCas9 proteins fused with the 5′-to-3′ exonuclease Recombination J (RecJ) or with GFP and demonstrated that transfection of the pre-assembled ribonucleoprotein of the two chimeric proteins into human or plant cells resulted in greater targeted mutagenesis efficiency up to 600% without noticeable increase in off-target effects. Improved activity of the two fusion proteins should enable editing of the previously hard-to-edit genes and thus readily obtaining the cells with designer traits.Jongjin ParkJiyoung YoonDaekee KwonMi-Jung HanSunmee ChoiSlki ParkJunghyuk LeeKiwook LeeJaehwan LeeSeunghee LeeKyung-Sun KangSunghwa ChoeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jongjin Park Jiyoung Yoon Daekee Kwon Mi-Jung Han Sunmee Choi Slki Park Junghyuk Lee Kiwook Lee Jaehwan Lee Seunghee Lee Kyung-Sun Kang Sunghwa Choe Enhanced genome editing efficiency of CRISPR PLUS: Cas9 chimeric fusion proteins
description	Abstract Efforts to improve CRISPR-Cas9 genome editing systems for lower off-target effects are mostly at the cost of its robust on-target efficiency. To enhance both accuracy and efficiency, we created chimeric SpyCas9 proteins fused with the 5′-to-3′ exonuclease Recombination J (RecJ) or with GFP and demonstrated that transfection of the pre-assembled ribonucleoprotein of the two chimeric proteins into human or plant cells resulted in greater targeted mutagenesis efficiency up to 600% without noticeable increase in off-target effects. Improved activity of the two fusion proteins should enable editing of the previously hard-to-edit genes and thus readily obtaining the cells with designer traits.
format	article
author	Jongjin Park Jiyoung Yoon Daekee Kwon Mi-Jung Han Sunmee Choi Slki Park Junghyuk Lee Kiwook Lee Jaehwan Lee Seunghee Lee Kyung-Sun Kang Sunghwa Choe
author_facet	Jongjin Park Jiyoung Yoon Daekee Kwon Mi-Jung Han Sunmee Choi Slki Park Junghyuk Lee Kiwook Lee Jaehwan Lee Seunghee Lee Kyung-Sun Kang Sunghwa Choe
author_sort	Jongjin Park
title	Enhanced genome editing efficiency of CRISPR PLUS: Cas9 chimeric fusion proteins
title_short	Enhanced genome editing efficiency of CRISPR PLUS: Cas9 chimeric fusion proteins
title_full	Enhanced genome editing efficiency of CRISPR PLUS: Cas9 chimeric fusion proteins
title_fullStr	Enhanced genome editing efficiency of CRISPR PLUS: Cas9 chimeric fusion proteins
title_full_unstemmed	Enhanced genome editing efficiency of CRISPR PLUS: Cas9 chimeric fusion proteins
title_sort	enhanced genome editing efficiency of crispr plus: cas9 chimeric fusion proteins
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b70942f95a9247b8bb22b7e0f6eacb48
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Enhanced genome editing efficiency of CRISPR PLUS: Cas9 chimeric fusion proteins

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