Integrated Bioinformatics Analysis Identifies Heat Shock Factor 2 as a Prognostic Biomarker Associated With Immune Cell Infiltration in Hepatocellular Carcinoma

Hepatocellular carcinoma (HCC) is one of the most common malignancies and ranks as the second leading cause of cancer-related mortality worldwide. Heat shock factor 2 (HSF2) is a transcription factor that plays a critical role in development, particularly corticogenesis and spermatogenesis. However,...

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Autores principales:	Yumei Fan, Jiajie Hou, Xiaopeng Liu, Bihui Han, Yanxiu Meng, Bing Liu, Fei Chen, Yanan Shang, Pengxiu Cao, Ke Tan
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	HSF2 hepatocellular carcinoma prognostic biomarker immune infiltration immunotherapy Genetics QH426-470
Acceso en línea:	https://doaj.org/article/b74cd8ef73c247b788ca7cc6e7fb813d
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spelling	oai:doaj.org-article:b74cd8ef73c247b788ca7cc6e7fb813d2021-12-01T18:32:59ZIntegrated Bioinformatics Analysis Identifies Heat Shock Factor 2 as a Prognostic Biomarker Associated With Immune Cell Infiltration in Hepatocellular Carcinoma1664-802110.3389/fgene.2021.668516https://doaj.org/article/b74cd8ef73c247b788ca7cc6e7fb813d2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fgene.2021.668516/fullhttps://doaj.org/toc/1664-8021Hepatocellular carcinoma (HCC) is one of the most common malignancies and ranks as the second leading cause of cancer-related mortality worldwide. Heat shock factor 2 (HSF2) is a transcription factor that plays a critical role in development, particularly corticogenesis and spermatogenesis. However, studies examining the expression and prognostic value of HSF2 and its association with tumor-infiltrating immune cells in HCC are still rare. In the present study, we found that HSF2 expression was significantly upregulated in HCC tissues compared with normal liver tissues using the TCGA, ICGC, GEO, UALCAN, HCCDB and HPA databases. High HSF2 expression was associated with shorter survival of patients with HCC. Cox regression analyses and nomogram were used to evaluate the association of HSF2 expression with the prognosis of patients with HCC. Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis and gene set enrichment analysis (GSEA) revealed that HSF2 was associated with various signaling pathways, including the immune response. Notably, HSF2 expression was significantly correlated with the infiltration levels of different immune cells using the TIMER database and CIBERSORT algorithm. HSF2 expression also displayed a significant correlation with multiple immune marker sets in HCC tissues. Knockdown of HSF2 significantly inhibited the proliferation, migration, invasion and colony formation ability of HCC cells. In summary, we explored the clinical significance of HSF2 and provided a therapeutic basis for the early diagnosis, prognostic judgment, and immunotherapy of HCC.Yumei FanJiajie HouXiaopeng LiuXiaopeng LiuBihui HanYanxiu MengBing LiuFei ChenYanan ShangPengxiu CaoKe TanFrontiers Media S.A.articleHSF2hepatocellular carcinomaprognostic biomarkerimmune infiltrationimmunotherapyGeneticsQH426-470ENFrontiers in Genetics, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	HSF2 hepatocellular carcinoma prognostic biomarker immune infiltration immunotherapy Genetics QH426-470
spellingShingle	HSF2 hepatocellular carcinoma prognostic biomarker immune infiltration immunotherapy Genetics QH426-470 Yumei Fan Jiajie Hou Xiaopeng Liu Xiaopeng Liu Bihui Han Yanxiu Meng Bing Liu Fei Chen Yanan Shang Pengxiu Cao Ke Tan Integrated Bioinformatics Analysis Identifies Heat Shock Factor 2 as a Prognostic Biomarker Associated With Immune Cell Infiltration in Hepatocellular Carcinoma
description	Hepatocellular carcinoma (HCC) is one of the most common malignancies and ranks as the second leading cause of cancer-related mortality worldwide. Heat shock factor 2 (HSF2) is a transcription factor that plays a critical role in development, particularly corticogenesis and spermatogenesis. However, studies examining the expression and prognostic value of HSF2 and its association with tumor-infiltrating immune cells in HCC are still rare. In the present study, we found that HSF2 expression was significantly upregulated in HCC tissues compared with normal liver tissues using the TCGA, ICGC, GEO, UALCAN, HCCDB and HPA databases. High HSF2 expression was associated with shorter survival of patients with HCC. Cox regression analyses and nomogram were used to evaluate the association of HSF2 expression with the prognosis of patients with HCC. Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis and gene set enrichment analysis (GSEA) revealed that HSF2 was associated with various signaling pathways, including the immune response. Notably, HSF2 expression was significantly correlated with the infiltration levels of different immune cells using the TIMER database and CIBERSORT algorithm. HSF2 expression also displayed a significant correlation with multiple immune marker sets in HCC tissues. Knockdown of HSF2 significantly inhibited the proliferation, migration, invasion and colony formation ability of HCC cells. In summary, we explored the clinical significance of HSF2 and provided a therapeutic basis for the early diagnosis, prognostic judgment, and immunotherapy of HCC.
format	article
author	Yumei Fan Jiajie Hou Xiaopeng Liu Xiaopeng Liu Bihui Han Yanxiu Meng Bing Liu Fei Chen Yanan Shang Pengxiu Cao Ke Tan
author_facet	Yumei Fan Jiajie Hou Xiaopeng Liu Xiaopeng Liu Bihui Han Yanxiu Meng Bing Liu Fei Chen Yanan Shang Pengxiu Cao Ke Tan
author_sort	Yumei Fan
title	Integrated Bioinformatics Analysis Identifies Heat Shock Factor 2 as a Prognostic Biomarker Associated With Immune Cell Infiltration in Hepatocellular Carcinoma
title_short	Integrated Bioinformatics Analysis Identifies Heat Shock Factor 2 as a Prognostic Biomarker Associated With Immune Cell Infiltration in Hepatocellular Carcinoma
title_full	Integrated Bioinformatics Analysis Identifies Heat Shock Factor 2 as a Prognostic Biomarker Associated With Immune Cell Infiltration in Hepatocellular Carcinoma
title_fullStr	Integrated Bioinformatics Analysis Identifies Heat Shock Factor 2 as a Prognostic Biomarker Associated With Immune Cell Infiltration in Hepatocellular Carcinoma
title_full_unstemmed	Integrated Bioinformatics Analysis Identifies Heat Shock Factor 2 as a Prognostic Biomarker Associated With Immune Cell Infiltration in Hepatocellular Carcinoma
title_sort	integrated bioinformatics analysis identifies heat shock factor 2 as a prognostic biomarker associated with immune cell infiltration in hepatocellular carcinoma
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/b74cd8ef73c247b788ca7cc6e7fb813d
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Integrated Bioinformatics Analysis Identifies Heat Shock Factor 2 as a Prognostic Biomarker Associated With Immune Cell Infiltration in Hepatocellular Carcinoma

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