Monocyte chemoattractant protein-1/CCL2 produced by stromal cells promotes lung metastasis of 4T1 murine breast cancer cells.
MCP-1/CCL2 plays an important role in the initiation and progression of cancer. Since tumor cells produce MCP-1, they are considered to be the main source of this chemokine. Here, we examined whether MCP-1 produced by non-tumor cells affects the growth and lung metastasis of 4T1 breast cancer cells...
Guardado en:
| Autores principales: | , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2013
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/b7e48c9ffc7642debae4cc5b40151ce5 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:b7e48c9ffc7642debae4cc5b40151ce5 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:b7e48c9ffc7642debae4cc5b40151ce52021-11-18T07:53:10ZMonocyte chemoattractant protein-1/CCL2 produced by stromal cells promotes lung metastasis of 4T1 murine breast cancer cells.1932-620310.1371/journal.pone.0058791https://doaj.org/article/b7e48c9ffc7642debae4cc5b40151ce52013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23527025/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203MCP-1/CCL2 plays an important role in the initiation and progression of cancer. Since tumor cells produce MCP-1, they are considered to be the main source of this chemokine. Here, we examined whether MCP-1 produced by non-tumor cells affects the growth and lung metastasis of 4T1 breast cancer cells by transplanting them into the mammary pad of WT or MCP-1(-/-) mice. Primary tumors at the injected site grew similarly in both mice; however, lung metastases were markedly reduced in MCP-1(-/-) mice, with significantly longer mouse survival. High levels of MCP-1 mRNA were detected in tumors growing in WT, but not MCP-1(-/-) mice. Serum MCP-1 levels were increased in tumor-bearing WT, but not MCP-1(-/-) mice. Transplantation of MCP-1(-/-) bone marrow cells into WT mice did not alter the incidence of lung metastasis, whereas transplantation of WT bone marrow cells into MCP-1(-/-) mice increased lung metastasis. The primary tumors of MCP-1(-/-) mice consistently developed necrosis earlier than those of WT mice and showed decreased infiltration by macrophages and reduced angiogenesis. Interestingly, 4T1 cells that metastasized to the lung constitutively expressed elevated levels of MCP-1, and intravenous injection of 4T1 cells producing a high level of MCP-1 resulted in increased tumor foci in the lung of WT and MCP-1(-/-) mice. Thus, stromal cell-derived MCP-1 in the primary tumors promotes lung metastasis of 4T1 cells, but tumor cell-derived MCP-1 can also contribute once tumor cells enter the circulation. A greater understanding of the source and role of this chemokine may lead to novel strategies for cancer treatment.Teizo YoshimuraO M Zack HowardToshihiro ItoMasaki KuwabaraAkihiro MatsukawaKeqiang ChenYing LiuMingyong LiuJoost J OppenheimJi Ming WangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e58791 (2013) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Teizo Yoshimura O M Zack Howard Toshihiro Ito Masaki Kuwabara Akihiro Matsukawa Keqiang Chen Ying Liu Mingyong Liu Joost J Oppenheim Ji Ming Wang Monocyte chemoattractant protein-1/CCL2 produced by stromal cells promotes lung metastasis of 4T1 murine breast cancer cells. |
| description |
MCP-1/CCL2 plays an important role in the initiation and progression of cancer. Since tumor cells produce MCP-1, they are considered to be the main source of this chemokine. Here, we examined whether MCP-1 produced by non-tumor cells affects the growth and lung metastasis of 4T1 breast cancer cells by transplanting them into the mammary pad of WT or MCP-1(-/-) mice. Primary tumors at the injected site grew similarly in both mice; however, lung metastases were markedly reduced in MCP-1(-/-) mice, with significantly longer mouse survival. High levels of MCP-1 mRNA were detected in tumors growing in WT, but not MCP-1(-/-) mice. Serum MCP-1 levels were increased in tumor-bearing WT, but not MCP-1(-/-) mice. Transplantation of MCP-1(-/-) bone marrow cells into WT mice did not alter the incidence of lung metastasis, whereas transplantation of WT bone marrow cells into MCP-1(-/-) mice increased lung metastasis. The primary tumors of MCP-1(-/-) mice consistently developed necrosis earlier than those of WT mice and showed decreased infiltration by macrophages and reduced angiogenesis. Interestingly, 4T1 cells that metastasized to the lung constitutively expressed elevated levels of MCP-1, and intravenous injection of 4T1 cells producing a high level of MCP-1 resulted in increased tumor foci in the lung of WT and MCP-1(-/-) mice. Thus, stromal cell-derived MCP-1 in the primary tumors promotes lung metastasis of 4T1 cells, but tumor cell-derived MCP-1 can also contribute once tumor cells enter the circulation. A greater understanding of the source and role of this chemokine may lead to novel strategies for cancer treatment. |
| format |
article |
| author |
Teizo Yoshimura O M Zack Howard Toshihiro Ito Masaki Kuwabara Akihiro Matsukawa Keqiang Chen Ying Liu Mingyong Liu Joost J Oppenheim Ji Ming Wang |
| author_facet |
Teizo Yoshimura O M Zack Howard Toshihiro Ito Masaki Kuwabara Akihiro Matsukawa Keqiang Chen Ying Liu Mingyong Liu Joost J Oppenheim Ji Ming Wang |
| author_sort |
Teizo Yoshimura |
| title |
Monocyte chemoattractant protein-1/CCL2 produced by stromal cells promotes lung metastasis of 4T1 murine breast cancer cells. |
| title_short |
Monocyte chemoattractant protein-1/CCL2 produced by stromal cells promotes lung metastasis of 4T1 murine breast cancer cells. |
| title_full |
Monocyte chemoattractant protein-1/CCL2 produced by stromal cells promotes lung metastasis of 4T1 murine breast cancer cells. |
| title_fullStr |
Monocyte chemoattractant protein-1/CCL2 produced by stromal cells promotes lung metastasis of 4T1 murine breast cancer cells. |
| title_full_unstemmed |
Monocyte chemoattractant protein-1/CCL2 produced by stromal cells promotes lung metastasis of 4T1 murine breast cancer cells. |
| title_sort |
monocyte chemoattractant protein-1/ccl2 produced by stromal cells promotes lung metastasis of 4t1 murine breast cancer cells. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/b7e48c9ffc7642debae4cc5b40151ce5 |
| work_keys_str_mv |
AT teizoyoshimura monocytechemoattractantprotein1ccl2producedbystromalcellspromoteslungmetastasisof4t1murinebreastcancercells AT omzackhoward monocytechemoattractantprotein1ccl2producedbystromalcellspromoteslungmetastasisof4t1murinebreastcancercells AT toshihiroito monocytechemoattractantprotein1ccl2producedbystromalcellspromoteslungmetastasisof4t1murinebreastcancercells AT masakikuwabara monocytechemoattractantprotein1ccl2producedbystromalcellspromoteslungmetastasisof4t1murinebreastcancercells AT akihiromatsukawa monocytechemoattractantprotein1ccl2producedbystromalcellspromoteslungmetastasisof4t1murinebreastcancercells AT keqiangchen monocytechemoattractantprotein1ccl2producedbystromalcellspromoteslungmetastasisof4t1murinebreastcancercells AT yingliu monocytechemoattractantprotein1ccl2producedbystromalcellspromoteslungmetastasisof4t1murinebreastcancercells AT mingyongliu monocytechemoattractantprotein1ccl2producedbystromalcellspromoteslungmetastasisof4t1murinebreastcancercells AT joostjoppenheim monocytechemoattractantprotein1ccl2producedbystromalcellspromoteslungmetastasisof4t1murinebreastcancercells AT jimingwang monocytechemoattractantprotein1ccl2producedbystromalcellspromoteslungmetastasisof4t1murinebreastcancercells |
| _version_ |
1718422821966708736 |