Chaihu-Shugan-San administration ameliorates perimenopausal anxiety and depression in rats.

Chaihu-Shugan-San (CSS) is a traditional Chinese herbal formula that is widely used for treating perimenopausal symptoms in China; however, its mechanisms remain unknown. The present study was designed to investigate potential CSS mechanisms in rats with unpredicted chronic mild stress (UCMS) and no...

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Autores principales:	Shujiao Chen, Tetsuya Asakawa, Shanshan Ding, Linghong Liao, Lingyuan Zhang, Jianying Shen, Jie Yu, Kenji Sugiyama, Hiroki Namba, Candong Li
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b7848ec8054249858a37d34a960d183e
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spelling	oai:doaj.org-article:b7848ec8054249858a37d34a960d183e2021-11-18T08:58:00ZChaihu-Shugan-San administration ameliorates perimenopausal anxiety and depression in rats.1932-620310.1371/journal.pone.0072428https://doaj.org/article/b7848ec8054249858a37d34a960d183e2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24015243/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Chaihu-Shugan-San (CSS) is a traditional Chinese herbal formula that is widely used for treating perimenopausal symptoms in China; however, its mechanisms remain unknown. The present study was designed to investigate potential CSS mechanisms in rats with unpredicted chronic mild stress (UCMS) and normally aging rats (52 weeks of age). We performed the sucrose consumption test along with the forced swimming test to confirm depression-like behavior and the open field test (OFT) to confirm anxiety-like behavior in the animals. In addition, we used an enzyme-linked immunosorbent assay to measure serum and hippocampal estradiol (E2) levels and a quantitative real-time polymerase chain reaction to assess hippocampal mRNA levels of estrogen receptors (ERs) α and β as well as G protein-coupled receptor 30 (GPR30). We found that CSS administration resulted in a significant increase in the ratio of hippocampal ERα and ERβ mRNA (ERα/ERβ ratio) in UCMS rats (p<0.001). However, no significant changes were observed in E2 levels, ERα mRNA expression, and GPR30 mRNA expression. In contrast, changes in ERα/ERβ mRNA ratio were sensitively associated with changes in mood states in the animal models. These findings suggest that enhancement of ERα/ERβ ratio may play a role in the pharmacological mechanisms of CSS. Furthermore, this ratio can be employed as a potential index for evaluating mood states in animal models and can be considered as a therapeutic target for perimenopausal anxiety and depression in the future.Shujiao ChenTetsuya AsakawaShanshan DingLinghong LiaoLingyuan ZhangJianying ShenJie YuKenji SugiyamaHiroki NambaCandong LiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e72428 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shujiao Chen Tetsuya Asakawa Shanshan Ding Linghong Liao Lingyuan Zhang Jianying Shen Jie Yu Kenji Sugiyama Hiroki Namba Candong Li Chaihu-Shugan-San administration ameliorates perimenopausal anxiety and depression in rats.
description	Chaihu-Shugan-San (CSS) is a traditional Chinese herbal formula that is widely used for treating perimenopausal symptoms in China; however, its mechanisms remain unknown. The present study was designed to investigate potential CSS mechanisms in rats with unpredicted chronic mild stress (UCMS) and normally aging rats (52 weeks of age). We performed the sucrose consumption test along with the forced swimming test to confirm depression-like behavior and the open field test (OFT) to confirm anxiety-like behavior in the animals. In addition, we used an enzyme-linked immunosorbent assay to measure serum and hippocampal estradiol (E2) levels and a quantitative real-time polymerase chain reaction to assess hippocampal mRNA levels of estrogen receptors (ERs) α and β as well as G protein-coupled receptor 30 (GPR30). We found that CSS administration resulted in a significant increase in the ratio of hippocampal ERα and ERβ mRNA (ERα/ERβ ratio) in UCMS rats (p<0.001). However, no significant changes were observed in E2 levels, ERα mRNA expression, and GPR30 mRNA expression. In contrast, changes in ERα/ERβ mRNA ratio were sensitively associated with changes in mood states in the animal models. These findings suggest that enhancement of ERα/ERβ ratio may play a role in the pharmacological mechanisms of CSS. Furthermore, this ratio can be employed as a potential index for evaluating mood states in animal models and can be considered as a therapeutic target for perimenopausal anxiety and depression in the future.
format	article
author	Shujiao Chen Tetsuya Asakawa Shanshan Ding Linghong Liao Lingyuan Zhang Jianying Shen Jie Yu Kenji Sugiyama Hiroki Namba Candong Li
author_facet	Shujiao Chen Tetsuya Asakawa Shanshan Ding Linghong Liao Lingyuan Zhang Jianying Shen Jie Yu Kenji Sugiyama Hiroki Namba Candong Li
author_sort	Shujiao Chen
title	Chaihu-Shugan-San administration ameliorates perimenopausal anxiety and depression in rats.
title_short	Chaihu-Shugan-San administration ameliorates perimenopausal anxiety and depression in rats.
title_full	Chaihu-Shugan-San administration ameliorates perimenopausal anxiety and depression in rats.
title_fullStr	Chaihu-Shugan-San administration ameliorates perimenopausal anxiety and depression in rats.
title_full_unstemmed	Chaihu-Shugan-San administration ameliorates perimenopausal anxiety and depression in rats.
title_sort	chaihu-shugan-san administration ameliorates perimenopausal anxiety and depression in rats.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/b7848ec8054249858a37d34a960d183e
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Chaihu-Shugan-San administration ameliorates perimenopausal anxiety and depression in rats.

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