Characterization, Antioxidant and Antitumor Activities of Oligosaccharides Isolated from Evodia lepta (Spreng) Merr. by Different Extraction Methods

Evodia lepta (E. lepta) is a traditional Chinese herbal medicine with various biological activities. One of the active components of this widely used medicinal plant is believed to be an oligosaccharide. The extraction yields, structural characteristics, antioxi...

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Autores principales:	Feng Xiong, Hui-Xian Liang, Zhi-Jing Zhang, Taifo Mahmud, Albert S. C. Chan, Xia Li, Wen-Jian Lan
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Evodia lepta</i> oligosaccharide antioxidant antitumor Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/b7dcdde11ad04220999a99eb774acf0d
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spelling	oai:doaj.org-article:b7dcdde11ad04220999a99eb774acf0d2021-11-25T16:29:43ZCharacterization, Antioxidant and Antitumor Activities of Oligosaccharides Isolated from <i>Evodia lepta</i> (Spreng) Merr. by Different Extraction Methods10.3390/antiox101118422076-3921https://doaj.org/article/b7dcdde11ad04220999a99eb774acf0d2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-3921/10/11/1842https://doaj.org/toc/2076-3921<i>Evodia lepta</i> (<i>E. lepta</i>) is a traditional Chinese herbal medicine with various biological activities. One of the active components of this widely used medicinal plant is believed to be an oligosaccharide. The extraction yields, structural characteristics, antioxidant, and antitumor activities of four oligosaccharide extracts obtained by hot water extraction (HEO), ultrasound-assisted extraction (UEO), enzyme-assisted (EEO), and microwave-assisted extraction (MEO) were investigated. Matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS), X-ray diffraction (XRD), and Scanning electron microscopy (SEM) results indicated that the extraction methods had a difference on the molecular mass distribution, structure, and morphology of the EOs. In addition, HEO and MEO showed strong antioxidant activities, which might be related to their uronic acid and protein contents. More interestingly, MEO was more active toward MDA-MB-231 cells compared to other cells, and cell growth inhibition was proposed to occur through apoptosis. Overall, microwave-assisted extraction is a promising technique for the extraction of high quality EO.Feng XiongHui-Xian LiangZhi-Jing ZhangTaifo MahmudAlbert S. C. ChanXia LiWen-Jian LanMDPI AGarticle<i>Evodia lepta</i>oligosaccharideantioxidantantitumorTherapeutics. PharmacologyRM1-950ENAntioxidants, Vol 10, Iss 1842, p 1842 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Evodia lepta</i> oligosaccharide antioxidant antitumor Therapeutics. Pharmacology RM1-950
spellingShingle	<i>Evodia lepta</i> oligosaccharide antioxidant antitumor Therapeutics. Pharmacology RM1-950 Feng Xiong Hui-Xian Liang Zhi-Jing Zhang Taifo Mahmud Albert S. C. Chan Xia Li Wen-Jian Lan Characterization, Antioxidant and Antitumor Activities of Oligosaccharides Isolated from <i>Evodia lepta</i> (Spreng) Merr. by Different Extraction Methods
description	<i>Evodia lepta</i> (<i>E. lepta</i>) is a traditional Chinese herbal medicine with various biological activities. One of the active components of this widely used medicinal plant is believed to be an oligosaccharide. The extraction yields, structural characteristics, antioxidant, and antitumor activities of four oligosaccharide extracts obtained by hot water extraction (HEO), ultrasound-assisted extraction (UEO), enzyme-assisted (EEO), and microwave-assisted extraction (MEO) were investigated. Matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS), X-ray diffraction (XRD), and Scanning electron microscopy (SEM) results indicated that the extraction methods had a difference on the molecular mass distribution, structure, and morphology of the EOs. In addition, HEO and MEO showed strong antioxidant activities, which might be related to their uronic acid and protein contents. More interestingly, MEO was more active toward MDA-MB-231 cells compared to other cells, and cell growth inhibition was proposed to occur through apoptosis. Overall, microwave-assisted extraction is a promising technique for the extraction of high quality EO.
format	article
author	Feng Xiong Hui-Xian Liang Zhi-Jing Zhang Taifo Mahmud Albert S. C. Chan Xia Li Wen-Jian Lan
author_facet	Feng Xiong Hui-Xian Liang Zhi-Jing Zhang Taifo Mahmud Albert S. C. Chan Xia Li Wen-Jian Lan
author_sort	Feng Xiong
title	Characterization, Antioxidant and Antitumor Activities of Oligosaccharides Isolated from <i>Evodia lepta</i> (Spreng) Merr. by Different Extraction Methods
title_short	Characterization, Antioxidant and Antitumor Activities of Oligosaccharides Isolated from <i>Evodia lepta</i> (Spreng) Merr. by Different Extraction Methods
title_full	Characterization, Antioxidant and Antitumor Activities of Oligosaccharides Isolated from <i>Evodia lepta</i> (Spreng) Merr. by Different Extraction Methods
title_fullStr	Characterization, Antioxidant and Antitumor Activities of Oligosaccharides Isolated from <i>Evodia lepta</i> (Spreng) Merr. by Different Extraction Methods
title_full_unstemmed	Characterization, Antioxidant and Antitumor Activities of Oligosaccharides Isolated from <i>Evodia lepta</i> (Spreng) Merr. by Different Extraction Methods
title_sort	characterization, antioxidant and antitumor activities of oligosaccharides isolated from <i>evodia lepta</i> (spreng) merr. by different extraction methods
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b7dcdde11ad04220999a99eb774acf0d
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Characterization, Antioxidant and Antitumor Activities of Oligosaccharides Isolated from <i>Evodia lepta</i> (Spreng) Merr. by Different Extraction Methods

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