Genetic diversity of Ongole Grade, Aceh, and Sumbawa cattle based on polymorphism on ND-5 fragment mitochondrial DNA using PCR-RFLP technique

Abstract. Sutarno, Zahrah S, Astirin OP, Herawati E, Setyawan AD. 2019. Genetic diversity of Ongole Grade, Aceh, and Sumbawa cattle based on polymorphism on ND-5 fragment mitochondrial DNA using PCR-RFLP technique. Biodiversitas 20: 783-788. Genetic diversity is the basis of livestock breeding becau...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	SUTARNO SUTARNO, SYARIFA ZAHRAH, OKID PARAMA ASTIRIN, ELISA HERAWATI, AHMAD DWI SETYAWAN
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/b7765c8f92114080b2fd798394c8eb38
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b7765c8f92114080b2fd798394c8eb38
record_format	dspace
spelling	oai:doaj.org-article:b7765c8f92114080b2fd798394c8eb382021-11-16T14:12:15ZGenetic diversity of Ongole Grade, Aceh, and Sumbawa cattle based on polymorphism on ND-5 fragment mitochondrial DNA using PCR-RFLP technique1412-033X2085-472210.13057/biodiv/d200324https://doaj.org/article/b7765c8f92114080b2fd798394c8eb382019-03-01T00:00:00Zhttps://smujo.id/biodiv/article/view/3632https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Sutarno, Zahrah S, Astirin OP, Herawati E, Setyawan AD. 2019. Genetic diversity of Ongole Grade, Aceh, and Sumbawa cattle based on polymorphism on ND-5 fragment mitochondrial DNA using PCR-RFLP technique. Biodiversitas 20: 783-788. Genetic diversity is the basis of livestock breeding because it can be used as an initial improvement in livestock quality through artificial selection. This study aims to determine polymorphism in ND-5 fragment of mitochondrial DNA in Ongole Grade, Aceh, and Sumbawa cattle and their genetic diversity. The total DNA from the blood of the local cattle was extracted using the Wizard genomic DNA purification system from Promega and amplified using the PCR technique. The PCR product was then digested with HindIII enzyme using the RFLP technique to detect polymorphism. The genetic diversity of the Ongole Grade, Aceh, and Sumbawa cattle was analyzed using the formula from Nei and its genetic relationship was evaluated with the 2.02i NTSYSpc version program. Our findings showed there were polymorphisms in the ND-5 fragment of mitochondrial DNA. Digestion with HindIII restriction enzyme produces two types of haplotypes. Haplotype B is a 453 bp-sized DNA fragment that is not truncated by the HindIII enzyme, and haplotype A is a DNA fragment cut by HindIII enzyme into two with fragments of 336 bp and 117 bp. Polymorphism was found in Ongole Grade cattle, but not in Sumbawa and Aceh cattle. Haplotypes diversity in ND-5 fragments of mitochondrial DNA of Ongole Grade was 0.6250 while Sumbawa and Aceh cattle displayed no diversity of haplotypes. The genetic relationship shows that Sumbawa cattle belonged to the same cluster with Ongole Grade but separated from Aceh cattle.SUTARNO SUTARNOSYARIFA ZAHRAHOKID PARAMA ASTIRINELISA HERAWATIAHMAD DWI SETYAWANMBI & UNS SoloarticleBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 3, Pp 783-788 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 SUTARNO SUTARNO SYARIFA ZAHRAH OKID PARAMA ASTIRIN ELISA HERAWATI AHMAD DWI SETYAWAN Genetic diversity of Ongole Grade, Aceh, and Sumbawa cattle based on polymorphism on ND-5 fragment mitochondrial DNA using PCR-RFLP technique
description	Abstract. Sutarno, Zahrah S, Astirin OP, Herawati E, Setyawan AD. 2019. Genetic diversity of Ongole Grade, Aceh, and Sumbawa cattle based on polymorphism on ND-5 fragment mitochondrial DNA using PCR-RFLP technique. Biodiversitas 20: 783-788. Genetic diversity is the basis of livestock breeding because it can be used as an initial improvement in livestock quality through artificial selection. This study aims to determine polymorphism in ND-5 fragment of mitochondrial DNA in Ongole Grade, Aceh, and Sumbawa cattle and their genetic diversity. The total DNA from the blood of the local cattle was extracted using the Wizard genomic DNA purification system from Promega and amplified using the PCR technique. The PCR product was then digested with HindIII enzyme using the RFLP technique to detect polymorphism. The genetic diversity of the Ongole Grade, Aceh, and Sumbawa cattle was analyzed using the formula from Nei and its genetic relationship was evaluated with the 2.02i NTSYSpc version program. Our findings showed there were polymorphisms in the ND-5 fragment of mitochondrial DNA. Digestion with HindIII restriction enzyme produces two types of haplotypes. Haplotype B is a 453 bp-sized DNA fragment that is not truncated by the HindIII enzyme, and haplotype A is a DNA fragment cut by HindIII enzyme into two with fragments of 336 bp and 117 bp. Polymorphism was found in Ongole Grade cattle, but not in Sumbawa and Aceh cattle. Haplotypes diversity in ND-5 fragments of mitochondrial DNA of Ongole Grade was 0.6250 while Sumbawa and Aceh cattle displayed no diversity of haplotypes. The genetic relationship shows that Sumbawa cattle belonged to the same cluster with Ongole Grade but separated from Aceh cattle.
format	article
author	SUTARNO SUTARNO SYARIFA ZAHRAH OKID PARAMA ASTIRIN ELISA HERAWATI AHMAD DWI SETYAWAN
author_facet	SUTARNO SUTARNO SYARIFA ZAHRAH OKID PARAMA ASTIRIN ELISA HERAWATI AHMAD DWI SETYAWAN
author_sort	SUTARNO SUTARNO
title	Genetic diversity of Ongole Grade, Aceh, and Sumbawa cattle based on polymorphism on ND-5 fragment mitochondrial DNA using PCR-RFLP technique
title_short	Genetic diversity of Ongole Grade, Aceh, and Sumbawa cattle based on polymorphism on ND-5 fragment mitochondrial DNA using PCR-RFLP technique
title_full	Genetic diversity of Ongole Grade, Aceh, and Sumbawa cattle based on polymorphism on ND-5 fragment mitochondrial DNA using PCR-RFLP technique
title_fullStr	Genetic diversity of Ongole Grade, Aceh, and Sumbawa cattle based on polymorphism on ND-5 fragment mitochondrial DNA using PCR-RFLP technique
title_full_unstemmed	Genetic diversity of Ongole Grade, Aceh, and Sumbawa cattle based on polymorphism on ND-5 fragment mitochondrial DNA using PCR-RFLP technique
title_sort	genetic diversity of ongole grade, aceh, and sumbawa cattle based on polymorphism on nd-5 fragment mitochondrial dna using pcr-rflp technique
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/b7765c8f92114080b2fd798394c8eb38
work_keys_str_mv	AT sutarnosutarno geneticdiversityofongolegradeacehandsumbawacattlebasedonpolymorphismonnd5fragmentmitochondrialdnausingpcrrflptechnique AT syarifazahrah geneticdiversityofongolegradeacehandsumbawacattlebasedonpolymorphismonnd5fragmentmitochondrialdnausingpcrrflptechnique AT okidparamaastirin geneticdiversityofongolegradeacehandsumbawacattlebasedonpolymorphismonnd5fragmentmitochondrialdnausingpcrrflptechnique AT elisaherawati geneticdiversityofongolegradeacehandsumbawacattlebasedonpolymorphismonnd5fragmentmitochondrialdnausingpcrrflptechnique AT ahmaddwisetyawan geneticdiversityofongolegradeacehandsumbawacattlebasedonpolymorphismonnd5fragmentmitochondrialdnausingpcrrflptechnique
_version_	1718426381528858624

Genetic diversity of Ongole Grade, Aceh, and Sumbawa cattle based on polymorphism on ND-5 fragment mitochondrial DNA using PCR-RFLP technique

Ejemplares similares