Change in Cav3.2 T-Type Calcium Channel Induced by Varicella-Zoster Virus Participates in the Maintenance of Herpetic Neuralgia

Pain, as the most prevalent neurological complication of herpes zoster (HZ), may occur before or during the rash onset or even after the rash has recovered. Particularly, postherpetic neuralgia (PHN) is a refractory chronic condition, usually defined as pain persisting for 3 months or longer from th...

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Autores principales:	Rongzhen Li, Mingxi Ou, Shaomin Yang, Jiabin Huang, Jiamin Chen, Donglin Xiong, Lizu Xiao, Songbin Wu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Herpetic neuralgia T-type calcium channel Cav3.2 VZV dorsal root ganglion (DRG) spinal dorsal horn (SDH) Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/b7ed508f5f984f7aafb5c12e293f6f5f
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spelling	oai:doaj.org-article:b7ed508f5f984f7aafb5c12e293f6f5f2021-12-01T18:46:19ZChange in Cav3.2 T-Type Calcium Channel Induced by Varicella-Zoster Virus Participates in the Maintenance of Herpetic Neuralgia1664-229510.3389/fneur.2021.741054https://doaj.org/article/b7ed508f5f984f7aafb5c12e293f6f5f2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fneur.2021.741054/fullhttps://doaj.org/toc/1664-2295Pain, as the most prevalent neurological complication of herpes zoster (HZ), may occur before or during the rash onset or even after the rash has recovered. Particularly, postherpetic neuralgia (PHN) is a refractory chronic condition, usually defined as pain persisting for 3 months or longer from the onset of HZ. Pain evoked by HZ impairs the normal physical and emotional functions of the patients, severely reducing their quality of life. However, how zoster-associated pain occurs and develops into PHN are elusive, making PHN difficult to predict. Uncovering the pathogenesis of zoster-associated pain (or HN) helps us to better understand the onset of PHN and supports developing more effective treatments. In this study, we successfully constructed a model for zoster-associated pain through varicella-zoster virus (VZV) infections of mouse footpads and pain behavior assessments. Next, we used the Kyoto Encyclopedia of Genes and Genomes (KEGG) and the Gene Ontology (GO) to analyze PHN rodent dorsal root ganglion (DRG) gene microarray data and found that calcium signal disorder might be involved in the onset of PHN. By using reverse transcription real-time fluorescent quantitative PCR (RT-qPCR) and Western blotting, we confirmed that VZV infection could significantly upregulate the expression of T-type calcium channel Cav3.2 in DRG and spinal dorsal horn (SDH). Intrathecal administration of Cav3.2 blocker (2R/S)-6-prenylnaringenin (6-PNG) relieved mechanical and thermal hyperalgesia induced by VZV. Taken together, our data indicated that VZV might participate in the occurrence and development of HN by upregulating the expression of Cav3.2 in DRG and SDH. These findings will help to reveal the underlying mechanisms on long-lasting pain and PHN formation, providing a new insight that Cav3.2 can be the promising drug target for remitting PHN.Rongzhen LiMingxi OuShaomin YangJiabin HuangJiamin ChenDonglin XiongLizu XiaoSongbin WuFrontiers Media S.A.articleHerpetic neuralgiaT-type calcium channelCav3.2VZVdorsal root ganglion (DRG)spinal dorsal horn (SDH)Neurology. Diseases of the nervous systemRC346-429ENFrontiers in Neurology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Herpetic neuralgia T-type calcium channel Cav3.2 VZV dorsal root ganglion (DRG) spinal dorsal horn (SDH) Neurology. Diseases of the nervous system RC346-429
spellingShingle	Herpetic neuralgia T-type calcium channel Cav3.2 VZV dorsal root ganglion (DRG) spinal dorsal horn (SDH) Neurology. Diseases of the nervous system RC346-429 Rongzhen Li Mingxi Ou Shaomin Yang Jiabin Huang Jiamin Chen Donglin Xiong Lizu Xiao Songbin Wu Change in Cav3.2 T-Type Calcium Channel Induced by Varicella-Zoster Virus Participates in the Maintenance of Herpetic Neuralgia
description	Pain, as the most prevalent neurological complication of herpes zoster (HZ), may occur before or during the rash onset or even after the rash has recovered. Particularly, postherpetic neuralgia (PHN) is a refractory chronic condition, usually defined as pain persisting for 3 months or longer from the onset of HZ. Pain evoked by HZ impairs the normal physical and emotional functions of the patients, severely reducing their quality of life. However, how zoster-associated pain occurs and develops into PHN are elusive, making PHN difficult to predict. Uncovering the pathogenesis of zoster-associated pain (or HN) helps us to better understand the onset of PHN and supports developing more effective treatments. In this study, we successfully constructed a model for zoster-associated pain through varicella-zoster virus (VZV) infections of mouse footpads and pain behavior assessments. Next, we used the Kyoto Encyclopedia of Genes and Genomes (KEGG) and the Gene Ontology (GO) to analyze PHN rodent dorsal root ganglion (DRG) gene microarray data and found that calcium signal disorder might be involved in the onset of PHN. By using reverse transcription real-time fluorescent quantitative PCR (RT-qPCR) and Western blotting, we confirmed that VZV infection could significantly upregulate the expression of T-type calcium channel Cav3.2 in DRG and spinal dorsal horn (SDH). Intrathecal administration of Cav3.2 blocker (2R/S)-6-prenylnaringenin (6-PNG) relieved mechanical and thermal hyperalgesia induced by VZV. Taken together, our data indicated that VZV might participate in the occurrence and development of HN by upregulating the expression of Cav3.2 in DRG and SDH. These findings will help to reveal the underlying mechanisms on long-lasting pain and PHN formation, providing a new insight that Cav3.2 can be the promising drug target for remitting PHN.
format	article
author	Rongzhen Li Mingxi Ou Shaomin Yang Jiabin Huang Jiamin Chen Donglin Xiong Lizu Xiao Songbin Wu
author_facet	Rongzhen Li Mingxi Ou Shaomin Yang Jiabin Huang Jiamin Chen Donglin Xiong Lizu Xiao Songbin Wu
author_sort	Rongzhen Li
title	Change in Cav3.2 T-Type Calcium Channel Induced by Varicella-Zoster Virus Participates in the Maintenance of Herpetic Neuralgia
title_short	Change in Cav3.2 T-Type Calcium Channel Induced by Varicella-Zoster Virus Participates in the Maintenance of Herpetic Neuralgia
title_full	Change in Cav3.2 T-Type Calcium Channel Induced by Varicella-Zoster Virus Participates in the Maintenance of Herpetic Neuralgia
title_fullStr	Change in Cav3.2 T-Type Calcium Channel Induced by Varicella-Zoster Virus Participates in the Maintenance of Herpetic Neuralgia
title_full_unstemmed	Change in Cav3.2 T-Type Calcium Channel Induced by Varicella-Zoster Virus Participates in the Maintenance of Herpetic Neuralgia
title_sort	change in cav3.2 t-type calcium channel induced by varicella-zoster virus participates in the maintenance of herpetic neuralgia
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/b7ed508f5f984f7aafb5c12e293f6f5f
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Change in Cav3.2 T-Type Calcium Channel Induced by Varicella-Zoster Virus Participates in the Maintenance of Herpetic Neuralgia

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