Comparative transcriptomic profiling of susceptible and resistant cultivars of pigeonpea demonstrates early molecular responses during Fusarium udum infection

Abstract Vascular wilt caused by Fusarium udum Butler is the most important disease of pigeonpea throughout the world. F. udum isolate MTCC 2204 (M1) inoculated pigeonpea plants of susceptible (ICP 2376) and resistant (ICP 8863) cultivars were taken at invasion stage of pathogenesis process for tran...

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Autores principales:	Arnab Purohit, Sanatan Ghosh, Shreeparna Ganguly, Madan Singh Negi, Shashi Bhushan Tripathi, Rituparna Kundu Chaudhuri, Dipankar Chakraborti
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b71818bd94b044c1a8ffbc063ee04fdc
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spelling	oai:doaj.org-article:b71818bd94b044c1a8ffbc063ee04fdc2021-11-21T12:19:06ZComparative transcriptomic profiling of susceptible and resistant cultivars of pigeonpea demonstrates early molecular responses during Fusarium udum infection10.1038/s41598-021-01587-72045-2322https://doaj.org/article/b71818bd94b044c1a8ffbc063ee04fdc2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01587-7https://doaj.org/toc/2045-2322Abstract Vascular wilt caused by Fusarium udum Butler is the most important disease of pigeonpea throughout the world. F. udum isolate MTCC 2204 (M1) inoculated pigeonpea plants of susceptible (ICP 2376) and resistant (ICP 8863) cultivars were taken at invasion stage of pathogenesis process for transcriptomic profiling to understand defense signaling reactions that interplay at early stage of this plant–pathogen encounter. Differential transcriptomic profiles were generated through cDNA-AFLP from M1 inoculated resistant and susceptible pigeonpea root tissues. Twenty five percent of transcript derived fragments (TDFs) were found to be pathogen induced. Among them 73 TDFs were re-amplified and sequenced. Homology search of the TDFs in available databases and thorough study of scientific literature identified several pathways, which could play crucial role in defense responses of the F. udum inoculated resistant plants. Some of the defense responsive pathways identified to be active during this interaction are, jasmonic acid and salicylic acid mediated defense responses, cell wall remodeling, vascular development and pattering, abscisic acid mediated responses, effector triggered immunity, and reactive oxygen species mediated signaling. This study identified important wilt responsive regulatory pathways in pigeonpea which will be helpful for further exploration of these resistant components for pigeonpea improvement.Arnab PurohitSanatan GhoshShreeparna GangulyMadan Singh NegiShashi Bhushan TripathiRituparna Kundu ChaudhuriDipankar ChakrabortiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Arnab Purohit Sanatan Ghosh Shreeparna Ganguly Madan Singh Negi Shashi Bhushan Tripathi Rituparna Kundu Chaudhuri Dipankar Chakraborti Comparative transcriptomic profiling of susceptible and resistant cultivars of pigeonpea demonstrates early molecular responses during Fusarium udum infection
description	Abstract Vascular wilt caused by Fusarium udum Butler is the most important disease of pigeonpea throughout the world. F. udum isolate MTCC 2204 (M1) inoculated pigeonpea plants of susceptible (ICP 2376) and resistant (ICP 8863) cultivars were taken at invasion stage of pathogenesis process for transcriptomic profiling to understand defense signaling reactions that interplay at early stage of this plant–pathogen encounter. Differential transcriptomic profiles were generated through cDNA-AFLP from M1 inoculated resistant and susceptible pigeonpea root tissues. Twenty five percent of transcript derived fragments (TDFs) were found to be pathogen induced. Among them 73 TDFs were re-amplified and sequenced. Homology search of the TDFs in available databases and thorough study of scientific literature identified several pathways, which could play crucial role in defense responses of the F. udum inoculated resistant plants. Some of the defense responsive pathways identified to be active during this interaction are, jasmonic acid and salicylic acid mediated defense responses, cell wall remodeling, vascular development and pattering, abscisic acid mediated responses, effector triggered immunity, and reactive oxygen species mediated signaling. This study identified important wilt responsive regulatory pathways in pigeonpea which will be helpful for further exploration of these resistant components for pigeonpea improvement.
format	article
author	Arnab Purohit Sanatan Ghosh Shreeparna Ganguly Madan Singh Negi Shashi Bhushan Tripathi Rituparna Kundu Chaudhuri Dipankar Chakraborti
author_facet	Arnab Purohit Sanatan Ghosh Shreeparna Ganguly Madan Singh Negi Shashi Bhushan Tripathi Rituparna Kundu Chaudhuri Dipankar Chakraborti
author_sort	Arnab Purohit
title	Comparative transcriptomic profiling of susceptible and resistant cultivars of pigeonpea demonstrates early molecular responses during Fusarium udum infection
title_short	Comparative transcriptomic profiling of susceptible and resistant cultivars of pigeonpea demonstrates early molecular responses during Fusarium udum infection
title_full	Comparative transcriptomic profiling of susceptible and resistant cultivars of pigeonpea demonstrates early molecular responses during Fusarium udum infection
title_fullStr	Comparative transcriptomic profiling of susceptible and resistant cultivars of pigeonpea demonstrates early molecular responses during Fusarium udum infection
title_full_unstemmed	Comparative transcriptomic profiling of susceptible and resistant cultivars of pigeonpea demonstrates early molecular responses during Fusarium udum infection
title_sort	comparative transcriptomic profiling of susceptible and resistant cultivars of pigeonpea demonstrates early molecular responses during fusarium udum infection
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b71818bd94b044c1a8ffbc063ee04fdc
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Comparative transcriptomic profiling of susceptible and resistant cultivars of pigeonpea demonstrates early molecular responses during Fusarium udum infection

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