North American Triple Reassortant and Eurasian H1N1 Swine Influenza Viruses Do Not Readily Reassort to Generate a 2009 Pandemic H1N1-Like Virus

ABSTRACT The 2009 pandemic H1N1 virus (pH1N1) was derived through reassortment of North American triple reassortant and Eurasian avian-like swine influenza viruses (SIVs). To date, when, how and where the pH1N1 arose is not understood. To investigate viral reassortment, we coinfected cell cultures a...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wenjun Ma, Qinfang Liu, Chuanling Qiao, Gustavo del Real, Adolfo García-Sastre, Richard J. Webby, Jürgen A. Richt
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2014
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/b7b5d9163fbf4a7aa9357db409fad9c3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b7b5d9163fbf4a7aa9357db409fad9c3
record_format	dspace
spelling	oai:doaj.org-article:b7b5d9163fbf4a7aa9357db409fad9c32021-11-15T15:45:13ZNorth American Triple Reassortant and Eurasian H1N1 Swine Influenza Viruses Do Not Readily Reassort to Generate a 2009 Pandemic H1N1-Like Virus10.1128/mBio.00919-132150-7511https://doaj.org/article/b7b5d9163fbf4a7aa9357db409fad9c32014-05-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00919-13https://doaj.org/toc/2150-7511ABSTRACT The 2009 pandemic H1N1 virus (pH1N1) was derived through reassortment of North American triple reassortant and Eurasian avian-like swine influenza viruses (SIVs). To date, when, how and where the pH1N1 arose is not understood. To investigate viral reassortment, we coinfected cell cultures and a group of pigs with or without preexisting immunity with a Eurasian H1N1 virus, A/Swine/Spain/53207/2004 (SP04), and a North American triple reassortant H1N1 virus, A/Swine/Kansas/77778/2007 (KS07). The infected pigs were cohoused with one or two groups of contact animals to investigate viral transmission. In coinfected MDCK or PK15 continuous cell lines with KS07 and SP04 viruses, more than 20 different reassortant viruses were found. In pigs without or with preexisting immunity (immunized with commercial inactivated swine influenza vaccines) and coinfected with both viruses, six or seven reassortant viruses, as well as the parental viruses, were identified in bronchoalveolar lavage fluid samples from the lungs. Interestingly, only one or two viruses transmitted to and were detected in contact animals. No reassortant containing a gene constellation similar to that of pH1N1 virus was found in either coinfected cells or pigs, indicating that the reassortment event that resulted in the generation of this virus is a rare event that likely involved specific viral strains and/or a favorable, not-yet-understood environment. IMPORTANCE The 2009 pandemic-like H1N1 virus could not be reproduced either in cell cultures or in pigs coinfected with North American triple reassortant H1N1 and Eurasian H1N1 swine influenza viruses. This finding suggests that the generation of the 2009 pandemic H1N1 virus by reassortment was a rare event that likely involved specific viral strains and unknown factors. Different reassortant viruses were detected in coinfected pigs with and without preexisting immunity, indicating that host immunity plays a relevant role in driving viral reassortment of influenza A virus.Wenjun MaQinfang LiuChuanling QiaoGustavo del RealAdolfo García-SastreRichard J. WebbyJürgen A. RichtAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 5, Iss 2 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Wenjun Ma Qinfang Liu Chuanling Qiao Gustavo del Real Adolfo García-Sastre Richard J. Webby Jürgen A. Richt North American Triple Reassortant and Eurasian H1N1 Swine Influenza Viruses Do Not Readily Reassort to Generate a 2009 Pandemic H1N1-Like Virus
description	ABSTRACT The 2009 pandemic H1N1 virus (pH1N1) was derived through reassortment of North American triple reassortant and Eurasian avian-like swine influenza viruses (SIVs). To date, when, how and where the pH1N1 arose is not understood. To investigate viral reassortment, we coinfected cell cultures and a group of pigs with or without preexisting immunity with a Eurasian H1N1 virus, A/Swine/Spain/53207/2004 (SP04), and a North American triple reassortant H1N1 virus, A/Swine/Kansas/77778/2007 (KS07). The infected pigs were cohoused with one or two groups of contact animals to investigate viral transmission. In coinfected MDCK or PK15 continuous cell lines with KS07 and SP04 viruses, more than 20 different reassortant viruses were found. In pigs without or with preexisting immunity (immunized with commercial inactivated swine influenza vaccines) and coinfected with both viruses, six or seven reassortant viruses, as well as the parental viruses, were identified in bronchoalveolar lavage fluid samples from the lungs. Interestingly, only one or two viruses transmitted to and were detected in contact animals. No reassortant containing a gene constellation similar to that of pH1N1 virus was found in either coinfected cells or pigs, indicating that the reassortment event that resulted in the generation of this virus is a rare event that likely involved specific viral strains and/or a favorable, not-yet-understood environment. IMPORTANCE The 2009 pandemic-like H1N1 virus could not be reproduced either in cell cultures or in pigs coinfected with North American triple reassortant H1N1 and Eurasian H1N1 swine influenza viruses. This finding suggests that the generation of the 2009 pandemic H1N1 virus by reassortment was a rare event that likely involved specific viral strains and unknown factors. Different reassortant viruses were detected in coinfected pigs with and without preexisting immunity, indicating that host immunity plays a relevant role in driving viral reassortment of influenza A virus.
format	article
author	Wenjun Ma Qinfang Liu Chuanling Qiao Gustavo del Real Adolfo García-Sastre Richard J. Webby Jürgen A. Richt
author_facet	Wenjun Ma Qinfang Liu Chuanling Qiao Gustavo del Real Adolfo García-Sastre Richard J. Webby Jürgen A. Richt
author_sort	Wenjun Ma
title	North American Triple Reassortant and Eurasian H1N1 Swine Influenza Viruses Do Not Readily Reassort to Generate a 2009 Pandemic H1N1-Like Virus
title_short	North American Triple Reassortant and Eurasian H1N1 Swine Influenza Viruses Do Not Readily Reassort to Generate a 2009 Pandemic H1N1-Like Virus
title_full	North American Triple Reassortant and Eurasian H1N1 Swine Influenza Viruses Do Not Readily Reassort to Generate a 2009 Pandemic H1N1-Like Virus
title_fullStr	North American Triple Reassortant and Eurasian H1N1 Swine Influenza Viruses Do Not Readily Reassort to Generate a 2009 Pandemic H1N1-Like Virus
title_full_unstemmed	North American Triple Reassortant and Eurasian H1N1 Swine Influenza Viruses Do Not Readily Reassort to Generate a 2009 Pandemic H1N1-Like Virus
title_sort	north american triple reassortant and eurasian h1n1 swine influenza viruses do not readily reassort to generate a 2009 pandemic h1n1-like virus
publisher	American Society for Microbiology
publishDate	2014
url	https://doaj.org/article/b7b5d9163fbf4a7aa9357db409fad9c3
work_keys_str_mv	AT wenjunma northamericantriplereassortantandeurasianh1n1swineinfluenzavirusesdonotreadilyreassorttogeneratea2009pandemich1n1likevirus AT qinfangliu northamericantriplereassortantandeurasianh1n1swineinfluenzavirusesdonotreadilyreassorttogeneratea2009pandemich1n1likevirus AT chuanlingqiao northamericantriplereassortantandeurasianh1n1swineinfluenzavirusesdonotreadilyreassorttogeneratea2009pandemich1n1likevirus AT gustavodelreal northamericantriplereassortantandeurasianh1n1swineinfluenzavirusesdonotreadilyreassorttogeneratea2009pandemich1n1likevirus AT adolfogarciasastre northamericantriplereassortantandeurasianh1n1swineinfluenzavirusesdonotreadilyreassorttogeneratea2009pandemich1n1likevirus AT richardjwebby northamericantriplereassortantandeurasianh1n1swineinfluenzavirusesdonotreadilyreassorttogeneratea2009pandemich1n1likevirus AT jurgenaricht northamericantriplereassortantandeurasianh1n1swineinfluenzavirusesdonotreadilyreassorttogeneratea2009pandemich1n1likevirus
_version_	1718427607500849152

North American Triple Reassortant and Eurasian H1N1 Swine Influenza Viruses Do Not Readily Reassort to Generate a 2009 Pandemic H1N1-Like Virus

Ejemplares similares