Photoacoustic imaging of tumor targeting with riboflavin-functionalized theranostic nanocarriers

Nataliia Beztsinna,1,* Yoanna Tsvetkova,2,* Jithin Jose,3 Boutayna Rhourri-Frih,1 Wa’el Al Rawashdeh,2 Twan Lammers,2 Fabian Kiessling,2 Isabelle Bestel1 1Institute of Chemistry & Biology of Membranes & Nanoobjects (CBMN), UMR 5248, University of Bordeaux, Pessac, France;...

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Autores principales:	Beztsinna N, Tsvetkova Y, Jose J, Rhourri-Frih B, Al Rawashdeh W, Lammers T, Kiessling F, Bestel I
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	photoacoustic imaging nanosystem active targeting riboflavin cancer Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/b7989a49179a497098a8e35ea9a6595c
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spelling	oai:doaj.org-article:b7989a49179a497098a8e35ea9a6595c2021-12-02T02:04:22ZPhotoacoustic imaging of tumor targeting with riboflavin-functionalized theranostic nanocarriers1178-2013https://doaj.org/article/b7989a49179a497098a8e35ea9a6595c2017-05-01T00:00:00Zhttps://www.dovepress.com/photoacoustic-imaging-of-tumor-targeting-with-riboflavin-functionalize-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Nataliia Beztsinna,1,* Yoanna Tsvetkova,2,* Jithin Jose,3 Boutayna Rhourri-Frih,1 Wa’el Al Rawashdeh,2 Twan Lammers,2 Fabian Kiessling,2 Isabelle Bestel1 1Institute of Chemistry & Biology of Membranes & Nanoobjects (CBMN), UMR 5248, University of Bordeaux, Pessac, France; 2Institute for Experimental Molecular Imaging, RWTH Aachen University Clinic, Aachen, Germany; 3Fujifilm VisualSonics BV, Amsterdam, the Netherlands *These authors contributed equally to this work Abstract: Photoacoustic imaging is an emerging method in the molecular imaging field, providing high spatiotemporal resolution and sufficient imaging depths for many clinical applications. Therefore, the aim of this study was to use photoacoustic imaging as a tool to evaluate a riboflavin (RF)-based targeted nanoplatform. RF is internalized by the cells through a specific pathway, and its derivatives were recently shown as promising tumor-targeting vectors for the drug delivery systems. Here, the RF amphiphile synthesized from a PEGylated phospholipid was successfully inserted into a long-circulating liposome formulation labeled with the clinically approved photoacoustic contrast agent – indocyanine green (ICG). The obtained liposomes had a diameter of 124 nm (polydispersity index =0.17) and had a negative zeta potential of –26 mV. Studies in biological phantoms indicated a stable and concentration-dependent photoacoustic signal (Vevo® LAZR) of the ICG-containing RF-functionalized liposomes. In A431 cells, a high uptake of RF-functionalized liposomes was found and could be blocked competitively. First, studies in mice revealed ~3 times higher photoacoustic signal in subcutaneous A431 tumor xenografts (P<0.05) after injection of RF-functionalized liposomes compared to control particles. In this context, the application of a spectral unmixing protocol confirmed the initial quantitative data and improved the localization of liposomes in the tumor. In conclusion, the synthesized RF amphiphile leads to efficient liposomal tumor targeting and can be favorably detected by photoacoustic imaging with a perspective of theranostic applications. Keywords: photoacoustic imaging, long-circulating liposomes, active targeting, riboflavin, indocyanine green Beztsinna NTsvetkova YJose JRhourri-Frih BAl Rawashdeh WLammers TKiessling FBestel IDove Medical Pressarticlephotoacoustic imagingnanosystemactive targetingriboflavincancerMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 3813-3825 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	photoacoustic imaging nanosystem active targeting riboflavin cancer Medicine (General) R5-920
spellingShingle	photoacoustic imaging nanosystem active targeting riboflavin cancer Medicine (General) R5-920 Beztsinna N Tsvetkova Y Jose J Rhourri-Frih B Al Rawashdeh W Lammers T Kiessling F Bestel I Photoacoustic imaging of tumor targeting with riboflavin-functionalized theranostic nanocarriers
description	Nataliia Beztsinna,1,* Yoanna Tsvetkova,2,* Jithin Jose,3 Boutayna Rhourri-Frih,1 Wa’el Al Rawashdeh,2 Twan Lammers,2 Fabian Kiessling,2 Isabelle Bestel1 1Institute of Chemistry & Biology of Membranes & Nanoobjects (CBMN), UMR 5248, University of Bordeaux, Pessac, France; 2Institute for Experimental Molecular Imaging, RWTH Aachen University Clinic, Aachen, Germany; 3Fujifilm VisualSonics BV, Amsterdam, the Netherlands *These authors contributed equally to this work Abstract: Photoacoustic imaging is an emerging method in the molecular imaging field, providing high spatiotemporal resolution and sufficient imaging depths for many clinical applications. Therefore, the aim of this study was to use photoacoustic imaging as a tool to evaluate a riboflavin (RF)-based targeted nanoplatform. RF is internalized by the cells through a specific pathway, and its derivatives were recently shown as promising tumor-targeting vectors for the drug delivery systems. Here, the RF amphiphile synthesized from a PEGylated phospholipid was successfully inserted into a long-circulating liposome formulation labeled with the clinically approved photoacoustic contrast agent – indocyanine green (ICG). The obtained liposomes had a diameter of 124 nm (polydispersity index =0.17) and had a negative zeta potential of –26 mV. Studies in biological phantoms indicated a stable and concentration-dependent photoacoustic signal (Vevo® LAZR) of the ICG-containing RF-functionalized liposomes. In A431 cells, a high uptake of RF-functionalized liposomes was found and could be blocked competitively. First, studies in mice revealed ~3 times higher photoacoustic signal in subcutaneous A431 tumor xenografts (P<0.05) after injection of RF-functionalized liposomes compared to control particles. In this context, the application of a spectral unmixing protocol confirmed the initial quantitative data and improved the localization of liposomes in the tumor. In conclusion, the synthesized RF amphiphile leads to efficient liposomal tumor targeting and can be favorably detected by photoacoustic imaging with a perspective of theranostic applications. Keywords: photoacoustic imaging, long-circulating liposomes, active targeting, riboflavin, indocyanine green
format	article
author	Beztsinna N Tsvetkova Y Jose J Rhourri-Frih B Al Rawashdeh W Lammers T Kiessling F Bestel I
author_facet	Beztsinna N Tsvetkova Y Jose J Rhourri-Frih B Al Rawashdeh W Lammers T Kiessling F Bestel I
author_sort	Beztsinna N
title	Photoacoustic imaging of tumor targeting with riboflavin-functionalized theranostic nanocarriers
title_short	Photoacoustic imaging of tumor targeting with riboflavin-functionalized theranostic nanocarriers
title_full	Photoacoustic imaging of tumor targeting with riboflavin-functionalized theranostic nanocarriers
title_fullStr	Photoacoustic imaging of tumor targeting with riboflavin-functionalized theranostic nanocarriers
title_full_unstemmed	Photoacoustic imaging of tumor targeting with riboflavin-functionalized theranostic nanocarriers
title_sort	photoacoustic imaging of tumor targeting with riboflavin-functionalized theranostic nanocarriers
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/b7989a49179a497098a8e35ea9a6595c
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Photoacoustic imaging of tumor targeting with riboflavin-functionalized theranostic nanocarriers

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