Non-coding RNA suppresses FUS aggregation caused by mechanistic shear stress on pipetting in a sequence-dependent manner

Abstract Fused in sarcoma/translocated in liposarcoma (FUS/TLS) is a multitasking RNA/DNA binding protein. FUS aggregation is implicated in various neurodegenerative diseases. RNA was suggested to modulate phase transition of FUS. Here, we found that FUS transforms into the amorphous aggregation sta...

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Autores principales:	Nesreen Hamad, Ryoma Yoneda, Masatomo So, Riki Kurokawa, Takashi Nagata, Masato Katahira
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b7b2712f3e574bf6929132ce72be59a8
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spelling	oai:doaj.org-article:b7b2712f3e574bf6929132ce72be59a82021-12-02T15:37:58ZNon-coding RNA suppresses FUS aggregation caused by mechanistic shear stress on pipetting in a sequence-dependent manner10.1038/s41598-021-89075-w2045-2322https://doaj.org/article/b7b2712f3e574bf6929132ce72be59a82021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89075-whttps://doaj.org/toc/2045-2322Abstract Fused in sarcoma/translocated in liposarcoma (FUS/TLS) is a multitasking RNA/DNA binding protein. FUS aggregation is implicated in various neurodegenerative diseases. RNA was suggested to modulate phase transition of FUS. Here, we found that FUS transforms into the amorphous aggregation state as an instant response to the shear stress caused by usual pipetting even at a low FUS concentration, 100 nM. It was revealed that non-coding RNA can suppress the transformation of FUS into aggregates. The suppressive effect of RNA on FUS aggregation is sequence-dependent. These results suggested that the non-coding RNA could be a prospective suppressor of FUS aggregation caused by mechanistic stress in cells. Our finding might pave the way for more research on the role of RNAs as aggregation inhibitors, which could facilitate the development of therapies for neurodegenerative diseases.Nesreen HamadRyoma YonedaMasatomo SoRiki KurokawaTakashi NagataMasato KatahiraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nesreen Hamad Ryoma Yoneda Masatomo So Riki Kurokawa Takashi Nagata Masato Katahira Non-coding RNA suppresses FUS aggregation caused by mechanistic shear stress on pipetting in a sequence-dependent manner
description	Abstract Fused in sarcoma/translocated in liposarcoma (FUS/TLS) is a multitasking RNA/DNA binding protein. FUS aggregation is implicated in various neurodegenerative diseases. RNA was suggested to modulate phase transition of FUS. Here, we found that FUS transforms into the amorphous aggregation state as an instant response to the shear stress caused by usual pipetting even at a low FUS concentration, 100 nM. It was revealed that non-coding RNA can suppress the transformation of FUS into aggregates. The suppressive effect of RNA on FUS aggregation is sequence-dependent. These results suggested that the non-coding RNA could be a prospective suppressor of FUS aggregation caused by mechanistic stress in cells. Our finding might pave the way for more research on the role of RNAs as aggregation inhibitors, which could facilitate the development of therapies for neurodegenerative diseases.
format	article
author	Nesreen Hamad Ryoma Yoneda Masatomo So Riki Kurokawa Takashi Nagata Masato Katahira
author_facet	Nesreen Hamad Ryoma Yoneda Masatomo So Riki Kurokawa Takashi Nagata Masato Katahira
author_sort	Nesreen Hamad
title	Non-coding RNA suppresses FUS aggregation caused by mechanistic shear stress on pipetting in a sequence-dependent manner
title_short	Non-coding RNA suppresses FUS aggregation caused by mechanistic shear stress on pipetting in a sequence-dependent manner
title_full	Non-coding RNA suppresses FUS aggregation caused by mechanistic shear stress on pipetting in a sequence-dependent manner
title_fullStr	Non-coding RNA suppresses FUS aggregation caused by mechanistic shear stress on pipetting in a sequence-dependent manner
title_full_unstemmed	Non-coding RNA suppresses FUS aggregation caused by mechanistic shear stress on pipetting in a sequence-dependent manner
title_sort	non-coding rna suppresses fus aggregation caused by mechanistic shear stress on pipetting in a sequence-dependent manner
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b7b2712f3e574bf6929132ce72be59a8
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Non-coding RNA suppresses FUS aggregation caused by mechanistic shear stress on pipetting in a sequence-dependent manner

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