Cell-free synthetic biochemistry upgrading of ethanol to 1,3 butanediol

Abstract It is now possible to efficiently fix flue gas CO/CO2 into ethanol using acetogens, thereby making carbon negative ethanol. While the ethanol could be burned as a fuel, returning the CO2 to the atmosphere, it might also be possible to use the fixed carbon in more diverse chemicals, thereby...

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Autores principales:	Hongjiang Liu, James U. Bowie
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b7187d1dcdff4a3e914f8a1b4e8e652a
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spelling	oai:doaj.org-article:b7187d1dcdff4a3e914f8a1b4e8e652a2021-12-02T14:49:34ZCell-free synthetic biochemistry upgrading of ethanol to 1,3 butanediol10.1038/s41598-021-88899-w2045-2322https://doaj.org/article/b7187d1dcdff4a3e914f8a1b4e8e652a2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88899-whttps://doaj.org/toc/2045-2322Abstract It is now possible to efficiently fix flue gas CO/CO2 into ethanol using acetogens, thereby making carbon negative ethanol. While the ethanol could be burned as a fuel, returning the CO2 to the atmosphere, it might also be possible to use the fixed carbon in more diverse chemicals, thereby keeping it fixed. Here we describe a simple synthetic biochemistry approach for converting carbon negative ethanol into the synthetic building block chemical 1,3 butanediol (1,3-BDO). The pathway completely conserves carbon from ethanol and can ultimately be powered electrochemically via formate oxidation. Our proof-of-principle system reached a maximum productivity of 0.16 g/L/h and, with replenishment of feedstock and enzymes, achieved a titer of 7.7 g/L. We identify a number of elements that can be addressed in future work to improve both cell-free and cell-based production of 1,3-BDO.Hongjiang LiuJames U. BowieNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hongjiang Liu James U. Bowie Cell-free synthetic biochemistry upgrading of ethanol to 1,3 butanediol
description	Abstract It is now possible to efficiently fix flue gas CO/CO2 into ethanol using acetogens, thereby making carbon negative ethanol. While the ethanol could be burned as a fuel, returning the CO2 to the atmosphere, it might also be possible to use the fixed carbon in more diverse chemicals, thereby keeping it fixed. Here we describe a simple synthetic biochemistry approach for converting carbon negative ethanol into the synthetic building block chemical 1,3 butanediol (1,3-BDO). The pathway completely conserves carbon from ethanol and can ultimately be powered electrochemically via formate oxidation. Our proof-of-principle system reached a maximum productivity of 0.16 g/L/h and, with replenishment of feedstock and enzymes, achieved a titer of 7.7 g/L. We identify a number of elements that can be addressed in future work to improve both cell-free and cell-based production of 1,3-BDO.
format	article
author	Hongjiang Liu James U. Bowie
author_facet	Hongjiang Liu James U. Bowie
author_sort	Hongjiang Liu
title	Cell-free synthetic biochemistry upgrading of ethanol to 1,3 butanediol
title_short	Cell-free synthetic biochemistry upgrading of ethanol to 1,3 butanediol
title_full	Cell-free synthetic biochemistry upgrading of ethanol to 1,3 butanediol
title_fullStr	Cell-free synthetic biochemistry upgrading of ethanol to 1,3 butanediol
title_full_unstemmed	Cell-free synthetic biochemistry upgrading of ethanol to 1,3 butanediol
title_sort	cell-free synthetic biochemistry upgrading of ethanol to 1,3 butanediol
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b7187d1dcdff4a3e914f8a1b4e8e652a
work_keys_str_mv	AT hongjiangliu cellfreesyntheticbiochemistryupgradingofethanolto13butanediol AT jamesubowie cellfreesyntheticbiochemistryupgradingofethanolto13butanediol
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Cell-free synthetic biochemistry upgrading of ethanol to 1,3 butanediol

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