Evaluation of Vasorlaxant Effect of Achillea Wilhelmsii Hydroalcholic Extract on Isolated Aorta in Rat

BACKGROUND AND OBJECTIVE: Achillea is well known herb in traditional medicine which has anticonvulsant and tonic effects, however it is used to treat tachycardia and angina pectoris. Some reports of antihypertensive and hypotensive effects of Achillea wilhelmsii are published. This study aimed to i...

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Autores principales:	F Harandizadeh, SM Hosseini, M Behnam Rasouli, S Niazmand
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2012
Materias:	achillea wilhelmsii aorta endothelium vasorelaxant Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/b73ae7a4cd4140ffb13535bd895ec8cf
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spelling	oai:doaj.org-article:b73ae7a4cd4140ffb13535bd895ec8cf2021-11-10T08:53:02ZEvaluation of Vasorlaxant Effect of Achillea Wilhelmsii Hydroalcholic Extract on Isolated Aorta in Rat1561-41072251-7170https://doaj.org/article/b73ae7a4cd4140ffb13535bd895ec8cf2012-03-01T00:00:00Zhttp://jbums.org/article-1-4045-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Achillea is well known herb in traditional medicine which has anticonvulsant and tonic effects, however it is used to treat tachycardia and angina pectoris. Some reports of antihypertensive and hypotensive effects of Achillea wilhelmsii are published. This study aimed to investigate the vasorelaxant effect of Achillea wilhelmsii hydroalcholic extract on isolated aorta in rat.METHODS: It was an interventional study in which 42 male Wistar rats (weighed 200-250 g) were divided randomly into 6 groups. In groups 1 and 2 the effect of the extract (1, 5 and 25 mg/ml) on contracted aorta by phenylephrine in intact and denuded endothelium were investigated. In groups 3 and 4 the effect of the extract on contracted aorta by chloride potassium in intact and denuded endothelium were investigated. In groups 5 and 6 the effect of the extract (5 mg/ml) on contracted aorta by phenylephrine and chloride potassium in the presence of cumulative calcium concentrations were evaluated by isolated tissue setup. FINDINGS: In intact aorta contracted by phenylephrine, the extract relaxed 8%, 47% (p<0.001) and 45% (p<0.001), respectively, at concentrations 1, 5 and 25 mg/ml. In denuded endothelium aorta contracted by phenylephrine the extract relaxed 16% (p<0.05), 56% (p<0.001) and 54% (p<0.001), respectively, at concentrations 1, 5 and 25 mg/ml. In intact aorta contracted by chloride potassium the extract relaxed 18% (p<0.01), 57% (p<0.001) and 57% (p<0.001), respectively, at concentrations 1, 5 and 25 mg/ml. In denuded endothelium aorta contracted by chloride potassium the extract relaxed 2.5%, 47% (p<0.001) and 54.5% (p<0.001), respectively, at concentrations 1, 5 and 25 mg/ml. The extract (5 mg/ml) relaxed 7% (p<0.001), 12% (p<0.001), 22% (p<0.001) and 31.5% (p<0.001). The extract (5 mg/ml) relaxed 5.5% (p<0.01), 9.3% (p<0.001), 19% (p<0.001) and 74% (p<0.001) the contracted aorta by chloride potassium in the presence of cumulative calcium concentrations (from 10-5 to 10-2 respectively).CONCLUSION: The extract showed endothelium-independent vasorelaxant effect. The relaxation mainly was mediated by inhibition of voltage- and receptor-dependent Ca2+ channels in vascular smooth muscle cells.F HarandizadehSM HosseiniM Behnam RasouliS NiazmandBabol University of Medical Sciencesarticleachillea wilhelmsiiaortaendotheliumvasorelaxantMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 14, Iss 2, Pp 39-46 (2012)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	achillea wilhelmsii aorta endothelium vasorelaxant Medicine R Medicine (General) R5-920
spellingShingle	achillea wilhelmsii aorta endothelium vasorelaxant Medicine R Medicine (General) R5-920 F Harandizadeh SM Hosseini M Behnam Rasouli S Niazmand Evaluation of Vasorlaxant Effect of Achillea Wilhelmsii Hydroalcholic Extract on Isolated Aorta in Rat
description	BACKGROUND AND OBJECTIVE: Achillea is well known herb in traditional medicine which has anticonvulsant and tonic effects, however it is used to treat tachycardia and angina pectoris. Some reports of antihypertensive and hypotensive effects of Achillea wilhelmsii are published. This study aimed to investigate the vasorelaxant effect of Achillea wilhelmsii hydroalcholic extract on isolated aorta in rat.METHODS: It was an interventional study in which 42 male Wistar rats (weighed 200-250 g) were divided randomly into 6 groups. In groups 1 and 2 the effect of the extract (1, 5 and 25 mg/ml) on contracted aorta by phenylephrine in intact and denuded endothelium were investigated. In groups 3 and 4 the effect of the extract on contracted aorta by chloride potassium in intact and denuded endothelium were investigated. In groups 5 and 6 the effect of the extract (5 mg/ml) on contracted aorta by phenylephrine and chloride potassium in the presence of cumulative calcium concentrations were evaluated by isolated tissue setup. FINDINGS: In intact aorta contracted by phenylephrine, the extract relaxed 8%, 47% (p<0.001) and 45% (p<0.001), respectively, at concentrations 1, 5 and 25 mg/ml. In denuded endothelium aorta contracted by phenylephrine the extract relaxed 16% (p<0.05), 56% (p<0.001) and 54% (p<0.001), respectively, at concentrations 1, 5 and 25 mg/ml. In intact aorta contracted by chloride potassium the extract relaxed 18% (p<0.01), 57% (p<0.001) and 57% (p<0.001), respectively, at concentrations 1, 5 and 25 mg/ml. In denuded endothelium aorta contracted by chloride potassium the extract relaxed 2.5%, 47% (p<0.001) and 54.5% (p<0.001), respectively, at concentrations 1, 5 and 25 mg/ml. The extract (5 mg/ml) relaxed 7% (p<0.001), 12% (p<0.001), 22% (p<0.001) and 31.5% (p<0.001). The extract (5 mg/ml) relaxed 5.5% (p<0.01), 9.3% (p<0.001), 19% (p<0.001) and 74% (p<0.001) the contracted aorta by chloride potassium in the presence of cumulative calcium concentrations (from 10-5 to 10-2 respectively).CONCLUSION: The extract showed endothelium-independent vasorelaxant effect. The relaxation mainly was mediated by inhibition of voltage- and receptor-dependent Ca2+ channels in vascular smooth muscle cells.
format	article
author	F Harandizadeh SM Hosseini M Behnam Rasouli S Niazmand
author_facet	F Harandizadeh SM Hosseini M Behnam Rasouli S Niazmand
author_sort	F Harandizadeh
title	Evaluation of Vasorlaxant Effect of Achillea Wilhelmsii Hydroalcholic Extract on Isolated Aorta in Rat
title_short	Evaluation of Vasorlaxant Effect of Achillea Wilhelmsii Hydroalcholic Extract on Isolated Aorta in Rat
title_full	Evaluation of Vasorlaxant Effect of Achillea Wilhelmsii Hydroalcholic Extract on Isolated Aorta in Rat
title_fullStr	Evaluation of Vasorlaxant Effect of Achillea Wilhelmsii Hydroalcholic Extract on Isolated Aorta in Rat
title_full_unstemmed	Evaluation of Vasorlaxant Effect of Achillea Wilhelmsii Hydroalcholic Extract on Isolated Aorta in Rat
title_sort	evaluation of vasorlaxant effect of achillea wilhelmsii hydroalcholic extract on isolated aorta in rat
publisher	Babol University of Medical Sciences
publishDate	2012
url	https://doaj.org/article/b73ae7a4cd4140ffb13535bd895ec8cf
work_keys_str_mv	AT fharandizadeh evaluationofvasorlaxanteffectofachilleawilhelmsiihydroalcholicextractonisolatedaortainrat AT smhosseini evaluationofvasorlaxanteffectofachilleawilhelmsiihydroalcholicextractonisolatedaortainrat AT mbehnamrasouli evaluationofvasorlaxanteffectofachilleawilhelmsiihydroalcholicextractonisolatedaortainrat AT sniazmand evaluationofvasorlaxanteffectofachilleawilhelmsiihydroalcholicextractonisolatedaortainrat
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Evaluation of Vasorlaxant Effect of Achillea Wilhelmsii Hydroalcholic Extract on Isolated Aorta in Rat

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