Enzymatic Functionalization of Wood as an Antifouling Strategy against the Marine Bacterium <i>Cobetia marina</i>

The protection of wood in marine environments is a major challenge due to the high sensitivity of wood to both water and marine microorganisms. Besides, the environmental regulations are pushing the industry to develop novel effective and environmentally friendly treatments to protect wood in marine...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Daniel Filgueira, Cristian Bolaño, Susana Gouveia, Diego Moldes
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	laccase lauryl gallate wood <i>Cobetia marina</i> antifouling Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/b7b656b76fc746ffa7b547b128970d92
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b7b656b76fc746ffa7b547b128970d92
record_format	dspace
spelling	oai:doaj.org-article:b7b656b76fc746ffa7b547b128970d922021-11-11T18:47:37ZEnzymatic Functionalization of Wood as an Antifouling Strategy against the Marine Bacterium <i>Cobetia marina</i>10.3390/polym132137952073-4360https://doaj.org/article/b7b656b76fc746ffa7b547b128970d922021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4360/13/21/3795https://doaj.org/toc/2073-4360The protection of wood in marine environments is a major challenge due to the high sensitivity of wood to both water and marine microorganisms. Besides, the environmental regulations are pushing the industry to develop novel effective and environmentally friendly treatments to protect wood in marine environments. The present study focused on the development of a new green methodology based on the laccase-assisted grafting of lauryl gallate (LG) onto wood to improve its marine antifouling properties. Initially, the enzymatic treatment conditions (laccase dose, time of reaction, LG concentration) and the effect of the wood specie (beech, pine, and eucalyptus) were assessed by water contact angle (WCA) measurements. The surface properties of the enzymatically modified wood veneers were assessed by X-ray photoelectron spectroscopy (XPS), Fourier transform-infrared spectroscopy (FTIR). Antifouling properties of the functionalized wood veneers against marine bacterium <i>Cobetia marina</i> were studied by scanning electron microscopy (SEM) and protein measurements. XPS and FTIR analysis suggested the stable grafting of LG onto the surface of wood veneers after laccase-assisted treatment. WCA measurements showed that the hydrophobicity of the wood veneers significantly increased after the enzymatic treatment. Protein measurements and SEM pictures showed that enzymatically-hydrophobized wood veneers modified the pattern of bacterial attachment and remarkably reduced the bacterium colonization. Thus, the results observed in the present study confirmed the potential efficiency of laccase-assisted treatments to improve the marine antifouling properties of wood.Daniel FilgueiraCristian BolañoSusana GouveiaDiego MoldesMDPI AGarticlelaccaselauryl gallatewood<i>Cobetia marina</i>antifoulingOrganic chemistryQD241-441ENPolymers, Vol 13, Iss 3795, p 3795 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	laccase lauryl gallate wood <i>Cobetia marina</i> antifouling Organic chemistry QD241-441
spellingShingle	laccase lauryl gallate wood <i>Cobetia marina</i> antifouling Organic chemistry QD241-441 Daniel Filgueira Cristian Bolaño Susana Gouveia Diego Moldes Enzymatic Functionalization of Wood as an Antifouling Strategy against the Marine Bacterium <i>Cobetia marina</i>
description	The protection of wood in marine environments is a major challenge due to the high sensitivity of wood to both water and marine microorganisms. Besides, the environmental regulations are pushing the industry to develop novel effective and environmentally friendly treatments to protect wood in marine environments. The present study focused on the development of a new green methodology based on the laccase-assisted grafting of lauryl gallate (LG) onto wood to improve its marine antifouling properties. Initially, the enzymatic treatment conditions (laccase dose, time of reaction, LG concentration) and the effect of the wood specie (beech, pine, and eucalyptus) were assessed by water contact angle (WCA) measurements. The surface properties of the enzymatically modified wood veneers were assessed by X-ray photoelectron spectroscopy (XPS), Fourier transform-infrared spectroscopy (FTIR). Antifouling properties of the functionalized wood veneers against marine bacterium <i>Cobetia marina</i> were studied by scanning electron microscopy (SEM) and protein measurements. XPS and FTIR analysis suggested the stable grafting of LG onto the surface of wood veneers after laccase-assisted treatment. WCA measurements showed that the hydrophobicity of the wood veneers significantly increased after the enzymatic treatment. Protein measurements and SEM pictures showed that enzymatically-hydrophobized wood veneers modified the pattern of bacterial attachment and remarkably reduced the bacterium colonization. Thus, the results observed in the present study confirmed the potential efficiency of laccase-assisted treatments to improve the marine antifouling properties of wood.
format	article
author	Daniel Filgueira Cristian Bolaño Susana Gouveia Diego Moldes
author_facet	Daniel Filgueira Cristian Bolaño Susana Gouveia Diego Moldes
author_sort	Daniel Filgueira
title	Enzymatic Functionalization of Wood as an Antifouling Strategy against the Marine Bacterium <i>Cobetia marina</i>
title_short	Enzymatic Functionalization of Wood as an Antifouling Strategy against the Marine Bacterium <i>Cobetia marina</i>
title_full	Enzymatic Functionalization of Wood as an Antifouling Strategy against the Marine Bacterium <i>Cobetia marina</i>
title_fullStr	Enzymatic Functionalization of Wood as an Antifouling Strategy against the Marine Bacterium <i>Cobetia marina</i>
title_full_unstemmed	Enzymatic Functionalization of Wood as an Antifouling Strategy against the Marine Bacterium <i>Cobetia marina</i>
title_sort	enzymatic functionalization of wood as an antifouling strategy against the marine bacterium <i>cobetia marina</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b7b656b76fc746ffa7b547b128970d92
work_keys_str_mv	AT danielfilgueira enzymaticfunctionalizationofwoodasanantifoulingstrategyagainstthemarinebacteriumicobetiamarinai AT cristianbolano enzymaticfunctionalizationofwoodasanantifoulingstrategyagainstthemarinebacteriumicobetiamarinai AT susanagouveia enzymaticfunctionalizationofwoodasanantifoulingstrategyagainstthemarinebacteriumicobetiamarinai AT diegomoldes enzymaticfunctionalizationofwoodasanantifoulingstrategyagainstthemarinebacteriumicobetiamarinai
_version_	1718431722398285824

Enzymatic Functionalization of Wood as an Antifouling Strategy against the Marine Bacterium <i>Cobetia marina</i>

Ejemplares similares