Responses of Enterococcus faecalis resistance and cytolysin up-regulation to nutrients in constructed mesocosms

Spreading of multidrug-resistant bacteria has become a growing and significant threat to environmental and public health. Our understanding of environmental variables such as nutrients which contribute to the dissemination of antibiotic resistance and pathogenesis is still limited. In this study, we...

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Autores principales:	Liaqat Ali, Muhammad Mustafa, Zheng Run Xiao, Waqar Islam, Ulfat Ara, Muhammad Ajmal, Khalid Ali Khan, Hamed A. Ghramh, Daojin Yu
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Antibiotic resistance Aquatic environment Enterococcus faecalis Nitrate and Phosphate ions Nutrient pollution Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/b7a61c95dbc4476ba865f731d941c35e
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spelling	oai:doaj.org-article:b7a61c95dbc4476ba865f731d941c35e2021-11-16T04:09:09ZResponses of Enterococcus faecalis resistance and cytolysin up-regulation to nutrients in constructed mesocosms1018-364710.1016/j.jksus.2021.101680https://doaj.org/article/b7a61c95dbc4476ba865f731d941c35e2022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1018364721003426https://doaj.org/toc/1018-3647Spreading of multidrug-resistant bacteria has become a growing and significant threat to environmental and public health. Our understanding of environmental variables such as nutrients which contribute to the dissemination of antibiotic resistance and pathogenesis is still limited. In this study, we operated outdoor mesocosm experiments to evaluate how nitrate and phosphate ions with different concentrations influence on an adaptation of Enterococcus faecalis to aquatic environments. E. faecalis were isolated from the mesocosms at 8 sampling events for 96 days to evaluate biofilm production, quorum-sensing-related genes expression, and sensitivity to oxytetracycline, erythromycin, ciprofloxacin, ampicillin, vancomycin, and chloramphenicol. Quantitative real-time PCR was used to compare mRNA levels of E. faecalis quorum-sensing-related genes. E. faecalis isolates exhibited resistance to oxytetracycline, ampicillin, and ciprofloxacin, respectively. We observed that the biofilm production of E. faecalis isolates on day 60 and 96 was significantly increased (p < 0.01). The expressions of quorum-sensing-related genes were significantly up-regulated (∼tenfold) at the transcriptional level in nutrient-enriched treatments. Our findings indicate that nitrate and phosphate ions facilitate resistance to commonly used antibiotics, increase biofilm production and intra-species communication, which could be a major reviving strategy of E. faecalis in the aquatic environments.Liaqat AliMuhammad MustafaZheng Run XiaoWaqar IslamUlfat AraMuhammad AjmalKhalid Ali KhanHamed A. GhramhDaojin YuElsevierarticleAntibiotic resistanceAquatic environmentEnterococcus faecalisNitrate and Phosphate ionsNutrient pollutionScience (General)Q1-390ENJournal of King Saud University: Science, Vol 34, Iss 1, Pp 101680- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Antibiotic resistance Aquatic environment Enterococcus faecalis Nitrate and Phosphate ions Nutrient pollution Science (General) Q1-390
spellingShingle	Antibiotic resistance Aquatic environment Enterococcus faecalis Nitrate and Phosphate ions Nutrient pollution Science (General) Q1-390 Liaqat Ali Muhammad Mustafa Zheng Run Xiao Waqar Islam Ulfat Ara Muhammad Ajmal Khalid Ali Khan Hamed A. Ghramh Daojin Yu Responses of Enterococcus faecalis resistance and cytolysin up-regulation to nutrients in constructed mesocosms
description	Spreading of multidrug-resistant bacteria has become a growing and significant threat to environmental and public health. Our understanding of environmental variables such as nutrients which contribute to the dissemination of antibiotic resistance and pathogenesis is still limited. In this study, we operated outdoor mesocosm experiments to evaluate how nitrate and phosphate ions with different concentrations influence on an adaptation of Enterococcus faecalis to aquatic environments. E. faecalis were isolated from the mesocosms at 8 sampling events for 96 days to evaluate biofilm production, quorum-sensing-related genes expression, and sensitivity to oxytetracycline, erythromycin, ciprofloxacin, ampicillin, vancomycin, and chloramphenicol. Quantitative real-time PCR was used to compare mRNA levels of E. faecalis quorum-sensing-related genes. E. faecalis isolates exhibited resistance to oxytetracycline, ampicillin, and ciprofloxacin, respectively. We observed that the biofilm production of E. faecalis isolates on day 60 and 96 was significantly increased (p < 0.01). The expressions of quorum-sensing-related genes were significantly up-regulated (∼tenfold) at the transcriptional level in nutrient-enriched treatments. Our findings indicate that nitrate and phosphate ions facilitate resistance to commonly used antibiotics, increase biofilm production and intra-species communication, which could be a major reviving strategy of E. faecalis in the aquatic environments.
format	article
author	Liaqat Ali Muhammad Mustafa Zheng Run Xiao Waqar Islam Ulfat Ara Muhammad Ajmal Khalid Ali Khan Hamed A. Ghramh Daojin Yu
author_facet	Liaqat Ali Muhammad Mustafa Zheng Run Xiao Waqar Islam Ulfat Ara Muhammad Ajmal Khalid Ali Khan Hamed A. Ghramh Daojin Yu
author_sort	Liaqat Ali
title	Responses of Enterococcus faecalis resistance and cytolysin up-regulation to nutrients in constructed mesocosms
title_short	Responses of Enterococcus faecalis resistance and cytolysin up-regulation to nutrients in constructed mesocosms
title_full	Responses of Enterococcus faecalis resistance and cytolysin up-regulation to nutrients in constructed mesocosms
title_fullStr	Responses of Enterococcus faecalis resistance and cytolysin up-regulation to nutrients in constructed mesocosms
title_full_unstemmed	Responses of Enterococcus faecalis resistance and cytolysin up-regulation to nutrients in constructed mesocosms
title_sort	responses of enterococcus faecalis resistance and cytolysin up-regulation to nutrients in constructed mesocosms
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/b7a61c95dbc4476ba865f731d941c35e
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Responses of Enterococcus faecalis resistance and cytolysin up-regulation to nutrients in constructed mesocosms

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