Effect of nanofiber content on bone regeneration of silk fibroin/poly(ε-caprolactone) nano/microfibrous composite scaffolds

Effect of nanofiber content on bone regeneration of silk fibroin/poly(ε-caprolactone) nano/microfibrous composite scaffolds

Beom Su Kim,1,2,* Ko Eun Park,3,4,* Min Hee Kim,3 Hyung Keun You,5 Jun Lee,1 Won Ho Park3 1Wonkwang Bone Regeneration Institute, Wonkwang University, Iksan, South Korea; 2Bone Cell Biotech, Daejeon, South Korea; 3Department of Advanced Organic Materials and Textile System Engineering, Chungnam Nat...

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Autores principales: Kim BS, Park KE, Kim MH, You HK, Lee J, Park WH
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2015
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Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/b7caf652ebc0421e8a0579b0e8ee366d
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spelling oai:doaj.org-article:b7caf652ebc0421e8a0579b0e8ee366d2021-12-02T02:21:11ZEffect of nanofiber content on bone regeneration of silk fibroin/poly(ε-caprolactone) nano/microfibrous composite scaffolds1178-2013https://doaj.org/article/b7caf652ebc0421e8a0579b0e8ee366d2015-01-01T00:00:00Zhttp://www.dovepress.com/effect-of-nanofiber-content-on-bone-regeneration-of-silk-fibroinpolyep-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Beom Su Kim,1,2,* Ko Eun Park,3,4,* Min Hee Kim,3 Hyung Keun You,5 Jun Lee,1 Won Ho Park3 1Wonkwang Bone Regeneration Institute, Wonkwang University, Iksan, South Korea; 2Bone Cell Biotech, Daejeon, South Korea; 3Department of Advanced Organic Materials and Textile System Engineering, Chungnam National University, Daejeon, South Korea; 4Central Research Institute, Humedix, Anyang, South Korea; 5Department of Periodontology, School of Dentistry, Wonkwang University, Iksan, South Korea *These two authors contributed equally to this work Abstract: The broad application of electrospun nanofibrous scaffolds in tissue engineering is limited by their small pore size, which has a negative influence on cell migration. This disadvantage could be significantly improved through the combination of nano- and microfibrous structure. To accomplish this, different nano/microfibrous scaffolds were produced by hybrid electrospinning, combining solution electrospinning with melt electrospinning, while varying the content of the nanofiber. The morphology of the silk fibroin (SF)/poly(ε-caprolactone) (PCL) nano/microfibrous composite scaffolds was investigated with field-emission scanning electron microscopy, while the mechanical and pore properties were assessed by measurement of tensile strength and mercury porosimetry. To assay cell proliferation, cell viability, and infiltration ability, human mesenchymal stem cells were seeded on the SF/PCL nano/microfibrous composite scaffolds. From in vivo tests, it was found that the bone-regenerating ability of SF/PCL nano/microfibrous composite scaffolds was closely associated with the nanofiber content in the composite scaffolds. In conclusion, this approach of controlling the nanofiber content in SF/PCL nano/microfibrous composite scaffolds could be useful in the design of novel scaffolds for tissue engineering. Keywords: silk fibroin (SF), poly(ε-caprolactone) (PCL), nanofibers, microfibers, composite scaffolds, bone regenerationKim BSPark KEKim MHYou HKLee JPark WHDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 485-502 (2015)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Kim BS
Park KE
Kim MH
You HK
Lee J
Park WH
Effect of nanofiber content on bone regeneration of silk fibroin/poly(ε-caprolactone) nano/microfibrous composite scaffolds
description Beom Su Kim,1,2,* Ko Eun Park,3,4,* Min Hee Kim,3 Hyung Keun You,5 Jun Lee,1 Won Ho Park3 1Wonkwang Bone Regeneration Institute, Wonkwang University, Iksan, South Korea; 2Bone Cell Biotech, Daejeon, South Korea; 3Department of Advanced Organic Materials and Textile System Engineering, Chungnam National University, Daejeon, South Korea; 4Central Research Institute, Humedix, Anyang, South Korea; 5Department of Periodontology, School of Dentistry, Wonkwang University, Iksan, South Korea *These two authors contributed equally to this work Abstract: The broad application of electrospun nanofibrous scaffolds in tissue engineering is limited by their small pore size, which has a negative influence on cell migration. This disadvantage could be significantly improved through the combination of nano- and microfibrous structure. To accomplish this, different nano/microfibrous scaffolds were produced by hybrid electrospinning, combining solution electrospinning with melt electrospinning, while varying the content of the nanofiber. The morphology of the silk fibroin (SF)/poly(ε-caprolactone) (PCL) nano/microfibrous composite scaffolds was investigated with field-emission scanning electron microscopy, while the mechanical and pore properties were assessed by measurement of tensile strength and mercury porosimetry. To assay cell proliferation, cell viability, and infiltration ability, human mesenchymal stem cells were seeded on the SF/PCL nano/microfibrous composite scaffolds. From in vivo tests, it was found that the bone-regenerating ability of SF/PCL nano/microfibrous composite scaffolds was closely associated with the nanofiber content in the composite scaffolds. In conclusion, this approach of controlling the nanofiber content in SF/PCL nano/microfibrous composite scaffolds could be useful in the design of novel scaffolds for tissue engineering. Keywords: silk fibroin (SF), poly(ε-caprolactone) (PCL), nanofibers, microfibers, composite scaffolds, bone regeneration
format article
author Kim BS
Park KE
Kim MH
You HK
Lee J
Park WH
author_facet Kim BS
Park KE
Kim MH
You HK
Lee J
Park WH
author_sort Kim BS
title Effect of nanofiber content on bone regeneration of silk fibroin/poly(ε-caprolactone) nano/microfibrous composite scaffolds
title_short Effect of nanofiber content on bone regeneration of silk fibroin/poly(ε-caprolactone) nano/microfibrous composite scaffolds
title_full Effect of nanofiber content on bone regeneration of silk fibroin/poly(ε-caprolactone) nano/microfibrous composite scaffolds
title_fullStr Effect of nanofiber content on bone regeneration of silk fibroin/poly(ε-caprolactone) nano/microfibrous composite scaffolds
title_full_unstemmed Effect of nanofiber content on bone regeneration of silk fibroin/poly(ε-caprolactone) nano/microfibrous composite scaffolds
title_sort effect of nanofiber content on bone regeneration of silk fibroin/poly(ε-caprolactone) nano/microfibrous composite scaffolds
publisher Dove Medical Press
publishDate 2015
url https://doaj.org/article/b7caf652ebc0421e8a0579b0e8ee366d
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