Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2
Here, the authors isolate several nanobodies from a synthetic library that bind the receptor-binding domain (RBD) of SARS-CoV-2 spike protein (S) and neutralize S pseudotyped viruses. Cryo-EM structure of Spike with one nanobody and further biophysical analysis shows competition with ACE2 binding.
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Nature Portfolio
2020
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oai:doaj.org-article:b79a9adc234e460f9baa5ef6524bc6bf2021-12-02T16:49:30ZSelection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-210.1038/s41467-020-19204-y2041-1723https://doaj.org/article/b79a9adc234e460f9baa5ef6524bc6bf2020-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-19204-yhttps://doaj.org/toc/2041-1723Here, the authors isolate several nanobodies from a synthetic library that bind the receptor-binding domain (RBD) of SARS-CoV-2 spike protein (S) and neutralize S pseudotyped viruses. Cryo-EM structure of Spike with one nanobody and further biophysical analysis shows competition with ACE2 binding.Tânia F. CustódioHrishikesh DasDaniel J. ShewardLeo HankeSamuel PazickyJoanna PieprzykMichèle SorgenfreiMartin A. SchroerAndrey Yu. GruzinovCy M. JeffriesMelissa A. GraewertDmitri I. SvergunNikolay DobrevKim RemansMarkus A. SeegerGerald M. McInerneyBen MurrellB. Martin HällbergChristian LöwNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-11 (2020) |
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| topic |
Science Q |
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Science Q Tânia F. Custódio Hrishikesh Das Daniel J. Sheward Leo Hanke Samuel Pazicky Joanna Pieprzyk Michèle Sorgenfrei Martin A. Schroer Andrey Yu. Gruzinov Cy M. Jeffries Melissa A. Graewert Dmitri I. Svergun Nikolay Dobrev Kim Remans Markus A. Seeger Gerald M. McInerney Ben Murrell B. Martin Hällberg Christian Löw Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2 |
| description |
Here, the authors isolate several nanobodies from a synthetic library that bind the receptor-binding domain (RBD) of SARS-CoV-2 spike protein (S) and neutralize S pseudotyped viruses. Cryo-EM structure of Spike with one nanobody and further biophysical analysis shows competition with ACE2 binding. |
| format |
article |
| author |
Tânia F. Custódio Hrishikesh Das Daniel J. Sheward Leo Hanke Samuel Pazicky Joanna Pieprzyk Michèle Sorgenfrei Martin A. Schroer Andrey Yu. Gruzinov Cy M. Jeffries Melissa A. Graewert Dmitri I. Svergun Nikolay Dobrev Kim Remans Markus A. Seeger Gerald M. McInerney Ben Murrell B. Martin Hällberg Christian Löw |
| author_facet |
Tânia F. Custódio Hrishikesh Das Daniel J. Sheward Leo Hanke Samuel Pazicky Joanna Pieprzyk Michèle Sorgenfrei Martin A. Schroer Andrey Yu. Gruzinov Cy M. Jeffries Melissa A. Graewert Dmitri I. Svergun Nikolay Dobrev Kim Remans Markus A. Seeger Gerald M. McInerney Ben Murrell B. Martin Hällberg Christian Löw |
| author_sort |
Tânia F. Custódio |
| title |
Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2 |
| title_short |
Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2 |
| title_full |
Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2 |
| title_fullStr |
Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2 |
| title_full_unstemmed |
Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2 |
| title_sort |
selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize sars-cov-2 |
| publisher |
Nature Portfolio |
| publishDate |
2020 |
| url |
https://doaj.org/article/b79a9adc234e460f9baa5ef6524bc6bf |
| work_keys_str_mv |
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