Discovery of a new human polyomavirus associated with trichodysplasia spinulosa in an immunocompromized patient.

The Polyomaviridae constitute a family of small DNA viruses infecting a variety of hosts. In humans, polyomaviruses can cause infections of the central nervous system, urinary tract, skin, and possibly the respiratory tract. Here we report the identification of a new human polyomavirus in plucked fa...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Els van der Meijden, René W A Janssens, Chris Lauber, Jan Nico Bouwes Bavinck, Alexander E Gorbalenya, Mariet C W Feltkamp
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/b755d805c3734ffd8835e612aa56993e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b755d805c3734ffd8835e612aa56993e
record_format	dspace
spelling	oai:doaj.org-article:b755d805c3734ffd8835e612aa56993e2021-11-18T06:01:47ZDiscovery of a new human polyomavirus associated with trichodysplasia spinulosa in an immunocompromized patient.1553-73661553-737410.1371/journal.ppat.1001024https://doaj.org/article/b755d805c3734ffd8835e612aa56993e2010-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20686659/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374The Polyomaviridae constitute a family of small DNA viruses infecting a variety of hosts. In humans, polyomaviruses can cause infections of the central nervous system, urinary tract, skin, and possibly the respiratory tract. Here we report the identification of a new human polyomavirus in plucked facial spines of a heart transplant patient with trichodysplasia spinulosa, a rare skin disease exclusively seen in immunocompromized patients. The trichodysplasia spinulosa-associated polyomavirus (TSV) genome was amplified through rolling-circle amplification and consists of a 5232-nucleotide circular DNA organized similarly to known polyomaviruses. Two putative "early" (small and large T antigen) and three putative "late" (VP1, VP2, VP3) genes were identified. The TSV large T antigen contains several domains (e.g. J-domain) and motifs (e.g. HPDKGG, pRb family-binding, zinc finger) described for other polyomaviruses and potentially involved in cellular transformation. Phylogenetic analysis revealed a close relationship of TSV with the Bornean orangutan polyomavirus and, more distantly, the Merkel cell polyomavirus that is found integrated in Merkel cell carcinomas of the skin. The presence of TSV in the affected patient's skin was confirmed by newly designed quantitative TSV-specific PCR, indicative of a viral load of 10(5) copies per cell. After topical cidofovir treatment, the lesions largely resolved coinciding with a reduction in TSV load. PCR screening demonstrated a 4% prevalence of TSV in an unrelated group of immunosuppressed transplant recipients without apparent disease. In conclusion, a new human polyomavirus was discovered and identified as the possible cause of trichodysplasia spinulosa in immunocompromized patients. The presence of TSV also in clinically unaffected individuals suggests frequent virus transmission causing subclinical, probably latent infections. Further studies have to reveal the impact of TSV infection in relation to other populations and diseases.Els van der MeijdenRené W A JanssensChris LauberJan Nico Bouwes BavinckAlexander E GorbalenyaMariet C W FeltkampPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 6, Iss 7, p e1001024 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Els van der Meijden René W A Janssens Chris Lauber Jan Nico Bouwes Bavinck Alexander E Gorbalenya Mariet C W Feltkamp Discovery of a new human polyomavirus associated with trichodysplasia spinulosa in an immunocompromized patient.
description	The Polyomaviridae constitute a family of small DNA viruses infecting a variety of hosts. In humans, polyomaviruses can cause infections of the central nervous system, urinary tract, skin, and possibly the respiratory tract. Here we report the identification of a new human polyomavirus in plucked facial spines of a heart transplant patient with trichodysplasia spinulosa, a rare skin disease exclusively seen in immunocompromized patients. The trichodysplasia spinulosa-associated polyomavirus (TSV) genome was amplified through rolling-circle amplification and consists of a 5232-nucleotide circular DNA organized similarly to known polyomaviruses. Two putative "early" (small and large T antigen) and three putative "late" (VP1, VP2, VP3) genes were identified. The TSV large T antigen contains several domains (e.g. J-domain) and motifs (e.g. HPDKGG, pRb family-binding, zinc finger) described for other polyomaviruses and potentially involved in cellular transformation. Phylogenetic analysis revealed a close relationship of TSV with the Bornean orangutan polyomavirus and, more distantly, the Merkel cell polyomavirus that is found integrated in Merkel cell carcinomas of the skin. The presence of TSV in the affected patient's skin was confirmed by newly designed quantitative TSV-specific PCR, indicative of a viral load of 10(5) copies per cell. After topical cidofovir treatment, the lesions largely resolved coinciding with a reduction in TSV load. PCR screening demonstrated a 4% prevalence of TSV in an unrelated group of immunosuppressed transplant recipients without apparent disease. In conclusion, a new human polyomavirus was discovered and identified as the possible cause of trichodysplasia spinulosa in immunocompromized patients. The presence of TSV also in clinically unaffected individuals suggests frequent virus transmission causing subclinical, probably latent infections. Further studies have to reveal the impact of TSV infection in relation to other populations and diseases.
format	article
author	Els van der Meijden René W A Janssens Chris Lauber Jan Nico Bouwes Bavinck Alexander E Gorbalenya Mariet C W Feltkamp
author_facet	Els van der Meijden René W A Janssens Chris Lauber Jan Nico Bouwes Bavinck Alexander E Gorbalenya Mariet C W Feltkamp
author_sort	Els van der Meijden
title	Discovery of a new human polyomavirus associated with trichodysplasia spinulosa in an immunocompromized patient.
title_short	Discovery of a new human polyomavirus associated with trichodysplasia spinulosa in an immunocompromized patient.
title_full	Discovery of a new human polyomavirus associated with trichodysplasia spinulosa in an immunocompromized patient.
title_fullStr	Discovery of a new human polyomavirus associated with trichodysplasia spinulosa in an immunocompromized patient.
title_full_unstemmed	Discovery of a new human polyomavirus associated with trichodysplasia spinulosa in an immunocompromized patient.
title_sort	discovery of a new human polyomavirus associated with trichodysplasia spinulosa in an immunocompromized patient.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/b755d805c3734ffd8835e612aa56993e
work_keys_str_mv	AT elsvandermeijden discoveryofanewhumanpolyomavirusassociatedwithtrichodysplasiaspinulosainanimmunocompromizedpatient AT renewajanssens discoveryofanewhumanpolyomavirusassociatedwithtrichodysplasiaspinulosainanimmunocompromizedpatient AT chrislauber discoveryofanewhumanpolyomavirusassociatedwithtrichodysplasiaspinulosainanimmunocompromizedpatient AT jannicobouwesbavinck discoveryofanewhumanpolyomavirusassociatedwithtrichodysplasiaspinulosainanimmunocompromizedpatient AT alexanderegorbalenya discoveryofanewhumanpolyomavirusassociatedwithtrichodysplasiaspinulosainanimmunocompromizedpatient AT marietcwfeltkamp discoveryofanewhumanpolyomavirusassociatedwithtrichodysplasiaspinulosainanimmunocompromizedpatient
_version_	1718424688967811072

Discovery of a new human polyomavirus associated with trichodysplasia spinulosa in an immunocompromized patient.

Ejemplares similares