Evaluation of quasi-static and dynamic nanomechanical properties of bone-metastatic breast cancer cells using a nanoclay cancer testbed

Abstract In recent years, there has been increasing interest in investigating the mechanical properties of individual cells to delineate disease mechanisms. Reorganization of cytoskeleton facilitates the colonization of metastatic breast cancer at bone marrow space, leading to bone metastasis. Here,...

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Autores principales:	Sumanta Kar, Dinesh R. Katti, Kalpana S. Katti
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b7cc82fd763e40749a32f36f8e01b7ec
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spelling	oai:doaj.org-article:b7cc82fd763e40749a32f36f8e01b7ec2021-12-02T14:06:56ZEvaluation of quasi-static and dynamic nanomechanical properties of bone-metastatic breast cancer cells using a nanoclay cancer testbed10.1038/s41598-021-82664-92045-2322https://doaj.org/article/b7cc82fd763e40749a32f36f8e01b7ec2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82664-9https://doaj.org/toc/2045-2322Abstract In recent years, there has been increasing interest in investigating the mechanical properties of individual cells to delineate disease mechanisms. Reorganization of cytoskeleton facilitates the colonization of metastatic breast cancer at bone marrow space, leading to bone metastasis. Here, we report evaluation of mechanical properties of two breast cancer cells with different metastatic ability at the site of bone metastases, using quasi-static and dynamic nanoindentation methods. Our results showed that the significant reduction in elastic modulus along with increased liquid-like behavior of bone metastasized MCF-7 cells was induced by depolymerization and reorganization of F-actin to the adherens junctions, whereas bone metastasized MDA-MB-231 cells showed insignificant changes in elastic modulus and F-actin reorganization over time, compared to their respective as-received counterparts. Taken together, our data demonstrate evolution of breast cancer cell mechanics at bone metastases.Sumanta KarDinesh R. KattiKalpana S. KattiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sumanta Kar Dinesh R. Katti Kalpana S. Katti Evaluation of quasi-static and dynamic nanomechanical properties of bone-metastatic breast cancer cells using a nanoclay cancer testbed
description	Abstract In recent years, there has been increasing interest in investigating the mechanical properties of individual cells to delineate disease mechanisms. Reorganization of cytoskeleton facilitates the colonization of metastatic breast cancer at bone marrow space, leading to bone metastasis. Here, we report evaluation of mechanical properties of two breast cancer cells with different metastatic ability at the site of bone metastases, using quasi-static and dynamic nanoindentation methods. Our results showed that the significant reduction in elastic modulus along with increased liquid-like behavior of bone metastasized MCF-7 cells was induced by depolymerization and reorganization of F-actin to the adherens junctions, whereas bone metastasized MDA-MB-231 cells showed insignificant changes in elastic modulus and F-actin reorganization over time, compared to their respective as-received counterparts. Taken together, our data demonstrate evolution of breast cancer cell mechanics at bone metastases.
format	article
author	Sumanta Kar Dinesh R. Katti Kalpana S. Katti
author_facet	Sumanta Kar Dinesh R. Katti Kalpana S. Katti
author_sort	Sumanta Kar
title	Evaluation of quasi-static and dynamic nanomechanical properties of bone-metastatic breast cancer cells using a nanoclay cancer testbed
title_short	Evaluation of quasi-static and dynamic nanomechanical properties of bone-metastatic breast cancer cells using a nanoclay cancer testbed
title_full	Evaluation of quasi-static and dynamic nanomechanical properties of bone-metastatic breast cancer cells using a nanoclay cancer testbed
title_fullStr	Evaluation of quasi-static and dynamic nanomechanical properties of bone-metastatic breast cancer cells using a nanoclay cancer testbed
title_full_unstemmed	Evaluation of quasi-static and dynamic nanomechanical properties of bone-metastatic breast cancer cells using a nanoclay cancer testbed
title_sort	evaluation of quasi-static and dynamic nanomechanical properties of bone-metastatic breast cancer cells using a nanoclay cancer testbed
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b7cc82fd763e40749a32f36f8e01b7ec
work_keys_str_mv	AT sumantakar evaluationofquasistaticanddynamicnanomechanicalpropertiesofbonemetastaticbreastcancercellsusingananoclaycancertestbed AT dineshrkatti evaluationofquasistaticanddynamicnanomechanicalpropertiesofbonemetastaticbreastcancercellsusingananoclaycancertestbed AT kalpanaskatti evaluationofquasistaticanddynamicnanomechanicalpropertiesofbonemetastaticbreastcancercellsusingananoclaycancertestbed
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Evaluation of quasi-static and dynamic nanomechanical properties of bone-metastatic breast cancer cells using a nanoclay cancer testbed

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