A novel microfluidic 3D platform for culturing pancreatic ductal adenocarcinoma cells: comparison with in vitro cultures and in vivo xenografts

Abstract The integration of microfluidics and cell biology has reached a significant milestone with the development of “organ-on-chips”, smart technological platforms that, once applied to the study of human diseases, such as cancer, might ultimately contribute to design personalised treatments and...

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Autores principales:	Meike Beer, Nirmala Kuppalu, Matteo Stefanini, Holger Becker, Ingo Schulz, Sagar Manoli, Julia Schuette, Christian Schmees, Armando Casazza, Martin Stelzle, Annarosa Arcangeli
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b77582e7d6ef46bc9a4d1c073a90d0e6
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spelling	oai:doaj.org-article:b77582e7d6ef46bc9a4d1c073a90d0e62021-12-02T12:32:12ZA novel microfluidic 3D platform for culturing pancreatic ductal adenocarcinoma cells: comparison with in vitro cultures and in vivo xenografts10.1038/s41598-017-01256-82045-2322https://doaj.org/article/b77582e7d6ef46bc9a4d1c073a90d0e62017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01256-8https://doaj.org/toc/2045-2322Abstract The integration of microfluidics and cell biology has reached a significant milestone with the development of “organ-on-chips”, smart technological platforms that, once applied to the study of human diseases, such as cancer, might ultimately contribute to design personalised treatments and hence improve health outcomes. This paper reports that the combination of microfluidics and dielectrophoresis (DEP) allows to culture different pancreatic ductal adenocarcinoma (PDAC) human cell lines into a cyclic olefin polymer (COP) chamber (HepaChip®), enriched by the extracellular matrix (ECM) protein collagen. We show that PDAC cells cultured into the HepaChip® (1) are vital and grow, provided they properly attach to collagen; (2) show morphological appearance and growth characteristics closer to those of cells grown as spheroids than as classical 2 dimensional (2D) in vitro cultures. Finally, preliminary experiments show that PDAC cells respond to high doses of Cisplatin perfused through the chip. Overall, the present microfluidic platform could be exploited in the future for a personalised approach to PDAC.Meike BeerNirmala KuppaluMatteo StefaniniHolger BeckerIngo SchulzSagar ManoliJulia SchuetteChristian SchmeesArmando CasazzaMartin StelzleAnnarosa ArcangeliNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Meike Beer Nirmala Kuppalu Matteo Stefanini Holger Becker Ingo Schulz Sagar Manoli Julia Schuette Christian Schmees Armando Casazza Martin Stelzle Annarosa Arcangeli A novel microfluidic 3D platform for culturing pancreatic ductal adenocarcinoma cells: comparison with in vitro cultures and in vivo xenografts
description	Abstract The integration of microfluidics and cell biology has reached a significant milestone with the development of “organ-on-chips”, smart technological platforms that, once applied to the study of human diseases, such as cancer, might ultimately contribute to design personalised treatments and hence improve health outcomes. This paper reports that the combination of microfluidics and dielectrophoresis (DEP) allows to culture different pancreatic ductal adenocarcinoma (PDAC) human cell lines into a cyclic olefin polymer (COP) chamber (HepaChip®), enriched by the extracellular matrix (ECM) protein collagen. We show that PDAC cells cultured into the HepaChip® (1) are vital and grow, provided they properly attach to collagen; (2) show morphological appearance and growth characteristics closer to those of cells grown as spheroids than as classical 2 dimensional (2D) in vitro cultures. Finally, preliminary experiments show that PDAC cells respond to high doses of Cisplatin perfused through the chip. Overall, the present microfluidic platform could be exploited in the future for a personalised approach to PDAC.
format	article
author	Meike Beer Nirmala Kuppalu Matteo Stefanini Holger Becker Ingo Schulz Sagar Manoli Julia Schuette Christian Schmees Armando Casazza Martin Stelzle Annarosa Arcangeli
author_facet	Meike Beer Nirmala Kuppalu Matteo Stefanini Holger Becker Ingo Schulz Sagar Manoli Julia Schuette Christian Schmees Armando Casazza Martin Stelzle Annarosa Arcangeli
author_sort	Meike Beer
title	A novel microfluidic 3D platform for culturing pancreatic ductal adenocarcinoma cells: comparison with in vitro cultures and in vivo xenografts
title_short	A novel microfluidic 3D platform for culturing pancreatic ductal adenocarcinoma cells: comparison with in vitro cultures and in vivo xenografts
title_full	A novel microfluidic 3D platform for culturing pancreatic ductal adenocarcinoma cells: comparison with in vitro cultures and in vivo xenografts
title_fullStr	A novel microfluidic 3D platform for culturing pancreatic ductal adenocarcinoma cells: comparison with in vitro cultures and in vivo xenografts
title_full_unstemmed	A novel microfluidic 3D platform for culturing pancreatic ductal adenocarcinoma cells: comparison with in vitro cultures and in vivo xenografts
title_sort	novel microfluidic 3d platform for culturing pancreatic ductal adenocarcinoma cells: comparison with in vitro cultures and in vivo xenografts
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/b77582e7d6ef46bc9a4d1c073a90d0e6
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A novel microfluidic 3D platform for culturing pancreatic ductal adenocarcinoma cells: comparison with in vitro cultures and in vivo xenografts

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