Pressure Perturbation Studies of Noncanonical Viral Nucleic Acid Structures

G-quadruplexes are noncanonical structures formed by guanine-rich sequences of the genome. They are found in crucial loci of the human genome, they take part in the regulation of important processes like cell proliferation and cell death. Much less is known about the subjects of this work, the viral...

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Autores principales: Judit Somkuti, Orsolya Réka Molnár, Anna Grád, László Smeller
Formato: article
Lenguaje:EN
Publicado: MDPI AG 2021
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DNA
Acceso en línea:https://doaj.org/article/b711b83caacb4b1eb0eda7cecc833ff1
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spelling oai:doaj.org-article:b711b83caacb4b1eb0eda7cecc833ff12021-11-25T16:47:41ZPressure Perturbation Studies of Noncanonical Viral Nucleic Acid Structures10.3390/biology101111732079-7737https://doaj.org/article/b711b83caacb4b1eb0eda7cecc833ff12021-11-01T00:00:00Zhttps://www.mdpi.com/2079-7737/10/11/1173https://doaj.org/toc/2079-7737G-quadruplexes are noncanonical structures formed by guanine-rich sequences of the genome. They are found in crucial loci of the human genome, they take part in the regulation of important processes like cell proliferation and cell death. Much less is known about the subjects of this work, the viral G-quadruplexes. We have chosen three potentially G-quadruplex-forming sequences of hepatitis B. We measured the stability and the thermodynamic parameters of these quadruplexes. We also investigated the potential stabilization of these G-quadruplexes by binding a special ligand that was originally developed for cancer therapy. Fluorescence and infrared spectroscopic measurements were performed over wide temperature and pressure ranges. Our experiments indicate the small unfolding volume change of all three oligos. We found a difference between the unfolding of the 2-quartet and the 3-quartet G-quadruplexes. All three G-quadruplexes were stabilized by TMPyP4, which is a cationic porphyrin developed for stabilizing the human telomere.Judit SomkutiOrsolya Réka MolnárAnna GrádLászló SmellerMDPI AGarticleG-quadruplexhepatitis BDNAoligoFRETFTIRBiology (General)QH301-705.5ENBiology, Vol 10, Iss 1173, p 1173 (2021)
institution DOAJ
collection DOAJ
language EN
topic G-quadruplex
hepatitis B
DNA
oligo
FRET
FTIR
Biology (General)
QH301-705.5
spellingShingle G-quadruplex
hepatitis B
DNA
oligo
FRET
FTIR
Biology (General)
QH301-705.5
Judit Somkuti
Orsolya Réka Molnár
Anna Grád
László Smeller
Pressure Perturbation Studies of Noncanonical Viral Nucleic Acid Structures
description G-quadruplexes are noncanonical structures formed by guanine-rich sequences of the genome. They are found in crucial loci of the human genome, they take part in the regulation of important processes like cell proliferation and cell death. Much less is known about the subjects of this work, the viral G-quadruplexes. We have chosen three potentially G-quadruplex-forming sequences of hepatitis B. We measured the stability and the thermodynamic parameters of these quadruplexes. We also investigated the potential stabilization of these G-quadruplexes by binding a special ligand that was originally developed for cancer therapy. Fluorescence and infrared spectroscopic measurements were performed over wide temperature and pressure ranges. Our experiments indicate the small unfolding volume change of all three oligos. We found a difference between the unfolding of the 2-quartet and the 3-quartet G-quadruplexes. All three G-quadruplexes were stabilized by TMPyP4, which is a cationic porphyrin developed for stabilizing the human telomere.
format article
author Judit Somkuti
Orsolya Réka Molnár
Anna Grád
László Smeller
author_facet Judit Somkuti
Orsolya Réka Molnár
Anna Grád
László Smeller
author_sort Judit Somkuti
title Pressure Perturbation Studies of Noncanonical Viral Nucleic Acid Structures
title_short Pressure Perturbation Studies of Noncanonical Viral Nucleic Acid Structures
title_full Pressure Perturbation Studies of Noncanonical Viral Nucleic Acid Structures
title_fullStr Pressure Perturbation Studies of Noncanonical Viral Nucleic Acid Structures
title_full_unstemmed Pressure Perturbation Studies of Noncanonical Viral Nucleic Acid Structures
title_sort pressure perturbation studies of noncanonical viral nucleic acid structures
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/b711b83caacb4b1eb0eda7cecc833ff1
work_keys_str_mv AT juditsomkuti pressureperturbationstudiesofnoncanonicalviralnucleicacidstructures
AT orsolyarekamolnar pressureperturbationstudiesofnoncanonicalviralnucleicacidstructures
AT annagrad pressureperturbationstudiesofnoncanonicalviralnucleicacidstructures
AT laszlosmeller pressureperturbationstudiesofnoncanonicalviralnucleicacidstructures
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