A generic approach to study the kinetics of liquid–liquid phase separation under near-native conditions

Van Lindt, Bratek-Skicki et al. show that at carefully selected pH values, proteins can be kept in solution and their LLPS can then be induced by a jump to native pH. This presents a generic method to study the full kinetic trajectory of LLPS under near native conditions that can be easily controlle...

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Autores principales:	Joris Van Lindt, Anna Bratek-Skicki, Phuong N. Nguyen, Donya Pakravan, Luis F. Durán-Armenta, Agnes Tantos, Rita Pancsa, Ludo Van Den Bosch, Dominique Maes, Peter Tompa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/b7ebf01cce4d42dd989460bcbd0ea84b
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spelling	oai:doaj.org-article:b7ebf01cce4d42dd989460bcbd0ea84b2021-12-02T15:23:27ZA generic approach to study the kinetics of liquid–liquid phase separation under near-native conditions10.1038/s42003-020-01596-82399-3642https://doaj.org/article/b7ebf01cce4d42dd989460bcbd0ea84b2021-01-01T00:00:00Zhttps://doi.org/10.1038/s42003-020-01596-8https://doaj.org/toc/2399-3642Van Lindt, Bratek-Skicki et al. show that at carefully selected pH values, proteins can be kept in solution and their LLPS can then be induced by a jump to native pH. This presents a generic method to study the full kinetic trajectory of LLPS under near native conditions that can be easily controlled, providing a platform for the characterization of physiologically relevant phase-separation behaviour of diverse proteins.Joris Van LindtAnna Bratek-SkickiPhuong N. NguyenDonya PakravanLuis F. Durán-ArmentaAgnes TantosRita PancsaLudo Van Den BoschDominique MaesPeter TompaNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Joris Van Lindt Anna Bratek-Skicki Phuong N. Nguyen Donya Pakravan Luis F. Durán-Armenta Agnes Tantos Rita Pancsa Ludo Van Den Bosch Dominique Maes Peter Tompa A generic approach to study the kinetics of liquid–liquid phase separation under near-native conditions
description	Van Lindt, Bratek-Skicki et al. show that at carefully selected pH values, proteins can be kept in solution and their LLPS can then be induced by a jump to native pH. This presents a generic method to study the full kinetic trajectory of LLPS under near native conditions that can be easily controlled, providing a platform for the characterization of physiologically relevant phase-separation behaviour of diverse proteins.
format	article
author	Joris Van Lindt Anna Bratek-Skicki Phuong N. Nguyen Donya Pakravan Luis F. Durán-Armenta Agnes Tantos Rita Pancsa Ludo Van Den Bosch Dominique Maes Peter Tompa
author_facet	Joris Van Lindt Anna Bratek-Skicki Phuong N. Nguyen Donya Pakravan Luis F. Durán-Armenta Agnes Tantos Rita Pancsa Ludo Van Den Bosch Dominique Maes Peter Tompa
author_sort	Joris Van Lindt
title	A generic approach to study the kinetics of liquid–liquid phase separation under near-native conditions
title_short	A generic approach to study the kinetics of liquid–liquid phase separation under near-native conditions
title_full	A generic approach to study the kinetics of liquid–liquid phase separation under near-native conditions
title_fullStr	A generic approach to study the kinetics of liquid–liquid phase separation under near-native conditions
title_full_unstemmed	A generic approach to study the kinetics of liquid–liquid phase separation under near-native conditions
title_sort	generic approach to study the kinetics of liquid–liquid phase separation under near-native conditions
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b7ebf01cce4d42dd989460bcbd0ea84b
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A generic approach to study the kinetics of liquid–liquid phase separation under near-native conditions

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