Identification of hub genes associated with cognition in the hippocampus of Alzheimer’s Disease
Alzheimer’s Disease (AD) is a neurodegenerative disease featured by cognitive impairment. This bioinformatic analysis was to identify hub genes related to cognitive dysfunction in AD. The gene expression profile GSE48350 in the hippocampus of AD patients aged >70y was obtained from the Gene Expre...
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2021
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oai:doaj.org-article:b70d6b6391dc4090a7a656cca7a3cae42021-11-04T15:51:54ZIdentification of hub genes associated with cognition in the hippocampus of Alzheimer’s Disease2165-59792165-598710.1080/21655979.2021.1999549https://doaj.org/article/b70d6b6391dc4090a7a656cca7a3cae42021-10-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1999549https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Alzheimer’s Disease (AD) is a neurodegenerative disease featured by cognitive impairment. This bioinformatic analysis was to identify hub genes related to cognitive dysfunction in AD. The gene expression profile GSE48350 in the hippocampus of AD patients aged >70y was obtained from the Gene Expression Omnibus (GEO) database. A total of 96 differentially expressed genes (DEGs) were identified, and subjected to Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses; a protein-protein interaction (PPI) network was constructed. The DEGs were enriched in synapse-related changes. A protein cluster was teased out of PPI. Furthermore, the cognition ranked the first among all the terms of biological process (BP). Next, 4 of 10 hub genes enriched in cognition were identified. The function of these genes was validated using APP/PS1 mice. Cognitive performance was validated by Morris Water Maze (MWM), and gene expression by RT-qPCR, Cholecystokinin (CCK), Tachykinin precursor 1 (TAC1), Calbindin 1 (CALB1) were downregulated in the hippocampus. These genes can provide new directions in the research the molecular mechanism of AD.Yu-Jia LiuTian-Tian LiuLin-Hao JiangQian LiuZheng-Liang MaTian-Jiao XiaXiao-Ping GuTaylor & Francis Grouparticlealzheimer’s diseasebioinformatic analysiscognitiongse48350BiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021) |
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alzheimer’s disease bioinformatic analysis cognition gse48350 Biotechnology TP248.13-248.65 |
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alzheimer’s disease bioinformatic analysis cognition gse48350 Biotechnology TP248.13-248.65 Yu-Jia Liu Tian-Tian Liu Lin-Hao Jiang Qian Liu Zheng-Liang Ma Tian-Jiao Xia Xiao-Ping Gu Identification of hub genes associated with cognition in the hippocampus of Alzheimer’s Disease |
| description |
Alzheimer’s Disease (AD) is a neurodegenerative disease featured by cognitive impairment. This bioinformatic analysis was to identify hub genes related to cognitive dysfunction in AD. The gene expression profile GSE48350 in the hippocampus of AD patients aged >70y was obtained from the Gene Expression Omnibus (GEO) database. A total of 96 differentially expressed genes (DEGs) were identified, and subjected to Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses; a protein-protein interaction (PPI) network was constructed. The DEGs were enriched in synapse-related changes. A protein cluster was teased out of PPI. Furthermore, the cognition ranked the first among all the terms of biological process (BP). Next, 4 of 10 hub genes enriched in cognition were identified. The function of these genes was validated using APP/PS1 mice. Cognitive performance was validated by Morris Water Maze (MWM), and gene expression by RT-qPCR, Cholecystokinin (CCK), Tachykinin precursor 1 (TAC1), Calbindin 1 (CALB1) were downregulated in the hippocampus. These genes can provide new directions in the research the molecular mechanism of AD. |
| format |
article |
| author |
Yu-Jia Liu Tian-Tian Liu Lin-Hao Jiang Qian Liu Zheng-Liang Ma Tian-Jiao Xia Xiao-Ping Gu |
| author_facet |
Yu-Jia Liu Tian-Tian Liu Lin-Hao Jiang Qian Liu Zheng-Liang Ma Tian-Jiao Xia Xiao-Ping Gu |
| author_sort |
Yu-Jia Liu |
| title |
Identification of hub genes associated with cognition in the hippocampus of Alzheimer’s Disease |
| title_short |
Identification of hub genes associated with cognition in the hippocampus of Alzheimer’s Disease |
| title_full |
Identification of hub genes associated with cognition in the hippocampus of Alzheimer’s Disease |
| title_fullStr |
Identification of hub genes associated with cognition in the hippocampus of Alzheimer’s Disease |
| title_full_unstemmed |
Identification of hub genes associated with cognition in the hippocampus of Alzheimer’s Disease |
| title_sort |
identification of hub genes associated with cognition in the hippocampus of alzheimer’s disease |
| publisher |
Taylor & Francis Group |
| publishDate |
2021 |
| url |
https://doaj.org/article/b70d6b6391dc4090a7a656cca7a3cae4 |
| work_keys_str_mv |
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| _version_ |
1718444735736053760 |