The Survival of Pear Dormant Buds at Ultra-Low Temperatures

Currently, there is a varietal diversity decline in pear orchards of the Czech Republic. Thus, the safe storage of their gene pool collections is becoming increasingly important. Therefore, the ultra-low temperature survival after two-step cryopreservation treatment of dormant buds was tested for a...

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Autores principales:	Alois Bilavcik, Milos Faltus, Jiri Zamecnik
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cryopreservation dormant buds low-temperature resistance pear tree Botany QK1-989
Acceso en línea:	https://doaj.org/article/b727241bb1f64099be1eb2e13d9e43fe
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spelling	oai:doaj.org-article:b727241bb1f64099be1eb2e13d9e43fe2021-11-25T18:47:08ZThe Survival of Pear Dormant Buds at Ultra-Low Temperatures10.3390/plants101125022223-7747https://doaj.org/article/b727241bb1f64099be1eb2e13d9e43fe2021-11-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2502https://doaj.org/toc/2223-7747Currently, there is a varietal diversity decline in pear orchards of the Czech Republic. Thus, the safe storage of their gene pool collections is becoming increasingly important. Therefore, the ultra-low temperature survival after two-step cryopreservation treatment of dormant buds was tested for a safe and rapid way to conserve pear germplasm in a broader range of varieties. The following varieties crucial for cultivation in the Czech Republic were tested; ‘Amfora’, ‘Beurré Hardy’, ‘Bosc’, ‘Clapp’s Favourite’, ‘Conference’, ‘Dicolor’, ‘Erika’, ‘Lucas’, ‘Williams’ and ‘Williams Red’. In 2011 and 2012, dormant pear buds were dehydrated to 40.1% and 36.0% water content, respectively, before cryopreservation. The average regeneration of the dormant pear buds after cryopreservation by the two-step cryoprotocol in 2011 and 2012 was 54.3% and 16.1%, respectively. The mentioned cryopreservation procedure is suitable for the safe storage of dormant buds in most tested pear varieties.Alois BilavcikMilos FaltusJiri ZamecnikMDPI AGarticlecryopreservationdormant budslow-temperature resistancepear treeBotanyQK1-989ENPlants, Vol 10, Iss 2502, p 2502 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cryopreservation dormant buds low-temperature resistance pear tree Botany QK1-989
spellingShingle	cryopreservation dormant buds low-temperature resistance pear tree Botany QK1-989 Alois Bilavcik Milos Faltus Jiri Zamecnik The Survival of Pear Dormant Buds at Ultra-Low Temperatures
description	Currently, there is a varietal diversity decline in pear orchards of the Czech Republic. Thus, the safe storage of their gene pool collections is becoming increasingly important. Therefore, the ultra-low temperature survival after two-step cryopreservation treatment of dormant buds was tested for a safe and rapid way to conserve pear germplasm in a broader range of varieties. The following varieties crucial for cultivation in the Czech Republic were tested; ‘Amfora’, ‘Beurré Hardy’, ‘Bosc’, ‘Clapp’s Favourite’, ‘Conference’, ‘Dicolor’, ‘Erika’, ‘Lucas’, ‘Williams’ and ‘Williams Red’. In 2011 and 2012, dormant pear buds were dehydrated to 40.1% and 36.0% water content, respectively, before cryopreservation. The average regeneration of the dormant pear buds after cryopreservation by the two-step cryoprotocol in 2011 and 2012 was 54.3% and 16.1%, respectively. The mentioned cryopreservation procedure is suitable for the safe storage of dormant buds in most tested pear varieties.
format	article
author	Alois Bilavcik Milos Faltus Jiri Zamecnik
author_facet	Alois Bilavcik Milos Faltus Jiri Zamecnik
author_sort	Alois Bilavcik
title	The Survival of Pear Dormant Buds at Ultra-Low Temperatures
title_short	The Survival of Pear Dormant Buds at Ultra-Low Temperatures
title_full	The Survival of Pear Dormant Buds at Ultra-Low Temperatures
title_fullStr	The Survival of Pear Dormant Buds at Ultra-Low Temperatures
title_full_unstemmed	The Survival of Pear Dormant Buds at Ultra-Low Temperatures
title_sort	survival of pear dormant buds at ultra-low temperatures
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b727241bb1f64099be1eb2e13d9e43fe
work_keys_str_mv	AT aloisbilavcik thesurvivalofpeardormantbudsatultralowtemperatures AT milosfaltus thesurvivalofpeardormantbudsatultralowtemperatures AT jirizamecnik thesurvivalofpeardormantbudsatultralowtemperatures AT aloisbilavcik survivalofpeardormantbudsatultralowtemperatures AT milosfaltus survivalofpeardormantbudsatultralowtemperatures AT jirizamecnik survivalofpeardormantbudsatultralowtemperatures
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The Survival of Pear Dormant Buds at Ultra-Low Temperatures

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