Physiological and transcriptomic analyses characterized high temperature stress response mechanisms in Sorbus pohuashanensis

Abstract Sorbus pohuashanensis (Hance) Hedl. is a Chinese native alpine tree species, but the problem of introducing S. pohuashanensis to low altitude areas has not been solved. In this study, we aimed to explore the molecular regulatory network of S. pohuashanensis in response to high-temperature s...

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Autores principales:	Xin Pei, Yan Zhang, Lingyi Zhu, Dongxue Zhao, Yizeng Lu, Jian Zheng
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b7471622af7e4d88b366028398bb4d07
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spelling	oai:doaj.org-article:b7471622af7e4d88b366028398bb4d072021-12-02T15:43:17ZPhysiological and transcriptomic analyses characterized high temperature stress response mechanisms in Sorbus pohuashanensis10.1038/s41598-021-89418-72045-2322https://doaj.org/article/b7471622af7e4d88b366028398bb4d072021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89418-7https://doaj.org/toc/2045-2322Abstract Sorbus pohuashanensis (Hance) Hedl. is a Chinese native alpine tree species, but the problem of introducing S. pohuashanensis to low altitude areas has not been solved. In this study, we aimed to explore the molecular regulatory network of S. pohuashanensis in response to high-temperature stress using RNA-Sequencing technology and physiological and biochemical determination. Based on transcriptomic data, we obtained 1221 genes (752 up-regulated and 469 down-regulated) that were differentially expressed during 8 h 43℃ treatment and candidate genes were related to calcium signaling pathway, plant hormone signal transduction, heat shock factors, chaperones, ubiquitin mediated proteolysis, cell wall modification, ROS scavenging enzymes, detoxification and energy metabolism. The analysis of high temperature response at the physiological level and biochemical level were performed. The chlorophyll fluorescence parameters of leaf cells decreased, the content of osmotic regulators increased, and the activity of ROS scavenging enzymes decreased. The molecular regulatory network of S. pohuashanensis in response to high-temperature stress was preliminarily revealed in this study, which provides fundamental information improving introducing methods and discovering heat-tolerant genes involved in high-temperature stress in this species and provides a reference for other plants of the genus Sorbus.Xin PeiYan ZhangLingyi ZhuDongxue ZhaoYizeng LuJian ZhengNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-21 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xin Pei Yan Zhang Lingyi Zhu Dongxue Zhao Yizeng Lu Jian Zheng Physiological and transcriptomic analyses characterized high temperature stress response mechanisms in Sorbus pohuashanensis
description	Abstract Sorbus pohuashanensis (Hance) Hedl. is a Chinese native alpine tree species, but the problem of introducing S. pohuashanensis to low altitude areas has not been solved. In this study, we aimed to explore the molecular regulatory network of S. pohuashanensis in response to high-temperature stress using RNA-Sequencing technology and physiological and biochemical determination. Based on transcriptomic data, we obtained 1221 genes (752 up-regulated and 469 down-regulated) that were differentially expressed during 8 h 43℃ treatment and candidate genes were related to calcium signaling pathway, plant hormone signal transduction, heat shock factors, chaperones, ubiquitin mediated proteolysis, cell wall modification, ROS scavenging enzymes, detoxification and energy metabolism. The analysis of high temperature response at the physiological level and biochemical level were performed. The chlorophyll fluorescence parameters of leaf cells decreased, the content of osmotic regulators increased, and the activity of ROS scavenging enzymes decreased. The molecular regulatory network of S. pohuashanensis in response to high-temperature stress was preliminarily revealed in this study, which provides fundamental information improving introducing methods and discovering heat-tolerant genes involved in high-temperature stress in this species and provides a reference for other plants of the genus Sorbus.
format	article
author	Xin Pei Yan Zhang Lingyi Zhu Dongxue Zhao Yizeng Lu Jian Zheng
author_facet	Xin Pei Yan Zhang Lingyi Zhu Dongxue Zhao Yizeng Lu Jian Zheng
author_sort	Xin Pei
title	Physiological and transcriptomic analyses characterized high temperature stress response mechanisms in Sorbus pohuashanensis
title_short	Physiological and transcriptomic analyses characterized high temperature stress response mechanisms in Sorbus pohuashanensis
title_full	Physiological and transcriptomic analyses characterized high temperature stress response mechanisms in Sorbus pohuashanensis
title_fullStr	Physiological and transcriptomic analyses characterized high temperature stress response mechanisms in Sorbus pohuashanensis
title_full_unstemmed	Physiological and transcriptomic analyses characterized high temperature stress response mechanisms in Sorbus pohuashanensis
title_sort	physiological and transcriptomic analyses characterized high temperature stress response mechanisms in sorbus pohuashanensis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b7471622af7e4d88b366028398bb4d07
work_keys_str_mv	AT xinpei physiologicalandtranscriptomicanalysescharacterizedhightemperaturestressresponsemechanismsinsorbuspohuashanensis AT yanzhang physiologicalandtranscriptomicanalysescharacterizedhightemperaturestressresponsemechanismsinsorbuspohuashanensis AT lingyizhu physiologicalandtranscriptomicanalysescharacterizedhightemperaturestressresponsemechanismsinsorbuspohuashanensis AT dongxuezhao physiologicalandtranscriptomicanalysescharacterizedhightemperaturestressresponsemechanismsinsorbuspohuashanensis AT yizenglu physiologicalandtranscriptomicanalysescharacterizedhightemperaturestressresponsemechanismsinsorbuspohuashanensis AT jianzheng physiologicalandtranscriptomicanalysescharacterizedhightemperaturestressresponsemechanismsinsorbuspohuashanensis
_version_	1718385831305019392

Physiological and transcriptomic analyses characterized high temperature stress response mechanisms in Sorbus pohuashanensis

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