Prokineticin 1–prokineticin receptor 1 signaling in trophoblast promotes embryo implantation and placenta development

Abstract Successful pregnancy establishment in mammals depends on proper embryo-maternal communication. Prokineticin 1 (PROK1) is a secretory protein that exerts pleiotropic functions in various tissues. Despite the studies that have primarily been performed with human cell lines and mice, the funct...

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Autores principales:	Ewelina Goryszewska-Szczurek, Monika Baryla, Piotr Kaczynski, Agnieszka Waclawik
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b7eb0fb1f9e6414882a20b750c7dde60
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spelling	oai:doaj.org-article:b7eb0fb1f9e6414882a20b750c7dde602021-12-02T18:18:33ZProkineticin 1–prokineticin receptor 1 signaling in trophoblast promotes embryo implantation and placenta development10.1038/s41598-021-93102-12045-2322https://doaj.org/article/b7eb0fb1f9e6414882a20b750c7dde602021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93102-1https://doaj.org/toc/2045-2322Abstract Successful pregnancy establishment in mammals depends on proper embryo-maternal communication. Prokineticin 1 (PROK1) is a secretory protein that exerts pleiotropic functions in various tissues. Despite the studies that have primarily been performed with human cell lines and mice, the function of PROK1 in trophoblasts has still not been fully elucidated. Hence, the aim of this study was to establish the role of PROK1 in trophoblasts during implantation and placentation. Prokineticin 1 mRNA was elevated in porcine trophoblasts during implantation and the early placentation period. Furthermore, we reveal that PROK1–PROKR1 signaling induces the expression of genes involved in the regulation of angiogenesis, immunological response, trophoblast cell adhesion, invasion, and proliferation, as well as stimulating phosphorylation of MAPK and PTK2. Ingenuity Pathway Analysis identified the aforementioned and also other functions associated with PROK1-regulated genes/proteins, such as cell-to-cell contact, epithelial tissue differentiation, Ca2+ release, lipid synthesis, and chemotaxis. We also showed evidence that PROK1 acting via PROKR1 increased trophoblast cell proliferation and adhesion. The PROK1-stimulated cell proliferation was mediated by PI3K/AKT/mTOR, MAPK, and cAMP, whereas adhesion was mediated by MAPK and/or PI3K/AKT signaling pathways. Concluding, our study suggests that PROK1 plays a pleiotropic role in trophoblast function during implantation and early placentation.Ewelina Goryszewska-SzczurekMonika BarylaPiotr KaczynskiAgnieszka WaclawikNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ewelina Goryszewska-Szczurek Monika Baryla Piotr Kaczynski Agnieszka Waclawik Prokineticin 1–prokineticin receptor 1 signaling in trophoblast promotes embryo implantation and placenta development
description	Abstract Successful pregnancy establishment in mammals depends on proper embryo-maternal communication. Prokineticin 1 (PROK1) is a secretory protein that exerts pleiotropic functions in various tissues. Despite the studies that have primarily been performed with human cell lines and mice, the function of PROK1 in trophoblasts has still not been fully elucidated. Hence, the aim of this study was to establish the role of PROK1 in trophoblasts during implantation and placentation. Prokineticin 1 mRNA was elevated in porcine trophoblasts during implantation and the early placentation period. Furthermore, we reveal that PROK1–PROKR1 signaling induces the expression of genes involved in the regulation of angiogenesis, immunological response, trophoblast cell adhesion, invasion, and proliferation, as well as stimulating phosphorylation of MAPK and PTK2. Ingenuity Pathway Analysis identified the aforementioned and also other functions associated with PROK1-regulated genes/proteins, such as cell-to-cell contact, epithelial tissue differentiation, Ca2+ release, lipid synthesis, and chemotaxis. We also showed evidence that PROK1 acting via PROKR1 increased trophoblast cell proliferation and adhesion. The PROK1-stimulated cell proliferation was mediated by PI3K/AKT/mTOR, MAPK, and cAMP, whereas adhesion was mediated by MAPK and/or PI3K/AKT signaling pathways. Concluding, our study suggests that PROK1 plays a pleiotropic role in trophoblast function during implantation and early placentation.
format	article
author	Ewelina Goryszewska-Szczurek Monika Baryla Piotr Kaczynski Agnieszka Waclawik
author_facet	Ewelina Goryszewska-Szczurek Monika Baryla Piotr Kaczynski Agnieszka Waclawik
author_sort	Ewelina Goryszewska-Szczurek
title	Prokineticin 1–prokineticin receptor 1 signaling in trophoblast promotes embryo implantation and placenta development
title_short	Prokineticin 1–prokineticin receptor 1 signaling in trophoblast promotes embryo implantation and placenta development
title_full	Prokineticin 1–prokineticin receptor 1 signaling in trophoblast promotes embryo implantation and placenta development
title_fullStr	Prokineticin 1–prokineticin receptor 1 signaling in trophoblast promotes embryo implantation and placenta development
title_full_unstemmed	Prokineticin 1–prokineticin receptor 1 signaling in trophoblast promotes embryo implantation and placenta development
title_sort	prokineticin 1–prokineticin receptor 1 signaling in trophoblast promotes embryo implantation and placenta development
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b7eb0fb1f9e6414882a20b750c7dde60
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Prokineticin 1–prokineticin receptor 1 signaling in trophoblast promotes embryo implantation and placenta development

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