The Isolation of Culturable Bacteria in <i>Ixodes ricinus</i> Ticks of a Belgian Peri-Urban Forest Uncovers Opportunistic Bacteria Potentially Important for Public Health

Most bacteria found in ticks are not pathogenic to humans but coexist as endosymbionts and may have effects on tick fitness and pathogen transmission. In this study, we cultured and isolated 78 bacteria from 954 <i>Ixodes ricinus</i> ticks collected in 7 sites of a Belgian peri-urban for...

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Autores principales:	Raphaël Rousseau, Sophie O. Vanwambeke, Cécile Boland, Marcella Mori
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Ixodes ricinus</i> microbiota bacterial flora <i>Cedecea davisae</i> species diversity Medicine R
Acceso en línea:	https://doaj.org/article/b77167508e7b424b90972dd991a89311
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spelling	oai:doaj.org-article:b77167508e7b424b90972dd991a893112021-11-25T17:51:24ZThe Isolation of Culturable Bacteria in <i>Ixodes ricinus</i> Ticks of a Belgian Peri-Urban Forest Uncovers Opportunistic Bacteria Potentially Important for Public Health10.3390/ijerph1822121341660-46011661-7827https://doaj.org/article/b77167508e7b424b90972dd991a893112021-11-01T00:00:00Zhttps://www.mdpi.com/1660-4601/18/22/12134https://doaj.org/toc/1661-7827https://doaj.org/toc/1660-4601Most bacteria found in ticks are not pathogenic to humans but coexist as endosymbionts and may have effects on tick fitness and pathogen transmission. In this study, we cultured and isolated 78 bacteria from 954 <i>Ixodes ricinus</i> ticks collected in 7 sites of a Belgian peri-urban forest. Most isolated species were non-pathogenic environmental microorganisms, and were from the Firmicutes (69.23%), Actinobacteria (17.95%) and Proteobacteria (3.84%) phyla. One bacterium isolate was particularly noteworthy, <i>Cedecea davisae</i>, a rare opportunistic bacterium, naturally resistant to various antibiotics. It has never been isolated from ticks before and this isolated strain was resistant to ampicillin, cefoxitin and colistin. Although cultivable bacteria do not represent the complete tick microbiota, the sites presented variable bacterial compositions and diversities. This study is a first attempt to describe the culturable microbiota of ticks collected in Belgium. Further collections and analyses of ticks of different species, from various areas and using other bacterial identification methods would strengthen these results. However, they highlight the importance of ticks as potential sentinel for opportunistic bacteria of public health importance.Raphaël RousseauSophie O. VanwambekeCécile BolandMarcella MoriMDPI AGarticle<i>Ixodes ricinus</i>microbiotabacterial flora<i>Cedecea davisae</i>species diversityMedicineRENInternational Journal of Environmental Research and Public Health, Vol 18, Iss 12134, p 12134 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Ixodes ricinus</i> microbiota bacterial flora <i>Cedecea davisae</i> species diversity Medicine R
spellingShingle	<i>Ixodes ricinus</i> microbiota bacterial flora <i>Cedecea davisae</i> species diversity Medicine R Raphaël Rousseau Sophie O. Vanwambeke Cécile Boland Marcella Mori The Isolation of Culturable Bacteria in <i>Ixodes ricinus</i> Ticks of a Belgian Peri-Urban Forest Uncovers Opportunistic Bacteria Potentially Important for Public Health
description	Most bacteria found in ticks are not pathogenic to humans but coexist as endosymbionts and may have effects on tick fitness and pathogen transmission. In this study, we cultured and isolated 78 bacteria from 954 <i>Ixodes ricinus</i> ticks collected in 7 sites of a Belgian peri-urban forest. Most isolated species were non-pathogenic environmental microorganisms, and were from the Firmicutes (69.23%), Actinobacteria (17.95%) and Proteobacteria (3.84%) phyla. One bacterium isolate was particularly noteworthy, <i>Cedecea davisae</i>, a rare opportunistic bacterium, naturally resistant to various antibiotics. It has never been isolated from ticks before and this isolated strain was resistant to ampicillin, cefoxitin and colistin. Although cultivable bacteria do not represent the complete tick microbiota, the sites presented variable bacterial compositions and diversities. This study is a first attempt to describe the culturable microbiota of ticks collected in Belgium. Further collections and analyses of ticks of different species, from various areas and using other bacterial identification methods would strengthen these results. However, they highlight the importance of ticks as potential sentinel for opportunistic bacteria of public health importance.
format	article
author	Raphaël Rousseau Sophie O. Vanwambeke Cécile Boland Marcella Mori
author_facet	Raphaël Rousseau Sophie O. Vanwambeke Cécile Boland Marcella Mori
author_sort	Raphaël Rousseau
title	The Isolation of Culturable Bacteria in <i>Ixodes ricinus</i> Ticks of a Belgian Peri-Urban Forest Uncovers Opportunistic Bacteria Potentially Important for Public Health
title_short	The Isolation of Culturable Bacteria in <i>Ixodes ricinus</i> Ticks of a Belgian Peri-Urban Forest Uncovers Opportunistic Bacteria Potentially Important for Public Health
title_full	The Isolation of Culturable Bacteria in <i>Ixodes ricinus</i> Ticks of a Belgian Peri-Urban Forest Uncovers Opportunistic Bacteria Potentially Important for Public Health
title_fullStr	The Isolation of Culturable Bacteria in <i>Ixodes ricinus</i> Ticks of a Belgian Peri-Urban Forest Uncovers Opportunistic Bacteria Potentially Important for Public Health
title_full_unstemmed	The Isolation of Culturable Bacteria in <i>Ixodes ricinus</i> Ticks of a Belgian Peri-Urban Forest Uncovers Opportunistic Bacteria Potentially Important for Public Health
title_sort	isolation of culturable bacteria in <i>ixodes ricinus</i> ticks of a belgian peri-urban forest uncovers opportunistic bacteria potentially important for public health
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b77167508e7b424b90972dd991a89311
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The Isolation of Culturable Bacteria in <i>Ixodes ricinus</i> Ticks of a Belgian Peri-Urban Forest Uncovers Opportunistic Bacteria Potentially Important for Public Health

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