Noncytotoxic silver nanoparticles as a new antimicrobial strategy

Abstract Drug-resistance of bacteria is an ongoing problem in hospital treatment. The main mechanism of bacterial virulency in human infections is based on their adhesion ability and biofilm formation. Many approaches have been invented to overcome this problem, i.e. treatment with antibacterial bio...

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Autores principales:	Bartosz Skóra, Urszula Krajewska, Anna Nowak, Andrzej Dziedzic, Adriana Barylyak, Małgorzata Kus-Liśkiewicz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b77eb6939a15475ba4c1468ff3a240c0
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spelling	oai:doaj.org-article:b77eb6939a15475ba4c1468ff3a240c02021-12-02T16:32:12ZNoncytotoxic silver nanoparticles as a new antimicrobial strategy10.1038/s41598-021-92812-w2045-2322https://doaj.org/article/b77eb6939a15475ba4c1468ff3a240c02021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92812-whttps://doaj.org/toc/2045-2322Abstract Drug-resistance of bacteria is an ongoing problem in hospital treatment. The main mechanism of bacterial virulency in human infections is based on their adhesion ability and biofilm formation. Many approaches have been invented to overcome this problem, i.e. treatment with antibacterial biomolecules, which have some limitations e.g. enzymatic degradation and short shelf stability. Silver nanoparticles (AgNPs) may be alternative to these strategies due to their unique and high antibacterial properties. Herein, we report on yeast Saccharomyces cerevisiae extracellular-based synthesis of AgNPs. Transmission electron microscopy (TEM) revealed the morphology and structure of the metallic nanoparticles, which showed a uniform distribution and good colloid stability, measured by hydrodynamic light scattering (DLS). The energy dispersive X-ray spectroscopy (EDS) of NPs confirms the presence of silver and showed that sulfur-rich compounds act as a capping agent being adsorbed on the surface of AgNPs. Antimicrobial tests showed that AgNPs inhibit the bacteria growth, while have no impact on fungi growth. Moreover, tested NPs was characterized by high inhibitory potential of bacteria biofilm formation but also eradication of established biofilms. The cytotoxic effect of the NPs on four mammalian normal and cancer cell lines was tested through the metabolic activity, cell viability and wound-healing assays. Last, but not least, ability to deep penetration of the silver colloid to the root canal was imaged by scanning electron microscopy (SEM) to show its potential as the material for root-end filling.Bartosz SkóraUrszula KrajewskaAnna NowakAndrzej DziedzicAdriana BarylyakMałgorzata Kus-LiśkiewiczNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bartosz Skóra Urszula Krajewska Anna Nowak Andrzej Dziedzic Adriana Barylyak Małgorzata Kus-Liśkiewicz Noncytotoxic silver nanoparticles as a new antimicrobial strategy
description	Abstract Drug-resistance of bacteria is an ongoing problem in hospital treatment. The main mechanism of bacterial virulency in human infections is based on their adhesion ability and biofilm formation. Many approaches have been invented to overcome this problem, i.e. treatment with antibacterial biomolecules, which have some limitations e.g. enzymatic degradation and short shelf stability. Silver nanoparticles (AgNPs) may be alternative to these strategies due to their unique and high antibacterial properties. Herein, we report on yeast Saccharomyces cerevisiae extracellular-based synthesis of AgNPs. Transmission electron microscopy (TEM) revealed the morphology and structure of the metallic nanoparticles, which showed a uniform distribution and good colloid stability, measured by hydrodynamic light scattering (DLS). The energy dispersive X-ray spectroscopy (EDS) of NPs confirms the presence of silver and showed that sulfur-rich compounds act as a capping agent being adsorbed on the surface of AgNPs. Antimicrobial tests showed that AgNPs inhibit the bacteria growth, while have no impact on fungi growth. Moreover, tested NPs was characterized by high inhibitory potential of bacteria biofilm formation but also eradication of established biofilms. The cytotoxic effect of the NPs on four mammalian normal and cancer cell lines was tested through the metabolic activity, cell viability and wound-healing assays. Last, but not least, ability to deep penetration of the silver colloid to the root canal was imaged by scanning electron microscopy (SEM) to show its potential as the material for root-end filling.
format	article
author	Bartosz Skóra Urszula Krajewska Anna Nowak Andrzej Dziedzic Adriana Barylyak Małgorzata Kus-Liśkiewicz
author_facet	Bartosz Skóra Urszula Krajewska Anna Nowak Andrzej Dziedzic Adriana Barylyak Małgorzata Kus-Liśkiewicz
author_sort	Bartosz Skóra
title	Noncytotoxic silver nanoparticles as a new antimicrobial strategy
title_short	Noncytotoxic silver nanoparticles as a new antimicrobial strategy
title_full	Noncytotoxic silver nanoparticles as a new antimicrobial strategy
title_fullStr	Noncytotoxic silver nanoparticles as a new antimicrobial strategy
title_full_unstemmed	Noncytotoxic silver nanoparticles as a new antimicrobial strategy
title_sort	noncytotoxic silver nanoparticles as a new antimicrobial strategy
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b77eb6939a15475ba4c1468ff3a240c0
work_keys_str_mv	AT bartoszskora noncytotoxicsilvernanoparticlesasanewantimicrobialstrategy AT urszulakrajewska noncytotoxicsilvernanoparticlesasanewantimicrobialstrategy AT annanowak noncytotoxicsilvernanoparticlesasanewantimicrobialstrategy AT andrzejdziedzic noncytotoxicsilvernanoparticlesasanewantimicrobialstrategy AT adrianabarylyak noncytotoxicsilvernanoparticlesasanewantimicrobialstrategy AT małgorzatakusliskiewicz noncytotoxicsilvernanoparticlesasanewantimicrobialstrategy
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Noncytotoxic silver nanoparticles as a new antimicrobial strategy

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