Fermentative hydrogen production from Jerusalem artichoke by Clostridium tyrobutyricum expressing exo-inulinase gene

Abstract Clostridium tyrobutyricum ATCC25755 has been reported as being able to produce significant quantities of hydrogen. In this study, the exo-inulinase encoding gene cloned from Paenibacillus polymyxa SC-2 was into the expression plasmid pSY6 and expressed in the cells of C. tyrobutyricum. The...

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Autores principales:	Ling Jiang, Qian Wu, Qing Xu, Liying Zhu, He Huang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b77dcf2a95e24f0bbf7be865b116b97c
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spelling	oai:doaj.org-article:b77dcf2a95e24f0bbf7be865b116b97c2021-12-02T11:53:09ZFermentative hydrogen production from Jerusalem artichoke by Clostridium tyrobutyricum expressing exo-inulinase gene10.1038/s41598-017-07207-72045-2322https://doaj.org/article/b77dcf2a95e24f0bbf7be865b116b97c2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07207-7https://doaj.org/toc/2045-2322Abstract Clostridium tyrobutyricum ATCC25755 has been reported as being able to produce significant quantities of hydrogen. In this study, the exo-inulinase encoding gene cloned from Paenibacillus polymyxa SC-2 was into the expression plasmid pSY6 and expressed in the cells of C. tyrobutyricum. The engineered C. tyrobutyricum strain efficiently fermented the inulin-type carbohydrates from Jerusalem artichoke, without any pretreatment being necessary for the production of hydrogen. A comparatively high hydrogen yield (3.7 mol/mol inulin-type sugar) was achieved after 96 h in a batch process with simultaneous saccharification and fermentation (SSF), with an overall volumetric productivity rate of 620 ± 60 mL/h/L when the initial total sugar concentration of the inulin extract was increased to 100 g/L. Synthesis of inulinase in the batch SSF culture was closely associated with strain growth until the end of the exponential phase, reaching a maximum activity of 28.4 ± 0.26 U/mL. The overall results show that the highly productive and abundant biomass crop Jerusalem artichoke can be a good substrate for hydrogen production, and that the application of batch SSF for its conversion has the potential to become a cost-effective process in the near future.Ling JiangQian WuQing XuLiying ZhuHe HuangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ling Jiang Qian Wu Qing Xu Liying Zhu He Huang Fermentative hydrogen production from Jerusalem artichoke by Clostridium tyrobutyricum expressing exo-inulinase gene
description	Abstract Clostridium tyrobutyricum ATCC25755 has been reported as being able to produce significant quantities of hydrogen. In this study, the exo-inulinase encoding gene cloned from Paenibacillus polymyxa SC-2 was into the expression plasmid pSY6 and expressed in the cells of C. tyrobutyricum. The engineered C. tyrobutyricum strain efficiently fermented the inulin-type carbohydrates from Jerusalem artichoke, without any pretreatment being necessary for the production of hydrogen. A comparatively high hydrogen yield (3.7 mol/mol inulin-type sugar) was achieved after 96 h in a batch process with simultaneous saccharification and fermentation (SSF), with an overall volumetric productivity rate of 620 ± 60 mL/h/L when the initial total sugar concentration of the inulin extract was increased to 100 g/L. Synthesis of inulinase in the batch SSF culture was closely associated with strain growth until the end of the exponential phase, reaching a maximum activity of 28.4 ± 0.26 U/mL. The overall results show that the highly productive and abundant biomass crop Jerusalem artichoke can be a good substrate for hydrogen production, and that the application of batch SSF for its conversion has the potential to become a cost-effective process in the near future.
format	article
author	Ling Jiang Qian Wu Qing Xu Liying Zhu He Huang
author_facet	Ling Jiang Qian Wu Qing Xu Liying Zhu He Huang
author_sort	Ling Jiang
title	Fermentative hydrogen production from Jerusalem artichoke by Clostridium tyrobutyricum expressing exo-inulinase gene
title_short	Fermentative hydrogen production from Jerusalem artichoke by Clostridium tyrobutyricum expressing exo-inulinase gene
title_full	Fermentative hydrogen production from Jerusalem artichoke by Clostridium tyrobutyricum expressing exo-inulinase gene
title_fullStr	Fermentative hydrogen production from Jerusalem artichoke by Clostridium tyrobutyricum expressing exo-inulinase gene
title_full_unstemmed	Fermentative hydrogen production from Jerusalem artichoke by Clostridium tyrobutyricum expressing exo-inulinase gene
title_sort	fermentative hydrogen production from jerusalem artichoke by clostridium tyrobutyricum expressing exo-inulinase gene
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/b77dcf2a95e24f0bbf7be865b116b97c
work_keys_str_mv	AT lingjiang fermentativehydrogenproductionfromjerusalemartichokebyclostridiumtyrobutyricumexpressingexoinulinasegene AT qianwu fermentativehydrogenproductionfromjerusalemartichokebyclostridiumtyrobutyricumexpressingexoinulinasegene AT qingxu fermentativehydrogenproductionfromjerusalemartichokebyclostridiumtyrobutyricumexpressingexoinulinasegene AT liyingzhu fermentativehydrogenproductionfromjerusalemartichokebyclostridiumtyrobutyricumexpressingexoinulinasegene AT hehuang fermentativehydrogenproductionfromjerusalemartichokebyclostridiumtyrobutyricumexpressingexoinulinasegene
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Fermentative hydrogen production from Jerusalem artichoke by Clostridium tyrobutyricum expressing exo-inulinase gene

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