Dietary supplementation with probiotics improves hematopoiesis in malnourished mice.

<h4>Background</h4>Lactobacillus rhamnosus CRL1505 (Lr) administered during the repletion of immunocompromised-malnourished mice improves the resistance against intestinal and respiratory infections. This effect is associated with an increase in the number and functionality of immune cel...

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Autores principales:	Susana Salva, María Cecilia Merino, Graciela Agüero, Adriana Gruppi, Susana Alvarez
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b7b37e5d9f2b4a43ba65b26d395a5b61
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spelling	oai:doaj.org-article:b7b37e5d9f2b4a43ba65b26d395a5b612021-11-18T07:28:39ZDietary supplementation with probiotics improves hematopoiesis in malnourished mice.1932-620310.1371/journal.pone.0031171https://doaj.org/article/b7b37e5d9f2b4a43ba65b26d395a5b612012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22347448/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Lactobacillus rhamnosus CRL1505 (Lr) administered during the repletion of immunocompromised-malnourished mice improves the resistance against intestinal and respiratory infections. This effect is associated with an increase in the number and functionality of immune cells, indicating that Lr could have some influence on myeloid and lymphoid cell production and maturation.<h4>Objective</h4>This study analyzed the extent of the damage caused by malnutrition on myeloid and lymphoid cell development in the spleen and bone marrow (BM). We also evaluated the impact of immunobiotics on the recovery of hematopoiesis affected in malnourished mice.<h4>Methods</h4>Protein malnourished mice were fed on a balanced conventional diet for 7 or 14 consecutive d with or without supplemental Lr or fermented goat's milk (FGM). Malnourished mice and well-nourished mice were used as controls. Histological and flow cytometry studies were carried out in BM and spleen to study myeloid and lymphoid cells.<h4>Results</h4>Malnutrition induced quantitative alterations in spleen B and T cells; however, no alteration was observed in the ability of splenic B cells to produce immunoglobulins after challenge with LPS or CpG. The analysis of BM B cell subsets based on B220, CD24, IgM and IgD expression showed that malnutrition affected B cell development. In addition, BM myeloid cells decreased in malnourished mice. On the contrary, protein deprivation increased BM T cell number. These alterations were reverted with Lr or FGM repletion treatments since normal numbers of BM myeloid, T and B cells were observed in these groups.<h4>Conclusions</h4>Protein malnutrition significantly alters B cell development in BM. The treatment of malnourished mice with L. rhamnosus CRL1505 was able to induce a recovery of B cells that would explain its ability to increase immunity against infections. This work highlights the possibility of using immunobiotics to accelerate the recovery of lymphopoyesis in immunocompromised-malnourished hosts.Susana SalvaMaría Cecilia MerinoGraciela AgüeroAdriana GruppiSusana AlvarezPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 2, p e31171 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Susana Salva María Cecilia Merino Graciela Agüero Adriana Gruppi Susana Alvarez Dietary supplementation with probiotics improves hematopoiesis in malnourished mice.
description	<h4>Background</h4>Lactobacillus rhamnosus CRL1505 (Lr) administered during the repletion of immunocompromised-malnourished mice improves the resistance against intestinal and respiratory infections. This effect is associated with an increase in the number and functionality of immune cells, indicating that Lr could have some influence on myeloid and lymphoid cell production and maturation.<h4>Objective</h4>This study analyzed the extent of the damage caused by malnutrition on myeloid and lymphoid cell development in the spleen and bone marrow (BM). We also evaluated the impact of immunobiotics on the recovery of hematopoiesis affected in malnourished mice.<h4>Methods</h4>Protein malnourished mice were fed on a balanced conventional diet for 7 or 14 consecutive d with or without supplemental Lr or fermented goat's milk (FGM). Malnourished mice and well-nourished mice were used as controls. Histological and flow cytometry studies were carried out in BM and spleen to study myeloid and lymphoid cells.<h4>Results</h4>Malnutrition induced quantitative alterations in spleen B and T cells; however, no alteration was observed in the ability of splenic B cells to produce immunoglobulins after challenge with LPS or CpG. The analysis of BM B cell subsets based on B220, CD24, IgM and IgD expression showed that malnutrition affected B cell development. In addition, BM myeloid cells decreased in malnourished mice. On the contrary, protein deprivation increased BM T cell number. These alterations were reverted with Lr or FGM repletion treatments since normal numbers of BM myeloid, T and B cells were observed in these groups.<h4>Conclusions</h4>Protein malnutrition significantly alters B cell development in BM. The treatment of malnourished mice with L. rhamnosus CRL1505 was able to induce a recovery of B cells that would explain its ability to increase immunity against infections. This work highlights the possibility of using immunobiotics to accelerate the recovery of lymphopoyesis in immunocompromised-malnourished hosts.
format	article
author	Susana Salva María Cecilia Merino Graciela Agüero Adriana Gruppi Susana Alvarez
author_facet	Susana Salva María Cecilia Merino Graciela Agüero Adriana Gruppi Susana Alvarez
author_sort	Susana Salva
title	Dietary supplementation with probiotics improves hematopoiesis in malnourished mice.
title_short	Dietary supplementation with probiotics improves hematopoiesis in malnourished mice.
title_full	Dietary supplementation with probiotics improves hematopoiesis in malnourished mice.
title_fullStr	Dietary supplementation with probiotics improves hematopoiesis in malnourished mice.
title_full_unstemmed	Dietary supplementation with probiotics improves hematopoiesis in malnourished mice.
title_sort	dietary supplementation with probiotics improves hematopoiesis in malnourished mice.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/b7b37e5d9f2b4a43ba65b26d395a5b61
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Dietary supplementation with probiotics improves hematopoiesis in malnourished mice.

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