Functional analysis of the cotton CLE polypeptide signaling gene family in plant growth and development

Abstract The CLAVATA3 (CLV3)/EMBRYO SURROUNDING REGION (ESR)–RELATED (CLE) gene family encodes a large number of polypeptide signaling molecules involved in the regulation of shoot apical meristem division and root and vascular bundle development in a variety of plants. CLE family genes encode impor...

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Autores principales:	Ke Wan, Kening Lu, Mengtao Gao, Ting Zhao, Yuxin He, Dong-Lei Yang, Xiaoyuan Tao, Guosheng Xiong, Xueying Guan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b79c431ac4f040daaf39c0d8677b0349
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spelling	oai:doaj.org-article:b79c431ac4f040daaf39c0d8677b03492021-12-02T11:35:52ZFunctional analysis of the cotton CLE polypeptide signaling gene family in plant growth and development10.1038/s41598-021-84312-82045-2322https://doaj.org/article/b79c431ac4f040daaf39c0d8677b03492021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84312-8https://doaj.org/toc/2045-2322Abstract The CLAVATA3 (CLV3)/EMBRYO SURROUNDING REGION (ESR)–RELATED (CLE) gene family encodes a large number of polypeptide signaling molecules involved in the regulation of shoot apical meristem division and root and vascular bundle development in a variety of plants. CLE family genes encode important short peptide hormones; however, the functions of these signaling polypeptides in cotton remain largely unknown. In the current work, we studied the effects of the CLE family genes on growth and development in cotton. Based on the presence of a conserved CLE motif of 13 amino acids, 93 genes were characterized as GhCLE gene family members, and these were subcategorized into 7 groups. A preliminary analysis of the cotton CLE gene family indicated that the activity of its members tends to be conserved in terms of both the 13-residue conserved domain at the C-terminus and their subcellular localization pattern. Among the 14 tested genes, the ectopic overexpression of GhCLE5::GFP partially mimicked the phenotype of the clv3 mutant in Arabidopsis. GhCLE5 could affect the endogenous CLV3 in binding to the receptor complex, comprised of CLV1, CLV2, and CRN, in the yeast two-hybrid assay and split-luciferase assay. Silencing GhCLE5 in cotton caused a short seedling phenotype. Therefore, we concluded that the cotton GhCLE gene family is functionally conserved in apical shoot development regulation. These results indicate that CLE also plays roles in cotton development as a short peptide hormone.Ke WanKening LuMengtao GaoTing ZhaoYuxin HeDong-Lei YangXiaoyuan TaoGuosheng XiongXueying GuanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ke Wan Kening Lu Mengtao Gao Ting Zhao Yuxin He Dong-Lei Yang Xiaoyuan Tao Guosheng Xiong Xueying Guan Functional analysis of the cotton CLE polypeptide signaling gene family in plant growth and development
description	Abstract The CLAVATA3 (CLV3)/EMBRYO SURROUNDING REGION (ESR)–RELATED (CLE) gene family encodes a large number of polypeptide signaling molecules involved in the regulation of shoot apical meristem division and root and vascular bundle development in a variety of plants. CLE family genes encode important short peptide hormones; however, the functions of these signaling polypeptides in cotton remain largely unknown. In the current work, we studied the effects of the CLE family genes on growth and development in cotton. Based on the presence of a conserved CLE motif of 13 amino acids, 93 genes were characterized as GhCLE gene family members, and these were subcategorized into 7 groups. A preliminary analysis of the cotton CLE gene family indicated that the activity of its members tends to be conserved in terms of both the 13-residue conserved domain at the C-terminus and their subcellular localization pattern. Among the 14 tested genes, the ectopic overexpression of GhCLE5::GFP partially mimicked the phenotype of the clv3 mutant in Arabidopsis. GhCLE5 could affect the endogenous CLV3 in binding to the receptor complex, comprised of CLV1, CLV2, and CRN, in the yeast two-hybrid assay and split-luciferase assay. Silencing GhCLE5 in cotton caused a short seedling phenotype. Therefore, we concluded that the cotton GhCLE gene family is functionally conserved in apical shoot development regulation. These results indicate that CLE also plays roles in cotton development as a short peptide hormone.
format	article
author	Ke Wan Kening Lu Mengtao Gao Ting Zhao Yuxin He Dong-Lei Yang Xiaoyuan Tao Guosheng Xiong Xueying Guan
author_facet	Ke Wan Kening Lu Mengtao Gao Ting Zhao Yuxin He Dong-Lei Yang Xiaoyuan Tao Guosheng Xiong Xueying Guan
author_sort	Ke Wan
title	Functional analysis of the cotton CLE polypeptide signaling gene family in plant growth and development
title_short	Functional analysis of the cotton CLE polypeptide signaling gene family in plant growth and development
title_full	Functional analysis of the cotton CLE polypeptide signaling gene family in plant growth and development
title_fullStr	Functional analysis of the cotton CLE polypeptide signaling gene family in plant growth and development
title_full_unstemmed	Functional analysis of the cotton CLE polypeptide signaling gene family in plant growth and development
title_sort	functional analysis of the cotton cle polypeptide signaling gene family in plant growth and development
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b79c431ac4f040daaf39c0d8677b0349
work_keys_str_mv	AT kewan functionalanalysisofthecottonclepolypeptidesignalinggenefamilyinplantgrowthanddevelopment AT keninglu functionalanalysisofthecottonclepolypeptidesignalinggenefamilyinplantgrowthanddevelopment AT mengtaogao functionalanalysisofthecottonclepolypeptidesignalinggenefamilyinplantgrowthanddevelopment AT tingzhao functionalanalysisofthecottonclepolypeptidesignalinggenefamilyinplantgrowthanddevelopment AT yuxinhe functionalanalysisofthecottonclepolypeptidesignalinggenefamilyinplantgrowthanddevelopment AT dongleiyang functionalanalysisofthecottonclepolypeptidesignalinggenefamilyinplantgrowthanddevelopment AT xiaoyuantao functionalanalysisofthecottonclepolypeptidesignalinggenefamilyinplantgrowthanddevelopment AT guoshengxiong functionalanalysisofthecottonclepolypeptidesignalinggenefamilyinplantgrowthanddevelopment AT xueyingguan functionalanalysisofthecottonclepolypeptidesignalinggenefamilyinplantgrowthanddevelopment
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Functional analysis of the cotton CLE polypeptide signaling gene family in plant growth and development

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