Pressure Perturbation Studies of Noncanonical Viral Nucleic Acid Structures
G-quadruplexes are noncanonical structures formed by guanine-rich sequences of the genome. They are found in crucial loci of the human genome, they take part in the regulation of important processes like cell proliferation and cell death. Much less is known about the subjects of this work, the viral...
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2021
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oai:doaj.org-article:b711b83caacb4b1eb0eda7cecc833ff12021-11-25T16:47:41ZPressure Perturbation Studies of Noncanonical Viral Nucleic Acid Structures10.3390/biology101111732079-7737https://doaj.org/article/b711b83caacb4b1eb0eda7cecc833ff12021-11-01T00:00:00Zhttps://www.mdpi.com/2079-7737/10/11/1173https://doaj.org/toc/2079-7737G-quadruplexes are noncanonical structures formed by guanine-rich sequences of the genome. They are found in crucial loci of the human genome, they take part in the regulation of important processes like cell proliferation and cell death. Much less is known about the subjects of this work, the viral G-quadruplexes. We have chosen three potentially G-quadruplex-forming sequences of hepatitis B. We measured the stability and the thermodynamic parameters of these quadruplexes. We also investigated the potential stabilization of these G-quadruplexes by binding a special ligand that was originally developed for cancer therapy. Fluorescence and infrared spectroscopic measurements were performed over wide temperature and pressure ranges. Our experiments indicate the small unfolding volume change of all three oligos. We found a difference between the unfolding of the 2-quartet and the 3-quartet G-quadruplexes. All three G-quadruplexes were stabilized by TMPyP4, which is a cationic porphyrin developed for stabilizing the human telomere.Judit SomkutiOrsolya Réka MolnárAnna GrádLászló SmellerMDPI AGarticleG-quadruplexhepatitis BDNAoligoFRETFTIRBiology (General)QH301-705.5ENBiology, Vol 10, Iss 1173, p 1173 (2021) |
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G-quadruplex hepatitis B DNA oligo FRET FTIR Biology (General) QH301-705.5 |
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G-quadruplex hepatitis B DNA oligo FRET FTIR Biology (General) QH301-705.5 Judit Somkuti Orsolya Réka Molnár Anna Grád László Smeller Pressure Perturbation Studies of Noncanonical Viral Nucleic Acid Structures |
description |
G-quadruplexes are noncanonical structures formed by guanine-rich sequences of the genome. They are found in crucial loci of the human genome, they take part in the regulation of important processes like cell proliferation and cell death. Much less is known about the subjects of this work, the viral G-quadruplexes. We have chosen three potentially G-quadruplex-forming sequences of hepatitis B. We measured the stability and the thermodynamic parameters of these quadruplexes. We also investigated the potential stabilization of these G-quadruplexes by binding a special ligand that was originally developed for cancer therapy. Fluorescence and infrared spectroscopic measurements were performed over wide temperature and pressure ranges. Our experiments indicate the small unfolding volume change of all three oligos. We found a difference between the unfolding of the 2-quartet and the 3-quartet G-quadruplexes. All three G-quadruplexes were stabilized by TMPyP4, which is a cationic porphyrin developed for stabilizing the human telomere. |
format |
article |
author |
Judit Somkuti Orsolya Réka Molnár Anna Grád László Smeller |
author_facet |
Judit Somkuti Orsolya Réka Molnár Anna Grád László Smeller |
author_sort |
Judit Somkuti |
title |
Pressure Perturbation Studies of Noncanonical Viral Nucleic Acid Structures |
title_short |
Pressure Perturbation Studies of Noncanonical Viral Nucleic Acid Structures |
title_full |
Pressure Perturbation Studies of Noncanonical Viral Nucleic Acid Structures |
title_fullStr |
Pressure Perturbation Studies of Noncanonical Viral Nucleic Acid Structures |
title_full_unstemmed |
Pressure Perturbation Studies of Noncanonical Viral Nucleic Acid Structures |
title_sort |
pressure perturbation studies of noncanonical viral nucleic acid structures |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doaj.org/article/b711b83caacb4b1eb0eda7cecc833ff1 |
work_keys_str_mv |
AT juditsomkuti pressureperturbationstudiesofnoncanonicalviralnucleicacidstructures AT orsolyarekamolnar pressureperturbationstudiesofnoncanonicalviralnucleicacidstructures AT annagrad pressureperturbationstudiesofnoncanonicalviralnucleicacidstructures AT laszlosmeller pressureperturbationstudiesofnoncanonicalviralnucleicacidstructures |
_version_ |
1718412978153324544 |