Resistance to a rhabdovirus (VHSV) in rainbow trout: identification of a major QTL related to innate mechanisms.

Health control is a major issue in animal breeding and a better knowledge of the genetic bases of resistance to diseases is needed in farm animals including fish. The detection of quantitative trait loci (QTL) will help uncovering the genetic architecture of important traits and understanding the me...

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Autores principales:	Eloi R Verrier, Michel Dorson, Stéphane Mauger, Corinne Torhy, Céline Ciobotaru, Caroline Hervet, Nicolas Dechamp, Carine Genet, Pierre Boudinot, Edwige Quillet
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b7114b502efc459b90980e76b87ffa16
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spelling	oai:doaj.org-article:b7114b502efc459b90980e76b87ffa162021-11-18T07:58:54ZResistance to a rhabdovirus (VHSV) in rainbow trout: identification of a major QTL related to innate mechanisms.1932-620310.1371/journal.pone.0055302https://doaj.org/article/b7114b502efc459b90980e76b87ffa162013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23390526/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Health control is a major issue in animal breeding and a better knowledge of the genetic bases of resistance to diseases is needed in farm animals including fish. The detection of quantitative trait loci (QTL) will help uncovering the genetic architecture of important traits and understanding the mechanisms involved in resistance to pathogens. We report here the detection of QTL for resistance to Viral Haemorrhagic Septicaemia Virus (VHSV), a major threat for European aquaculture industry. Two induced mitogynogenetic doubled haploid F2 rainbow trout (Oncorhynchus mykiss) families were used. These families combined the genome of susceptible and resistant F0 breeders and contained only fully homozygous individuals. For phenotyping, fish survival after an immersion challenge with the virus was recorded, as well as in vitro virus replication on fin explants. A bidirectional selective genotyping strategy identified seven QTL associated to survival. One of those QTL was significant at the genome-wide level and largely explained both survival and viral replication in fin explants in the different families of the design (up to 65% and 49% of phenotypic variance explained respectively). These results evidence the key role of innate defence in resistance to the virus and pave the way for the identification of the gene(s) responsible for resistance. The identification of a major QTL also opens appealing perspectives for selective breeding of fish with improved resistance.Eloi R VerrierMichel DorsonStéphane MaugerCorinne TorhyCéline CiobotaruCaroline HervetNicolas DechampCarine GenetPierre BoudinotEdwige QuilletPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 2, p e55302 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Eloi R Verrier Michel Dorson Stéphane Mauger Corinne Torhy Céline Ciobotaru Caroline Hervet Nicolas Dechamp Carine Genet Pierre Boudinot Edwige Quillet Resistance to a rhabdovirus (VHSV) in rainbow trout: identification of a major QTL related to innate mechanisms.
description	Health control is a major issue in animal breeding and a better knowledge of the genetic bases of resistance to diseases is needed in farm animals including fish. The detection of quantitative trait loci (QTL) will help uncovering the genetic architecture of important traits and understanding the mechanisms involved in resistance to pathogens. We report here the detection of QTL for resistance to Viral Haemorrhagic Septicaemia Virus (VHSV), a major threat for European aquaculture industry. Two induced mitogynogenetic doubled haploid F2 rainbow trout (Oncorhynchus mykiss) families were used. These families combined the genome of susceptible and resistant F0 breeders and contained only fully homozygous individuals. For phenotyping, fish survival after an immersion challenge with the virus was recorded, as well as in vitro virus replication on fin explants. A bidirectional selective genotyping strategy identified seven QTL associated to survival. One of those QTL was significant at the genome-wide level and largely explained both survival and viral replication in fin explants in the different families of the design (up to 65% and 49% of phenotypic variance explained respectively). These results evidence the key role of innate defence in resistance to the virus and pave the way for the identification of the gene(s) responsible for resistance. The identification of a major QTL also opens appealing perspectives for selective breeding of fish with improved resistance.
format	article
author	Eloi R Verrier Michel Dorson Stéphane Mauger Corinne Torhy Céline Ciobotaru Caroline Hervet Nicolas Dechamp Carine Genet Pierre Boudinot Edwige Quillet
author_facet	Eloi R Verrier Michel Dorson Stéphane Mauger Corinne Torhy Céline Ciobotaru Caroline Hervet Nicolas Dechamp Carine Genet Pierre Boudinot Edwige Quillet
author_sort	Eloi R Verrier
title	Resistance to a rhabdovirus (VHSV) in rainbow trout: identification of a major QTL related to innate mechanisms.
title_short	Resistance to a rhabdovirus (VHSV) in rainbow trout: identification of a major QTL related to innate mechanisms.
title_full	Resistance to a rhabdovirus (VHSV) in rainbow trout: identification of a major QTL related to innate mechanisms.
title_fullStr	Resistance to a rhabdovirus (VHSV) in rainbow trout: identification of a major QTL related to innate mechanisms.
title_full_unstemmed	Resistance to a rhabdovirus (VHSV) in rainbow trout: identification of a major QTL related to innate mechanisms.
title_sort	resistance to a rhabdovirus (vhsv) in rainbow trout: identification of a major qtl related to innate mechanisms.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/b7114b502efc459b90980e76b87ffa16
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Resistance to a rhabdovirus (VHSV) in rainbow trout: identification of a major QTL related to innate mechanisms.

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