Current Analytical Strategies in Studying Chromatin-Associated-Proteome (Chromatome)

Chromatin is a dynamic structure comprising of DNA and proteins. Its unique nature not only help to pack the DNA tightly within the cell but also is pivotal in regulating gene expression DNA replication. Furthermore it also protects the DNA from being damaged. Various proteins are involved in making...

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Autores principales:	Niamat Khan, Sidra Shahid, Abdul R. Asif
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	chromatin euchromatin heterochromatin interphase centromere telomere Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/b7c33cef56524a038a479a14d709b6fd
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id	oai:doaj.org-article:b7c33cef56524a038a479a14d709b6fd
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spelling	oai:doaj.org-article:b7c33cef56524a038a479a14d709b6fd2021-11-11T18:38:44ZCurrent Analytical Strategies in Studying Chromatin-Associated-Proteome (Chromatome)10.3390/molecules262166941420-3049https://doaj.org/article/b7c33cef56524a038a479a14d709b6fd2021-11-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6694https://doaj.org/toc/1420-3049Chromatin is a dynamic structure comprising of DNA and proteins. Its unique nature not only help to pack the DNA tightly within the cell but also is pivotal in regulating gene expression DNA replication. Furthermore it also protects the DNA from being damaged. Various proteins are involved in making a specific complex within a chromatin and the knowledge about these interacting partners is helpful to enhance our understanding about the pathophysiology of various chromatin associated diseases. Moreover, it could also help us to identify new drug targets and design more effective remedies. Due to the existence of chromatin in different forms under various physiological conditions it is hard to develop a single strategy to study chromatin associated proteins under all conditions. In our current review, we tried to provide an overview and comparative analysis of the strategies currently adopted to capture the DNA bounded protein complexes and their mass spectrometric identification and quantification. Precise information about the protein partners and their function in the DNA-protein complexes is crucial to design new and more effective therapeutic molecules against chromatin associated diseases.Niamat KhanSidra ShahidAbdul R. AsifMDPI AGarticlechromatineuchromatinheterochromatininterphasecentromeretelomereOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6694, p 6694 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chromatin euchromatin heterochromatin interphase centromere telomere Organic chemistry QD241-441
spellingShingle	chromatin euchromatin heterochromatin interphase centromere telomere Organic chemistry QD241-441 Niamat Khan Sidra Shahid Abdul R. Asif Current Analytical Strategies in Studying Chromatin-Associated-Proteome (Chromatome)
description	Chromatin is a dynamic structure comprising of DNA and proteins. Its unique nature not only help to pack the DNA tightly within the cell but also is pivotal in regulating gene expression DNA replication. Furthermore it also protects the DNA from being damaged. Various proteins are involved in making a specific complex within a chromatin and the knowledge about these interacting partners is helpful to enhance our understanding about the pathophysiology of various chromatin associated diseases. Moreover, it could also help us to identify new drug targets and design more effective remedies. Due to the existence of chromatin in different forms under various physiological conditions it is hard to develop a single strategy to study chromatin associated proteins under all conditions. In our current review, we tried to provide an overview and comparative analysis of the strategies currently adopted to capture the DNA bounded protein complexes and their mass spectrometric identification and quantification. Precise information about the protein partners and their function in the DNA-protein complexes is crucial to design new and more effective therapeutic molecules against chromatin associated diseases.
format	article
author	Niamat Khan Sidra Shahid Abdul R. Asif
author_facet	Niamat Khan Sidra Shahid Abdul R. Asif
author_sort	Niamat Khan
title	Current Analytical Strategies in Studying Chromatin-Associated-Proteome (Chromatome)
title_short	Current Analytical Strategies in Studying Chromatin-Associated-Proteome (Chromatome)
title_full	Current Analytical Strategies in Studying Chromatin-Associated-Proteome (Chromatome)
title_fullStr	Current Analytical Strategies in Studying Chromatin-Associated-Proteome (Chromatome)
title_full_unstemmed	Current Analytical Strategies in Studying Chromatin-Associated-Proteome (Chromatome)
title_sort	current analytical strategies in studying chromatin-associated-proteome (chromatome)
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b7c33cef56524a038a479a14d709b6fd
work_keys_str_mv	AT niamatkhan currentanalyticalstrategiesinstudyingchromatinassociatedproteomechromatome AT sidrashahid currentanalyticalstrategiesinstudyingchromatinassociatedproteomechromatome AT abdulrasif currentanalyticalstrategiesinstudyingchromatinassociatedproteomechromatome
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Current Analytical Strategies in Studying Chromatin-Associated-Proteome (Chromatome)

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