Increased hippocampal excitability in miR-324-null mice

Abstract MicroRNAs are non-coding RNAs that act to downregulate the expression of target genes by translational repression and degradation of messenger RNA molecules. Individual microRNAs have the ability to specifically target a wide array of gene transcripts, therefore allowing each microRNA to pl...

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Autores principales:	Dan J. Hayman, Tamara Modebadze, Sarah Charlton, Kat Cheung, Jamie Soul, Hua Lin, Yao Hao, Colin G. Miles, Dimitra Tsompani, Robert M. Jackson, Michael D. Briggs, Katarzyna A. Piróg, Ian M. Clark, Matt J. Barter, Gavin J. Clowry, Fiona E. N. LeBeau, David A. Young
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b74a1a1210eb465eb49f8ba80fb52c10
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spelling	oai:doaj.org-article:b74a1a1210eb465eb49f8ba80fb52c102021-12-02T15:53:10ZIncreased hippocampal excitability in miR-324-null mice10.1038/s41598-021-89874-12045-2322https://doaj.org/article/b74a1a1210eb465eb49f8ba80fb52c102021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89874-1https://doaj.org/toc/2045-2322Abstract MicroRNAs are non-coding RNAs that act to downregulate the expression of target genes by translational repression and degradation of messenger RNA molecules. Individual microRNAs have the ability to specifically target a wide array of gene transcripts, therefore allowing each microRNA to play key roles in multiple biological pathways. miR-324 is a microRNA predicted to target thousands of RNA transcripts and is expressed far more highly in the brain than in any other tissue, suggesting that it may play a role in one or multiple neurological pathways. Here we present data from the first global miR-324-null mice, in which increased excitability and interictal discharges were identified in vitro in the hippocampus. RNA sequencing was used to identify differentially expressed genes in miR-324-null mice which may contribute to this increased hippocampal excitability, and 3′UTR luciferase assays and western blotting revealed that two of these, Suox and Cd300lf, are novel direct targets of miR-324. Characterisation of microRNAs that produce an effect on neurological activity, such as miR-324, and identification of the pathways they regulate will allow a better understanding of the processes involved in normal neurological function and in turn may present novel pharmaceutical targets in treating neurological disease.Dan J. HaymanTamara ModebadzeSarah CharltonKat CheungJamie SoulHua LinYao HaoColin G. MilesDimitra TsompaniRobert M. JacksonMichael D. BriggsKatarzyna A. PirógIan M. ClarkMatt J. BarterGavin J. ClowryFiona E. N. LeBeauDavid A. YoungNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dan J. Hayman Tamara Modebadze Sarah Charlton Kat Cheung Jamie Soul Hua Lin Yao Hao Colin G. Miles Dimitra Tsompani Robert M. Jackson Michael D. Briggs Katarzyna A. Piróg Ian M. Clark Matt J. Barter Gavin J. Clowry Fiona E. N. LeBeau David A. Young Increased hippocampal excitability in miR-324-null mice
description	Abstract MicroRNAs are non-coding RNAs that act to downregulate the expression of target genes by translational repression and degradation of messenger RNA molecules. Individual microRNAs have the ability to specifically target a wide array of gene transcripts, therefore allowing each microRNA to play key roles in multiple biological pathways. miR-324 is a microRNA predicted to target thousands of RNA transcripts and is expressed far more highly in the brain than in any other tissue, suggesting that it may play a role in one or multiple neurological pathways. Here we present data from the first global miR-324-null mice, in which increased excitability and interictal discharges were identified in vitro in the hippocampus. RNA sequencing was used to identify differentially expressed genes in miR-324-null mice which may contribute to this increased hippocampal excitability, and 3′UTR luciferase assays and western blotting revealed that two of these, Suox and Cd300lf, are novel direct targets of miR-324. Characterisation of microRNAs that produce an effect on neurological activity, such as miR-324, and identification of the pathways they regulate will allow a better understanding of the processes involved in normal neurological function and in turn may present novel pharmaceutical targets in treating neurological disease.
format	article
author	Dan J. Hayman Tamara Modebadze Sarah Charlton Kat Cheung Jamie Soul Hua Lin Yao Hao Colin G. Miles Dimitra Tsompani Robert M. Jackson Michael D. Briggs Katarzyna A. Piróg Ian M. Clark Matt J. Barter Gavin J. Clowry Fiona E. N. LeBeau David A. Young
author_facet	Dan J. Hayman Tamara Modebadze Sarah Charlton Kat Cheung Jamie Soul Hua Lin Yao Hao Colin G. Miles Dimitra Tsompani Robert M. Jackson Michael D. Briggs Katarzyna A. Piróg Ian M. Clark Matt J. Barter Gavin J. Clowry Fiona E. N. LeBeau David A. Young
author_sort	Dan J. Hayman
title	Increased hippocampal excitability in miR-324-null mice
title_short	Increased hippocampal excitability in miR-324-null mice
title_full	Increased hippocampal excitability in miR-324-null mice
title_fullStr	Increased hippocampal excitability in miR-324-null mice
title_full_unstemmed	Increased hippocampal excitability in miR-324-null mice
title_sort	increased hippocampal excitability in mir-324-null mice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b74a1a1210eb465eb49f8ba80fb52c10
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Increased hippocampal excitability in miR-324-null mice

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