Internal guide RNA interactions interfere with Cas9-mediated cleavage

Internal guide RNA interactions interfere with Cas9-mediated cleavage

While the CRISPR-Cas9 system has revolutionised molecular biology, it is still a mystery why not every guide RNA elicits target DNA cleavage. Here the authors show that genomic context and internal gRNA interactions can inhibit cleavage.

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Autores principales: Summer B. Thyme, Laila Akhmetova, Tessa G. Montague, Eivind Valen, Alexander F. Schier
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2016
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Science
Q
Acceso en línea:https://doaj.org/article/b7ba7609830548a29b3cdd09a5836f93
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spelling oai:doaj.org-article:b7ba7609830548a29b3cdd09a5836f932021-12-02T15:34:08ZInternal guide RNA interactions interfere with Cas9-mediated cleavage10.1038/ncomms117502041-1723https://doaj.org/article/b7ba7609830548a29b3cdd09a5836f932016-06-01T00:00:00Zhttps://doi.org/10.1038/ncomms11750https://doaj.org/toc/2041-1723While the CRISPR-Cas9 system has revolutionised molecular biology, it is still a mystery why not every guide RNA elicits target DNA cleavage. Here the authors show that genomic context and internal gRNA interactions can inhibit cleavage.Summer B. ThymeLaila AkhmetovaTessa G. MontagueEivind ValenAlexander F. SchierNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-7 (2016)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Summer B. Thyme
Laila Akhmetova
Tessa G. Montague
Eivind Valen
Alexander F. Schier
Internal guide RNA interactions interfere with Cas9-mediated cleavage
description While the CRISPR-Cas9 system has revolutionised molecular biology, it is still a mystery why not every guide RNA elicits target DNA cleavage. Here the authors show that genomic context and internal gRNA interactions can inhibit cleavage.
format article
author Summer B. Thyme
Laila Akhmetova
Tessa G. Montague
Eivind Valen
Alexander F. Schier
author_facet Summer B. Thyme
Laila Akhmetova
Tessa G. Montague
Eivind Valen
Alexander F. Schier
author_sort Summer B. Thyme
title Internal guide RNA interactions interfere with Cas9-mediated cleavage
title_short Internal guide RNA interactions interfere with Cas9-mediated cleavage
title_full Internal guide RNA interactions interfere with Cas9-mediated cleavage
title_fullStr Internal guide RNA interactions interfere with Cas9-mediated cleavage
title_full_unstemmed Internal guide RNA interactions interfere with Cas9-mediated cleavage
title_sort internal guide rna interactions interfere with cas9-mediated cleavage
publisher Nature Portfolio
publishDate 2016
url https://doaj.org/article/b7ba7609830548a29b3cdd09a5836f93
work_keys_str_mv AT summerbthyme internalguidernainteractionsinterferewithcas9mediatedcleavage
AT lailaakhmetova internalguidernainteractionsinterferewithcas9mediatedcleavage
AT tessagmontague internalguidernainteractionsinterferewithcas9mediatedcleavage
AT eivindvalen internalguidernainteractionsinterferewithcas9mediatedcleavage
AT alexanderfschier internalguidernainteractionsinterferewithcas9mediatedcleavage
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