Comparative study of porous hydroxyapatite/chitosan and whitlockite/chitosan scaffolds for bone regeneration in calvarial defects

Ding Zhou,1,* Chao Qi,2,* Yi-Xuan Chen,1 Ying-Jie Zhu,2 Tuan-Wei Sun,2 Feng Chen,2 Chang-Qing Zhang1 1Department of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai Jiaotong University, 2State Key Laboratory of High Performance Ceramics and Su...

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Autores principales:	Zhou D, Qi C, Chen Y, Zhu YJ, Sun T, Chen F, Zhang C
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	hydroxyapatite whitlockite chitosan osteogenic differentiation rat critical calvaria defect tissue engineering Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/b7b532dd598946eca19899d9b350f2c2
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spelling	oai:doaj.org-article:b7b532dd598946eca19899d9b350f2c22021-12-02T05:02:07ZComparative study of porous hydroxyapatite/chitosan and whitlockite/chitosan scaffolds for bone regeneration in calvarial defects1178-2013https://doaj.org/article/b7b532dd598946eca19899d9b350f2c22017-04-01T00:00:00Zhttps://www.dovepress.com/comparative-study-of-porous-hydroxyapatitechitosan-and-whitlockitechit-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Ding Zhou,1,* Chao Qi,2,* Yi-Xuan Chen,1 Ying-Jie Zhu,2 Tuan-Wei Sun,2 Feng Chen,2 Chang-Qing Zhang1 1Department of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai Jiaotong University, 2State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai, People’s Republic of China *These authors contributed equally to this work Abstract: Hydroxyapatite (HAP; Ca10(PO4)6(OH)2) and whitlockite (WH; Ca18Mg2(HPO4)2(PO4)12) are widely utilized in bone repair because they are the main components of hard tissues such as bones and teeth. In this paper, we synthesized HAP and WH hollow microspheres by using creatine phosphate disodium salt as an organic phosphorus source in aqueous solution through microwave-assisted hydrothermal method. Then, we prepared HAP/chitosan and WH/chitosan composite membranes to evaluate their biocompatibility in vitro and prepared porous HAP/chitosan and WH/chitosan scaffolds by freeze drying to compare their effects on bone regeneration in calvarial defects in a rat model. The experimental results indicated that the WH/chitosan composite membrane had a better biocompatibility, enhancing proliferation and osteogenic differentiation ability of human mesenchymal stem cells than HAP/chitosan. Moreover, the porous WH/chitosan scaffold can significantly promote bone regeneration in calvarial defects, and thus it is more promising for applications in tissue engineering such as calvarial repair compared to porous HAP/chitosan scaffold. Keywords: hydroxyapatite, whitlockite, chitosan, osteogenic differentiation, rat critical calvarial defect, tissue engineeringZhou DQi CChen YZhu YJSun TChen FZhang CDove Medical Pressarticlehydroxyapatitewhitlockitechitosanosteogenic differentiationrat critical calvaria defecttissue engineeringMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 2673-2687 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	hydroxyapatite whitlockite chitosan osteogenic differentiation rat critical calvaria defect tissue engineering Medicine (General) R5-920
spellingShingle	hydroxyapatite whitlockite chitosan osteogenic differentiation rat critical calvaria defect tissue engineering Medicine (General) R5-920 Zhou D Qi C Chen Y Zhu YJ Sun T Chen F Zhang C Comparative study of porous hydroxyapatite/chitosan and whitlockite/chitosan scaffolds for bone regeneration in calvarial defects
description	Ding Zhou,1,* Chao Qi,2,* Yi-Xuan Chen,1 Ying-Jie Zhu,2 Tuan-Wei Sun,2 Feng Chen,2 Chang-Qing Zhang1 1Department of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai Jiaotong University, 2State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai, People’s Republic of China *These authors contributed equally to this work Abstract: Hydroxyapatite (HAP; Ca10(PO4)6(OH)2) and whitlockite (WH; Ca18Mg2(HPO4)2(PO4)12) are widely utilized in bone repair because they are the main components of hard tissues such as bones and teeth. In this paper, we synthesized HAP and WH hollow microspheres by using creatine phosphate disodium salt as an organic phosphorus source in aqueous solution through microwave-assisted hydrothermal method. Then, we prepared HAP/chitosan and WH/chitosan composite membranes to evaluate their biocompatibility in vitro and prepared porous HAP/chitosan and WH/chitosan scaffolds by freeze drying to compare their effects on bone regeneration in calvarial defects in a rat model. The experimental results indicated that the WH/chitosan composite membrane had a better biocompatibility, enhancing proliferation and osteogenic differentiation ability of human mesenchymal stem cells than HAP/chitosan. Moreover, the porous WH/chitosan scaffold can significantly promote bone regeneration in calvarial defects, and thus it is more promising for applications in tissue engineering such as calvarial repair compared to porous HAP/chitosan scaffold. Keywords: hydroxyapatite, whitlockite, chitosan, osteogenic differentiation, rat critical calvarial defect, tissue engineering
format	article
author	Zhou D Qi C Chen Y Zhu YJ Sun T Chen F Zhang C
author_facet	Zhou D Qi C Chen Y Zhu YJ Sun T Chen F Zhang C
author_sort	Zhou D
title	Comparative study of porous hydroxyapatite/chitosan and whitlockite/chitosan scaffolds for bone regeneration in calvarial defects
title_short	Comparative study of porous hydroxyapatite/chitosan and whitlockite/chitosan scaffolds for bone regeneration in calvarial defects
title_full	Comparative study of porous hydroxyapatite/chitosan and whitlockite/chitosan scaffolds for bone regeneration in calvarial defects
title_fullStr	Comparative study of porous hydroxyapatite/chitosan and whitlockite/chitosan scaffolds for bone regeneration in calvarial defects
title_full_unstemmed	Comparative study of porous hydroxyapatite/chitosan and whitlockite/chitosan scaffolds for bone regeneration in calvarial defects
title_sort	comparative study of porous hydroxyapatite/chitosan and whitlockite/chitosan scaffolds for bone regeneration in calvarial defects
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/b7b532dd598946eca19899d9b350f2c2
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Comparative study of porous hydroxyapatite/chitosan and whitlockite/chitosan scaffolds for bone regeneration in calvarial defects

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