Dissemination of the transmissible quinolone-resistance gene qnrS1 by IncX plasmids in Nigeria.

The plasmid-encoded quinolone resistance gene qnrS1 was recently found to be commonly associated with ciprofloxacin resistance in Nigeria. We mapped the qnrS1 gene from an Escherichia coli isolate obtained in Nigeria to a 43.5 Kb IncX2 plasmid. The plasmid, pEBG1, was sufficient to confer ciprofloxa...

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Autores principales:	Eric T Sumrall, Elizabeth B Gallo, Aaron Oladipo Aboderin, Adebayo Lamikanra, Iruka N Okeke
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b7379cf48acc455dacd75b4e1de5ba41
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spelling	oai:doaj.org-article:b7379cf48acc455dacd75b4e1de5ba412021-11-25T05:55:24ZDissemination of the transmissible quinolone-resistance gene qnrS1 by IncX plasmids in Nigeria.1932-620310.1371/journal.pone.0110279https://doaj.org/article/b7379cf48acc455dacd75b4e1de5ba412014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0110279https://doaj.org/toc/1932-6203The plasmid-encoded quinolone resistance gene qnrS1 was recently found to be commonly associated with ciprofloxacin resistance in Nigeria. We mapped the qnrS1 gene from an Escherichia coli isolate obtained in Nigeria to a 43.5 Kb IncX2 plasmid. The plasmid, pEBG1, was sufficient to confer ciprofloxacin non-susceptibility, as well as tetracycline and trimethoprim resistance, on E. coli K-12. Deletion analysis confirmed that qnrS1 accounted for all the ciprofloxacin non-suceptibility conferred by pEBG1 and tetracycline and trimethoprim resistance could be attributed to tetAR and dfrA14 genes respectively. While it contained a complete IncX conjugation system, pEBG1 was not self-transmissible likely due to an IS3 element inserted between the pilX5 and pilX6 genes. The plasmid was however efficiently mobilizable. pEBG1 was most similar to another qnrS1-bearing IncX2 plasmid from Nigeria, but both plasmids acquired qnrS1 independently and differ in their content of other resistance genes. Screening qnrS1-positive isolates from other individuals in Nigeria revealed that they carried neither pEBG1 nor pNGX2-QnrS1 but that IncX plasmids were prevalent. This study demonstrates that the IncX backbone is a flexible platform that has contributed to qnrS1 dissemination in Nigeria.Eric T SumrallElizabeth B GalloAaron Oladipo AboderinAdebayo LamikanraIruka N OkekePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 10, p e110279 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Eric T Sumrall Elizabeth B Gallo Aaron Oladipo Aboderin Adebayo Lamikanra Iruka N Okeke Dissemination of the transmissible quinolone-resistance gene qnrS1 by IncX plasmids in Nigeria.
description	The plasmid-encoded quinolone resistance gene qnrS1 was recently found to be commonly associated with ciprofloxacin resistance in Nigeria. We mapped the qnrS1 gene from an Escherichia coli isolate obtained in Nigeria to a 43.5 Kb IncX2 plasmid. The plasmid, pEBG1, was sufficient to confer ciprofloxacin non-susceptibility, as well as tetracycline and trimethoprim resistance, on E. coli K-12. Deletion analysis confirmed that qnrS1 accounted for all the ciprofloxacin non-suceptibility conferred by pEBG1 and tetracycline and trimethoprim resistance could be attributed to tetAR and dfrA14 genes respectively. While it contained a complete IncX conjugation system, pEBG1 was not self-transmissible likely due to an IS3 element inserted between the pilX5 and pilX6 genes. The plasmid was however efficiently mobilizable. pEBG1 was most similar to another qnrS1-bearing IncX2 plasmid from Nigeria, but both plasmids acquired qnrS1 independently and differ in their content of other resistance genes. Screening qnrS1-positive isolates from other individuals in Nigeria revealed that they carried neither pEBG1 nor pNGX2-QnrS1 but that IncX plasmids were prevalent. This study demonstrates that the IncX backbone is a flexible platform that has contributed to qnrS1 dissemination in Nigeria.
format	article
author	Eric T Sumrall Elizabeth B Gallo Aaron Oladipo Aboderin Adebayo Lamikanra Iruka N Okeke
author_facet	Eric T Sumrall Elizabeth B Gallo Aaron Oladipo Aboderin Adebayo Lamikanra Iruka N Okeke
author_sort	Eric T Sumrall
title	Dissemination of the transmissible quinolone-resistance gene qnrS1 by IncX plasmids in Nigeria.
title_short	Dissemination of the transmissible quinolone-resistance gene qnrS1 by IncX plasmids in Nigeria.
title_full	Dissemination of the transmissible quinolone-resistance gene qnrS1 by IncX plasmids in Nigeria.
title_fullStr	Dissemination of the transmissible quinolone-resistance gene qnrS1 by IncX plasmids in Nigeria.
title_full_unstemmed	Dissemination of the transmissible quinolone-resistance gene qnrS1 by IncX plasmids in Nigeria.
title_sort	dissemination of the transmissible quinolone-resistance gene qnrs1 by incx plasmids in nigeria.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/b7379cf48acc455dacd75b4e1de5ba41
work_keys_str_mv	AT erictsumrall disseminationofthetransmissiblequinoloneresistancegeneqnrs1byincxplasmidsinnigeria AT elizabethbgallo disseminationofthetransmissiblequinoloneresistancegeneqnrs1byincxplasmidsinnigeria AT aaronoladipoaboderin disseminationofthetransmissiblequinoloneresistancegeneqnrs1byincxplasmidsinnigeria AT adebayolamikanra disseminationofthetransmissiblequinoloneresistancegeneqnrs1byincxplasmidsinnigeria AT irukanokeke disseminationofthetransmissiblequinoloneresistancegeneqnrs1byincxplasmidsinnigeria
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Dissemination of the transmissible quinolone-resistance gene qnrS1 by IncX plasmids in Nigeria.

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