Comparative transcriptomic analysis of gonadal development and renewal in the ovoviviparous black rockfish (Sebastes schlegelii)

Abstract Background The black rockfish (Sebastes schlegelii) has an ovoviviparous reproductive pattern and long-term sperm storage, resulting in asynchronous gonadal development between the sexes. However, the comprehensive understanding of gonadal development in black rockfish has not yet been achi...

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Autores principales:	Jianshuang Li, Likang Lyu, Haishen Wen, Yun Li, Xiaojie Wang, Ying Zhang, Yijia Yao, Xin Qi
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Black rockfish Transcriptomic Gonadal development RNA-seq Biotechnology TP248.13-248.65 Genetics QH426-470
Acceso en línea:	https://doaj.org/article/b74fdee75b0949f3a4e745c738e6c6c0
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spelling	oai:doaj.org-article:b74fdee75b0949f3a4e745c738e6c6c02021-12-05T12:17:22ZComparative transcriptomic analysis of gonadal development and renewal in the ovoviviparous black rockfish (Sebastes schlegelii)10.1186/s12864-021-08169-x1471-2164https://doaj.org/article/b74fdee75b0949f3a4e745c738e6c6c02021-12-01T00:00:00Zhttps://doi.org/10.1186/s12864-021-08169-xhttps://doaj.org/toc/1471-2164Abstract Background The black rockfish (Sebastes schlegelii) has an ovoviviparous reproductive pattern and long-term sperm storage, resulting in asynchronous gonadal development between the sexes. However, the comprehensive understanding of gonadal development in black rockfish has not yet been achieved. Here, we studied gonadal development and germ cell renewal using histology and RNA-seq. Results In this study, RNA-seq was performed on testes and ovaries to characterize key pathways and genes that are active during development and gamete maturation in black rockfish. Differentially expressed genes (DEGs) were identified and annotated in 4 comparisons (F_III vs. F_IV, F_IV vs. F_V, M_III vs. M_IV and M_IV vs. M_V). Based on analysis of DEGs enriched in the testis, 11 and 14 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were mapped to the M_III vs. M_IV group and the M_IV vs. M_V group, respectively. DEGs in ovarian development were also classified into 10 groups according to their biological functions. The expression patterns of the selected genes determined by qPCR were significantly correlated with the RNA-Seq results, supporting the reliability and accuracy of the RNA-Seq analysis. E2 levels showed down regulation from previtellogenesis to mature stage in female and T level showed down regulation from spermatogenesis to regressed stage in the male. Conclusions The categories “intercellular interaction and cytoskeleton”, “molecule amplification” and “repair in the cell cycle” were revealed to be crucial in testis development and spermatogenesis, as was the biosynthesis of a series of metabolites. Our results provide comprehensive insight into black rockfish gonadal development and provide a basis for further study of reproductive physiology and molecular biology in ovoviviparity teleosts.Jianshuang LiLikang LyuHaishen WenYun LiXiaojie WangYing ZhangYijia YaoXin QiBMCarticleBlack rockfishTranscriptomicGonadal developmentRNA-seqBiotechnologyTP248.13-248.65GeneticsQH426-470ENBMC Genomics, Vol 22, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Black rockfish Transcriptomic Gonadal development RNA-seq Biotechnology TP248.13-248.65 Genetics QH426-470
spellingShingle	Black rockfish Transcriptomic Gonadal development RNA-seq Biotechnology TP248.13-248.65 Genetics QH426-470 Jianshuang Li Likang Lyu Haishen Wen Yun Li Xiaojie Wang Ying Zhang Yijia Yao Xin Qi Comparative transcriptomic analysis of gonadal development and renewal in the ovoviviparous black rockfish (Sebastes schlegelii)
description	Abstract Background The black rockfish (Sebastes schlegelii) has an ovoviviparous reproductive pattern and long-term sperm storage, resulting in asynchronous gonadal development between the sexes. However, the comprehensive understanding of gonadal development in black rockfish has not yet been achieved. Here, we studied gonadal development and germ cell renewal using histology and RNA-seq. Results In this study, RNA-seq was performed on testes and ovaries to characterize key pathways and genes that are active during development and gamete maturation in black rockfish. Differentially expressed genes (DEGs) were identified and annotated in 4 comparisons (F_III vs. F_IV, F_IV vs. F_V, M_III vs. M_IV and M_IV vs. M_V). Based on analysis of DEGs enriched in the testis, 11 and 14 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were mapped to the M_III vs. M_IV group and the M_IV vs. M_V group, respectively. DEGs in ovarian development were also classified into 10 groups according to their biological functions. The expression patterns of the selected genes determined by qPCR were significantly correlated with the RNA-Seq results, supporting the reliability and accuracy of the RNA-Seq analysis. E2 levels showed down regulation from previtellogenesis to mature stage in female and T level showed down regulation from spermatogenesis to regressed stage in the male. Conclusions The categories “intercellular interaction and cytoskeleton”, “molecule amplification” and “repair in the cell cycle” were revealed to be crucial in testis development and spermatogenesis, as was the biosynthesis of a series of metabolites. Our results provide comprehensive insight into black rockfish gonadal development and provide a basis for further study of reproductive physiology and molecular biology in ovoviviparity teleosts.
format	article
author	Jianshuang Li Likang Lyu Haishen Wen Yun Li Xiaojie Wang Ying Zhang Yijia Yao Xin Qi
author_facet	Jianshuang Li Likang Lyu Haishen Wen Yun Li Xiaojie Wang Ying Zhang Yijia Yao Xin Qi
author_sort	Jianshuang Li
title	Comparative transcriptomic analysis of gonadal development and renewal in the ovoviviparous black rockfish (Sebastes schlegelii)
title_short	Comparative transcriptomic analysis of gonadal development and renewal in the ovoviviparous black rockfish (Sebastes schlegelii)
title_full	Comparative transcriptomic analysis of gonadal development and renewal in the ovoviviparous black rockfish (Sebastes schlegelii)
title_fullStr	Comparative transcriptomic analysis of gonadal development and renewal in the ovoviviparous black rockfish (Sebastes schlegelii)
title_full_unstemmed	Comparative transcriptomic analysis of gonadal development and renewal in the ovoviviparous black rockfish (Sebastes schlegelii)
title_sort	comparative transcriptomic analysis of gonadal development and renewal in the ovoviviparous black rockfish (sebastes schlegelii)
publisher	BMC
publishDate	2021
url	https://doaj.org/article/b74fdee75b0949f3a4e745c738e6c6c0
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Comparative transcriptomic analysis of gonadal development and renewal in the ovoviviparous black rockfish (Sebastes schlegelii)

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