Electromagnetic stimulation increases mitochondrial function in osteogenic cells and promotes bone fracture repair

Electromagnetic stimulation increases mitochondrial function in osteogenic cells and promotes bone fracture repair

Abstract Bone fracture is a growing public health burden and there is a clinical need for non-invasive therapies to aid in the fracture healing process. Previous studies have demonstrated the utility of electromagnetic (EM) fields in promoting bone repair; however, its underlying mechanism of action...

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Autores principales: Alex M. Hollenberg, Aric Huber, Charles O. Smith, Roman A. Eliseev
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/b76915e396084415b4d89df82a9710b6
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spelling oai:doaj.org-article:b76915e396084415b4d89df82a9710b62021-12-02T19:17:05ZElectromagnetic stimulation increases mitochondrial function in osteogenic cells and promotes bone fracture repair10.1038/s41598-021-98625-12045-2322https://doaj.org/article/b76915e396084415b4d89df82a9710b62021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98625-1https://doaj.org/toc/2045-2322Abstract Bone fracture is a growing public health burden and there is a clinical need for non-invasive therapies to aid in the fracture healing process. Previous studies have demonstrated the utility of electromagnetic (EM) fields in promoting bone repair; however, its underlying mechanism of action is unclear. Interestingly, there is a growing body of literature describing positive effects of an EM field on mitochondria. In our own work, we have previously demonstrated that differentiation of osteoprogenitors into osteoblasts involves activation of mitochondrial oxidative phosphorylation (OxPhos). Therefore, it was reasonable to propose that EM field therapy exerts bone anabolic effects via stimulation of mitochondrial OxPhos. In this study, we show that application of a low intensity constant EM field source on osteogenic cells in vitro resulted in increased mitochondrial membrane potential and respiratory complex I activity and induced osteogenic differentiation. In the presence of mitochondrial inhibitor antimycin A, the osteoinductive effect was reversed, confirming that this effect was mediated via increased OxPhos activity. Using a mouse tibial bone fracture model in vivo, we show that application of a low intensity constant EM field source enhanced fracture repair via improved biomechanical properties and increased callus bone mineralization. Overall, this study provides supporting evidence that EM field therapy promotes bone fracture repair through mitochondrial OxPhos activation.Alex M. HollenbergAric HuberCharles O. SmithRoman A. EliseevNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Alex M. Hollenberg
Aric Huber
Charles O. Smith
Roman A. Eliseev
Electromagnetic stimulation increases mitochondrial function in osteogenic cells and promotes bone fracture repair
description Abstract Bone fracture is a growing public health burden and there is a clinical need for non-invasive therapies to aid in the fracture healing process. Previous studies have demonstrated the utility of electromagnetic (EM) fields in promoting bone repair; however, its underlying mechanism of action is unclear. Interestingly, there is a growing body of literature describing positive effects of an EM field on mitochondria. In our own work, we have previously demonstrated that differentiation of osteoprogenitors into osteoblasts involves activation of mitochondrial oxidative phosphorylation (OxPhos). Therefore, it was reasonable to propose that EM field therapy exerts bone anabolic effects via stimulation of mitochondrial OxPhos. In this study, we show that application of a low intensity constant EM field source on osteogenic cells in vitro resulted in increased mitochondrial membrane potential and respiratory complex I activity and induced osteogenic differentiation. In the presence of mitochondrial inhibitor antimycin A, the osteoinductive effect was reversed, confirming that this effect was mediated via increased OxPhos activity. Using a mouse tibial bone fracture model in vivo, we show that application of a low intensity constant EM field source enhanced fracture repair via improved biomechanical properties and increased callus bone mineralization. Overall, this study provides supporting evidence that EM field therapy promotes bone fracture repair through mitochondrial OxPhos activation.
format article
author Alex M. Hollenberg
Aric Huber
Charles O. Smith
Roman A. Eliseev
author_facet Alex M. Hollenberg
Aric Huber
Charles O. Smith
Roman A. Eliseev
author_sort Alex M. Hollenberg
title Electromagnetic stimulation increases mitochondrial function in osteogenic cells and promotes bone fracture repair
title_short Electromagnetic stimulation increases mitochondrial function in osteogenic cells and promotes bone fracture repair
title_full Electromagnetic stimulation increases mitochondrial function in osteogenic cells and promotes bone fracture repair
title_fullStr Electromagnetic stimulation increases mitochondrial function in osteogenic cells and promotes bone fracture repair
title_full_unstemmed Electromagnetic stimulation increases mitochondrial function in osteogenic cells and promotes bone fracture repair
title_sort electromagnetic stimulation increases mitochondrial function in osteogenic cells and promotes bone fracture repair
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/b76915e396084415b4d89df82a9710b6
work_keys_str_mv AT alexmhollenberg electromagneticstimulationincreasesmitochondrialfunctioninosteogeniccellsandpromotesbonefracturerepair
AT arichuber electromagneticstimulationincreasesmitochondrialfunctioninosteogeniccellsandpromotesbonefracturerepair
AT charlesosmith electromagneticstimulationincreasesmitochondrialfunctioninosteogeniccellsandpromotesbonefracturerepair
AT romanaeliseev electromagneticstimulationincreasesmitochondrialfunctioninosteogeniccellsandpromotesbonefracturerepair
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