Wnt5A modulates integrin expression in a receptor-dependent manner in ovarian cancer cells

Abstract Wnt5A signals through various receptors that confer versatile biological functions. Here, we used Wnt5A overexpressing human ovarian SKOV-3 and OVCAR-3 stable clones for assessing integrin expression, cell proliferation, migration, invasion, and the ability of multicellular aggregates (MCAs...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Vajihe Azimian-Zavareh, Zeinab Dehghani-Ghobadi, Marzieh Ebrahimi, Kian Mirzazadeh, Irina Nazarenko, Ghamartaj Hossein
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b7767adc982e415e8a35af79b5a07103
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b7767adc982e415e8a35af79b5a07103
record_format	dspace
spelling	oai:doaj.org-article:b7767adc982e415e8a35af79b5a071032021-12-02T17:05:46ZWnt5A modulates integrin expression in a receptor-dependent manner in ovarian cancer cells10.1038/s41598-021-85356-62045-2322https://doaj.org/article/b7767adc982e415e8a35af79b5a071032021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85356-6https://doaj.org/toc/2045-2322Abstract Wnt5A signals through various receptors that confer versatile biological functions. Here, we used Wnt5A overexpressing human ovarian SKOV-3 and OVCAR-3 stable clones for assessing integrin expression, cell proliferation, migration, invasion, and the ability of multicellular aggregates (MCAs) formation. We found here, that Wnt5A regulates differently the expression of its receptors in the stable Wnt5A overexpressing clones. The expression levels of Frizzled (FZD)-2 and -5, were increased in different clones. However ROR-1, -2 expression levels were differently regulated in clones. Wnt5A overexpressing clones showed increased cell proliferation, migration, and clonogenicity. Moreover, Wnt5A overexpressing SKOV-3 clone showed increased MCAs formation ability. Cell invasion had been increased in OVCAR-3-derived clones, while this was decreased in SKOV-3-derived clone. Importantly, αv integrin expression levels were increased in all assessed clones, accompanied by increased cell attachment to fibronectin and focal adhesion kinase activity. Moreover, the treatment of clones with Box5 as a Wnt5A/FZD5 antagonist abrogates ITGAV increase, cell proliferation, migration, and their attachment to fibronectin. Accordingly, we observed significantly higher expression levels of ITGAV and ITGB3 in human high-grade serous ovarian cancer specimens and ITGAV correlated positively with Wnt5A in metastatic serous type ovarian cancer. In summary, we hypothesize here, that Wnt5A/FZD-5 signaling modulate αv integrin expression levels that could be associated with ovarian cancer cell proliferation, migration, and fibronectin attachment.Vajihe Azimian-ZavarehZeinab Dehghani-GhobadiMarzieh EbrahimiKian MirzazadehIrina NazarenkoGhamartaj HosseinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vajihe Azimian-Zavareh Zeinab Dehghani-Ghobadi Marzieh Ebrahimi Kian Mirzazadeh Irina Nazarenko Ghamartaj Hossein Wnt5A modulates integrin expression in a receptor-dependent manner in ovarian cancer cells
description	Abstract Wnt5A signals through various receptors that confer versatile biological functions. Here, we used Wnt5A overexpressing human ovarian SKOV-3 and OVCAR-3 stable clones for assessing integrin expression, cell proliferation, migration, invasion, and the ability of multicellular aggregates (MCAs) formation. We found here, that Wnt5A regulates differently the expression of its receptors in the stable Wnt5A overexpressing clones. The expression levels of Frizzled (FZD)-2 and -5, were increased in different clones. However ROR-1, -2 expression levels were differently regulated in clones. Wnt5A overexpressing clones showed increased cell proliferation, migration, and clonogenicity. Moreover, Wnt5A overexpressing SKOV-3 clone showed increased MCAs formation ability. Cell invasion had been increased in OVCAR-3-derived clones, while this was decreased in SKOV-3-derived clone. Importantly, αv integrin expression levels were increased in all assessed clones, accompanied by increased cell attachment to fibronectin and focal adhesion kinase activity. Moreover, the treatment of clones with Box5 as a Wnt5A/FZD5 antagonist abrogates ITGAV increase, cell proliferation, migration, and their attachment to fibronectin. Accordingly, we observed significantly higher expression levels of ITGAV and ITGB3 in human high-grade serous ovarian cancer specimens and ITGAV correlated positively with Wnt5A in metastatic serous type ovarian cancer. In summary, we hypothesize here, that Wnt5A/FZD-5 signaling modulate αv integrin expression levels that could be associated with ovarian cancer cell proliferation, migration, and fibronectin attachment.
format	article
author	Vajihe Azimian-Zavareh Zeinab Dehghani-Ghobadi Marzieh Ebrahimi Kian Mirzazadeh Irina Nazarenko Ghamartaj Hossein
author_facet	Vajihe Azimian-Zavareh Zeinab Dehghani-Ghobadi Marzieh Ebrahimi Kian Mirzazadeh Irina Nazarenko Ghamartaj Hossein
author_sort	Vajihe Azimian-Zavareh
title	Wnt5A modulates integrin expression in a receptor-dependent manner in ovarian cancer cells
title_short	Wnt5A modulates integrin expression in a receptor-dependent manner in ovarian cancer cells
title_full	Wnt5A modulates integrin expression in a receptor-dependent manner in ovarian cancer cells
title_fullStr	Wnt5A modulates integrin expression in a receptor-dependent manner in ovarian cancer cells
title_full_unstemmed	Wnt5A modulates integrin expression in a receptor-dependent manner in ovarian cancer cells
title_sort	wnt5a modulates integrin expression in a receptor-dependent manner in ovarian cancer cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b7767adc982e415e8a35af79b5a07103
work_keys_str_mv	AT vajiheazimianzavareh wnt5amodulatesintegrinexpressioninareceptordependentmannerinovariancancercells AT zeinabdehghanighobadi wnt5amodulatesintegrinexpressioninareceptordependentmannerinovariancancercells AT marziehebrahimi wnt5amodulatesintegrinexpressioninareceptordependentmannerinovariancancercells AT kianmirzazadeh wnt5amodulatesintegrinexpressioninareceptordependentmannerinovariancancercells AT irinanazarenko wnt5amodulatesintegrinexpressioninareceptordependentmannerinovariancancercells AT ghamartajhossein wnt5amodulatesintegrinexpressioninareceptordependentmannerinovariancancercells
_version_	1718381811711606784

Wnt5A modulates integrin expression in a receptor-dependent manner in ovarian cancer cells

Ejemplares similares