MNSs blood group glycophorin variants in Taiwan: a genotype-serotype correlation study of 'Mi(a)' and St(a) with report of two new alleles for St(a).

<h4>Background</h4>Glycophorin variants of the MNSs blood group are important in Taiwan. For more than 20 years, screening for the most frequent irregular antibody, anti-''Mi(a)', has been conducted by using 'Mi(a)'(+) RBCs, with a significant success. However, t...

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Autores principales:	Tai-Di Chen, Ding-Ping Chen, Wei-Ting Wang, Chien-Feng Sun
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b751ec6b6887497ab5fd7f0d11a7099b
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spelling	oai:doaj.org-article:b751ec6b6887497ab5fd7f0d11a7099b2021-11-18T08:18:11ZMNSs blood group glycophorin variants in Taiwan: a genotype-serotype correlation study of 'Mi(a)' and St(a) with report of two new alleles for St(a).1932-620310.1371/journal.pone.0098166https://doaj.org/article/b751ec6b6887497ab5fd7f0d11a7099b2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24858913/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Glycophorin variants of the MNSs blood group are important in Taiwan. For more than 20 years, screening for the most frequent irregular antibody, anti-''Mi(a)', has been conducted by using 'Mi(a)'(+) RBCs, with a significant success. However, the sensitivity and the specificity of this screening strategy have never been validated, and the true incidences of different glycophorin variants in Taiwan have been in controversy. Also, the significance of another less frequent and usually separately reported variant, St(a), has never been evaluated.<h4>Methodology/principal findings</h4>We ran a population-based screening (from unselected patients in our hospital) for MNSs blood group glycophorin variants by PCR-sequencing method. GP.Mur (Mil.III) was confirmed by sequence from 57 out of 1027 samples (5.6%), and there was no other Miltenberger subtype glycophorin variant found. Glycophorin variant St(a) was found from 35 out of 1027 samples (3.4%). In contrast to anti-'Mi(a)', which is the most frequently identified irregular antibody in Taiwan, the prevalence of anti-St(a) was only 0.13% as determined by serologic method. In addition, two new alleles for St(a) were found and reported.<h4>Conclusion/significance</h4>We confirm the long-standing assumption that GP.Mur is the only prevalent Miltenberger subtype in Taiwan. The current anti-'Mi(a)' screening method used in Taiwan, although neither sensitive nor specific, is still a suitable practice. Although St(a) antigen has a high prevalence in Taiwan, routine screening for anti-St(a) is not warranted based on current evidence.Tai-Di ChenDing-Ping ChenWei-Ting WangChien-Feng SunPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 5, p e98166 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tai-Di Chen Ding-Ping Chen Wei-Ting Wang Chien-Feng Sun MNSs blood group glycophorin variants in Taiwan: a genotype-serotype correlation study of 'Mi(a)' and St(a) with report of two new alleles for St(a).
description	<h4>Background</h4>Glycophorin variants of the MNSs blood group are important in Taiwan. For more than 20 years, screening for the most frequent irregular antibody, anti-''Mi(a)', has been conducted by using 'Mi(a)'(+) RBCs, with a significant success. However, the sensitivity and the specificity of this screening strategy have never been validated, and the true incidences of different glycophorin variants in Taiwan have been in controversy. Also, the significance of another less frequent and usually separately reported variant, St(a), has never been evaluated.<h4>Methodology/principal findings</h4>We ran a population-based screening (from unselected patients in our hospital) for MNSs blood group glycophorin variants by PCR-sequencing method. GP.Mur (Mil.III) was confirmed by sequence from 57 out of 1027 samples (5.6%), and there was no other Miltenberger subtype glycophorin variant found. Glycophorin variant St(a) was found from 35 out of 1027 samples (3.4%). In contrast to anti-'Mi(a)', which is the most frequently identified irregular antibody in Taiwan, the prevalence of anti-St(a) was only 0.13% as determined by serologic method. In addition, two new alleles for St(a) were found and reported.<h4>Conclusion/significance</h4>We confirm the long-standing assumption that GP.Mur is the only prevalent Miltenberger subtype in Taiwan. The current anti-'Mi(a)' screening method used in Taiwan, although neither sensitive nor specific, is still a suitable practice. Although St(a) antigen has a high prevalence in Taiwan, routine screening for anti-St(a) is not warranted based on current evidence.
format	article
author	Tai-Di Chen Ding-Ping Chen Wei-Ting Wang Chien-Feng Sun
author_facet	Tai-Di Chen Ding-Ping Chen Wei-Ting Wang Chien-Feng Sun
author_sort	Tai-Di Chen
title	MNSs blood group glycophorin variants in Taiwan: a genotype-serotype correlation study of 'Mi(a)' and St(a) with report of two new alleles for St(a).
title_short	MNSs blood group glycophorin variants in Taiwan: a genotype-serotype correlation study of 'Mi(a)' and St(a) with report of two new alleles for St(a).
title_full	MNSs blood group glycophorin variants in Taiwan: a genotype-serotype correlation study of 'Mi(a)' and St(a) with report of two new alleles for St(a).
title_fullStr	MNSs blood group glycophorin variants in Taiwan: a genotype-serotype correlation study of 'Mi(a)' and St(a) with report of two new alleles for St(a).
title_full_unstemmed	MNSs blood group glycophorin variants in Taiwan: a genotype-serotype correlation study of 'Mi(a)' and St(a) with report of two new alleles for St(a).
title_sort	mnss blood group glycophorin variants in taiwan: a genotype-serotype correlation study of 'mi(a)' and st(a) with report of two new alleles for st(a).
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/b751ec6b6887497ab5fd7f0d11a7099b
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MNSs blood group glycophorin variants in Taiwan: a genotype-serotype correlation study of 'Mi(a)' and St(a) with report of two new alleles for St(a).

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