Characteristics and Pathogenicity of the Cell-Adapted Attenuated Porcine Epidemic Diarrhea Virus of the Non-S INDEL Cluster

The high antigenic diversity of porcine epidemic diarrhea virus (PEDV) means that porcine epidemic diarrhea (PED) is a challenge for the global pig industry. Understanding the circulation of the virus to determine an optimal vaccine strategy is important in controlling the disease. In this study, we...

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Autores principales:	Thi Thu Hang Vu, Minjoo Yeom, Hyoungjoon Moon, Thi Nhan Tran, Van Phan Le, Daesub Song
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	porcine epidemic diarrhea attenuated vaccine pathogenicity LLC-MK2 genetic variations Medicine R
Acceso en línea:	https://doaj.org/article/b7b73e1e8048499cb1488aa247f4a754
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spelling	oai:doaj.org-article:b7b73e1e8048499cb1488aa247f4a7542021-11-25T18:38:34ZCharacteristics and Pathogenicity of the Cell-Adapted Attenuated Porcine Epidemic Diarrhea Virus of the Non-S INDEL Cluster10.3390/pathogens101114792076-0817https://doaj.org/article/b7b73e1e8048499cb1488aa247f4a7542021-11-01T00:00:00Zhttps://www.mdpi.com/2076-0817/10/11/1479https://doaj.org/toc/2076-0817The high antigenic diversity of porcine epidemic diarrhea virus (PEDV) means that porcine epidemic diarrhea (PED) is a challenge for the global pig industry. Understanding the circulation of the virus to determine an optimal vaccine strategy is important in controlling the disease. In this study, we describe the genetic diversity of circulating PEDV based on the full sequences of spike genes of eight positive samples collected in Vietnam since 2018. Additionally, we developed a live attenuated vaccine candidate from the cell-adapted PEDV2 strain, which was continuously passaged until level 103 in VERO-CCL81 cells. PEDV2-p103, which belongs to the emerging non-S INDEL cluster, exhibited low virus shedding, did not induce lesions in the small intestine of challenged piglets, and had a high titer in the VERO-CCL81 cell at 48 h post-infection. These results suggest that the PEDV2-p103 strain could be a potential oral attenuated vaccine, and its immunogenicity and efficacy should be further assessed through in vivo tests.Thi Thu Hang VuMinjoo YeomHyoungjoon MoonThi Nhan TranVan Phan LeDaesub SongMDPI AGarticleporcine epidemic diarrheaattenuated vaccinepathogenicityLLC-MK2genetic variationsMedicineRENPathogens, Vol 10, Iss 1479, p 1479 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	porcine epidemic diarrhea attenuated vaccine pathogenicity LLC-MK2 genetic variations Medicine R
spellingShingle	porcine epidemic diarrhea attenuated vaccine pathogenicity LLC-MK2 genetic variations Medicine R Thi Thu Hang Vu Minjoo Yeom Hyoungjoon Moon Thi Nhan Tran Van Phan Le Daesub Song Characteristics and Pathogenicity of the Cell-Adapted Attenuated Porcine Epidemic Diarrhea Virus of the Non-S INDEL Cluster
description	The high antigenic diversity of porcine epidemic diarrhea virus (PEDV) means that porcine epidemic diarrhea (PED) is a challenge for the global pig industry. Understanding the circulation of the virus to determine an optimal vaccine strategy is important in controlling the disease. In this study, we describe the genetic diversity of circulating PEDV based on the full sequences of spike genes of eight positive samples collected in Vietnam since 2018. Additionally, we developed a live attenuated vaccine candidate from the cell-adapted PEDV2 strain, which was continuously passaged until level 103 in VERO-CCL81 cells. PEDV2-p103, which belongs to the emerging non-S INDEL cluster, exhibited low virus shedding, did not induce lesions in the small intestine of challenged piglets, and had a high titer in the VERO-CCL81 cell at 48 h post-infection. These results suggest that the PEDV2-p103 strain could be a potential oral attenuated vaccine, and its immunogenicity and efficacy should be further assessed through in vivo tests.
format	article
author	Thi Thu Hang Vu Minjoo Yeom Hyoungjoon Moon Thi Nhan Tran Van Phan Le Daesub Song
author_facet	Thi Thu Hang Vu Minjoo Yeom Hyoungjoon Moon Thi Nhan Tran Van Phan Le Daesub Song
author_sort	Thi Thu Hang Vu
title	Characteristics and Pathogenicity of the Cell-Adapted Attenuated Porcine Epidemic Diarrhea Virus of the Non-S INDEL Cluster
title_short	Characteristics and Pathogenicity of the Cell-Adapted Attenuated Porcine Epidemic Diarrhea Virus of the Non-S INDEL Cluster
title_full	Characteristics and Pathogenicity of the Cell-Adapted Attenuated Porcine Epidemic Diarrhea Virus of the Non-S INDEL Cluster
title_fullStr	Characteristics and Pathogenicity of the Cell-Adapted Attenuated Porcine Epidemic Diarrhea Virus of the Non-S INDEL Cluster
title_full_unstemmed	Characteristics and Pathogenicity of the Cell-Adapted Attenuated Porcine Epidemic Diarrhea Virus of the Non-S INDEL Cluster
title_sort	characteristics and pathogenicity of the cell-adapted attenuated porcine epidemic diarrhea virus of the non-s indel cluster
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b7b73e1e8048499cb1488aa247f4a754
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Characteristics and Pathogenicity of the Cell-Adapted Attenuated Porcine Epidemic Diarrhea Virus of the Non-S INDEL Cluster

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