Electrospun vancomycin-loaded coating on titanium implants for the prevention of implant-associated infections

Li Zhang,* Junwei Yan,* Zhaowei Yin, Cheng Tang, Yang Guo, Dong Li, Bo Wei, Yan Xu, Qiangrong Gu, Liming Wang Orthopedic Laboratory of Nanjing First Hospital, Nanjing Medical University, Nanjing, People’s Republic of China *Both of these authors contributed equally to this work Abstrac...

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Autores principales:	Zhang L, Yan JW, Yin ZW, Tang C, Guo Y, Li D, Wei B, Xu Y, Gu QR, Wang LM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/b775610892764499818be85a9c7739b9
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spelling	oai:doaj.org-article:b775610892764499818be85a9c7739b92021-12-02T02:10:31ZElectrospun vancomycin-loaded coating on titanium implants for the prevention of implant-associated infections1178-2013https://doaj.org/article/b775610892764499818be85a9c7739b92014-06-01T00:00:00Zhttp://www.dovepress.com/electrospun-vancomycin-loaded-coating-on-titanium-implants-for-the-pre-a17329https://doaj.org/toc/1178-2013 Li Zhang,* Junwei Yan,* Zhaowei Yin, Cheng Tang, Yang Guo, Dong Li, Bo Wei, Yan Xu, Qiangrong Gu, Liming Wang Orthopedic Laboratory of Nanjing First Hospital, Nanjing Medical University, Nanjing, People’s Republic of China *Both of these authors contributed equally to this work Abstract: The objectives of this work were to develop an antibiotic coating on the surface of a titanium plate to determine its antibacterial properties in vitro and in vivo. To prepare vancomycin-coated titanium implants, we adopted the electrospinning nanotechnique. The surface structure of the coating implants was observed using a scanning electron microscope. An elution method and a high-pressure liquid chromatography assay were used to characterize the release behavior of vancomycin from the coating. The antibacterial efficacy and the cytotoxicity of the coated titanium implants on osteoblasts were investigated in vitro. In addition, X-ray, white blood cell count, C-reactive protein, erythrocyte sedimentation rate, and pathological examination were performed to validate its antimicrobial efficacy in vivo. The antibiotic coating released 82.7% (approximately 528.2 µg) of total vancomycin loading in the coating in vitro. The release behavior of vancomycin from nanofiber coatings exhibited a biphasic release pattern with an initial burst on day 1, followed by a slow and controlled release over 28 days. There was no cytotoxicity observed in vitro for the vancomycin-loaded coating. The vancomycin-coated titanium implants were active in treating implant-associated infection in vivo. Thus, vancomycin-coated titanium implants may be a promising approach to prevent and treat implant-associated infections. Keywords: electrospinning, vancomycin, implant, infection, coating, nanofibersZhang LYan JWYin ZWTang CGuo YLi DWei BXu YGu QRWang LMDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 3027-3036 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Zhang L Yan JW Yin ZW Tang C Guo Y Li D Wei B Xu Y Gu QR Wang LM Electrospun vancomycin-loaded coating on titanium implants for the prevention of implant-associated infections
description	Li Zhang,* Junwei Yan,* Zhaowei Yin, Cheng Tang, Yang Guo, Dong Li, Bo Wei, Yan Xu, Qiangrong Gu, Liming Wang Orthopedic Laboratory of Nanjing First Hospital, Nanjing Medical University, Nanjing, People’s Republic of China *Both of these authors contributed equally to this work Abstract: The objectives of this work were to develop an antibiotic coating on the surface of a titanium plate to determine its antibacterial properties in vitro and in vivo. To prepare vancomycin-coated titanium implants, we adopted the electrospinning nanotechnique. The surface structure of the coating implants was observed using a scanning electron microscope. An elution method and a high-pressure liquid chromatography assay were used to characterize the release behavior of vancomycin from the coating. The antibacterial efficacy and the cytotoxicity of the coated titanium implants on osteoblasts were investigated in vitro. In addition, X-ray, white blood cell count, C-reactive protein, erythrocyte sedimentation rate, and pathological examination were performed to validate its antimicrobial efficacy in vivo. The antibiotic coating released 82.7% (approximately 528.2 µg) of total vancomycin loading in the coating in vitro. The release behavior of vancomycin from nanofiber coatings exhibited a biphasic release pattern with an initial burst on day 1, followed by a slow and controlled release over 28 days. There was no cytotoxicity observed in vitro for the vancomycin-loaded coating. The vancomycin-coated titanium implants were active in treating implant-associated infection in vivo. Thus, vancomycin-coated titanium implants may be a promising approach to prevent and treat implant-associated infections. Keywords: electrospinning, vancomycin, implant, infection, coating, nanofibers
format	article
author	Zhang L Yan JW Yin ZW Tang C Guo Y Li D Wei B Xu Y Gu QR Wang LM
author_facet	Zhang L Yan JW Yin ZW Tang C Guo Y Li D Wei B Xu Y Gu QR Wang LM
author_sort	Zhang L
title	Electrospun vancomycin-loaded coating on titanium implants for the prevention of implant-associated infections
title_short	Electrospun vancomycin-loaded coating on titanium implants for the prevention of implant-associated infections
title_full	Electrospun vancomycin-loaded coating on titanium implants for the prevention of implant-associated infections
title_fullStr	Electrospun vancomycin-loaded coating on titanium implants for the prevention of implant-associated infections
title_full_unstemmed	Electrospun vancomycin-loaded coating on titanium implants for the prevention of implant-associated infections
title_sort	electrospun vancomycin-loaded coating on titanium implants for the prevention of implant-associated infections
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/b775610892764499818be85a9c7739b9
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Electrospun vancomycin-loaded coating on titanium implants for the prevention of implant-associated infections

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