A generic approach to study the kinetics of liquid–liquid phase separation under near-native conditions

Van Lindt, Bratek-Skicki et al. show that at carefully selected pH values, proteins can be kept in solution and their LLPS can then be induced by a jump to native pH. This presents a generic method to study the full kinetic trajectory of LLPS under near native conditions that can be easily controlle...

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Autores principales: Joris Van Lindt, Anna Bratek-Skicki, Phuong N. Nguyen, Donya Pakravan, Luis F. Durán-Armenta, Agnes Tantos, Rita Pancsa, Ludo Van Den Bosch, Dominique Maes, Peter Tompa
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/b7ebf01cce4d42dd989460bcbd0ea84b
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Sumario:Van Lindt, Bratek-Skicki et al. show that at carefully selected pH values, proteins can be kept in solution and their LLPS can then be induced by a jump to native pH. This presents a generic method to study the full kinetic trajectory of LLPS under near native conditions that can be easily controlled, providing a platform for the characterization of physiologically relevant phase-separation behaviour of diverse proteins.