Modeling of systemic inflammatory response syndrome by chemical induction of colon injury in rats

Our objective was to develop a model of systemic inflammatory response syndrome (SIRS) by chemical induction of colon injury and antibiotic-associated intestinal dysbiosis in rats with primary visceral obesity (PVO) for studies of myocardial resistance to ischemia-reperfusion injury. The experiments...

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Autores principales:	Yu. Yu. Borschev, I. Yu. Burovenko, A. B. Karaseva, S. M. Minasyan, V. Yu. Borschev, N. Yu. Semenova, O. V. Borshcheva, V. V. Polovinkin, G. G. Rodionov, A. N. Suvorov, M. M. Galagudza
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2020
Materias:	cytokines inflammatory colon disease antibiotic-induced dysbiosis isolated heart infarct size Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/b7f93259c7ce4f4698434544cdc32012
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spelling	oai:doaj.org-article:b7f93259c7ce4f4698434544cdc320122021-11-18T08:03:49ZModeling of systemic inflammatory response syndrome by chemical induction of colon injury in rats1563-06252313-741X10.15789/1563-0625-MOS-1839https://doaj.org/article/b7f93259c7ce4f4698434544cdc320122020-01-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1839https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XOur objective was to develop a model of systemic inflammatory response syndrome (SIRS) by chemical induction of colon injury and antibiotic-associated intestinal dysbiosis in rats with primary visceral obesity (PVO) for studies of myocardial resistance to ischemia-reperfusion injury. The experiments were performed with adult Wistar male rats with PVO under improved conditions of a conventional animal clinic. The chemically induced inflammatory colon disease (CIICD) was accomplished by intragastric administration of a mixture of broad-spectrum antimicrobial agents (AMA) for 3 days. Five days later, immunological and biochemical studies were carried out, as follows: composition of the intestinal microbiota in feces and shortchain fatty acids in blood, morphological changes in the structure of the colon, hemodynamic parameters and myocardial stability with modified Langendorff system. In PVO rats, the mass of visceral fat deposits and the content of lipopolysaccharides (LPS) in the blood were significantly increased when giving them fatcarbohydrate diet (FCD). In animals with CIICD, in addition to LPS, there was a significant increase in proinflammatory cytokine concentration (TNF, IL-8, MCP-1), and after oral administration of the AMA mixture, pronounced disturbances of food behavior and evacuatory function of gastrointestinal tract, deep destructive changes in colon, as well as qualitative and quantitative composition of intestinal microbiota with characteristics typical to the first-grade dysbiosis. High levels were shown for IL-8 cytokine only. An increase in acetic and propionic acid concentrations were shown in blood in animals with CIICD, and, to a greater extent, in rats with antibiotic-induced dysbiosis (AID). FCD was followed by significantly reduced levels of lactobacilli and bifidobacteria in colonic contents. CIICD leads to detection of Escherichia coli, and intestinal dysbiosis leads to the manifestation of Proteus. A comorbid combination of pathological changes in the immune and digestive systems caused a significant increase in the area of myocardial necrosis (by 35 percent) in isolated heart by, thus presuming decreased myocardial resistance to ischemia-reperfusion injury (IRI). The SIRS model induced by chemical trauma to large intestine is aggravated by the introduction of AMAs mixture, and it is characterized by a controlled change in inflammatory markers. Deterioration of morphofunctional characteristics in isolated heart included decrease in resistance to IRI seems to correspond to acute inflammatory bowel disease with induced intestinal dysbiosis. This model can be used in experimental medicine in the field of cardiology, endomicroecology, gastroenterology, and immunology.Yu. Yu. BorschevI. Yu. BurovenkoA. B. KarasevaS. M. MinasyanV. Yu. BorschevN. Yu. SemenovaO. V. BorshchevaV. V. PolovinkinG. G. RodionovA. N. SuvorovM. M. GalagudzaSPb RAACIarticlecytokinesinflammatory colon diseaseantibiotic-induced dysbiosisisolated heartinfarct sizeImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 22, Iss 1, Pp 87-98 (2020)
institution	DOAJ
collection	DOAJ
language	RU
topic	cytokines inflammatory colon disease antibiotic-induced dysbiosis isolated heart infarct size Immunologic diseases. Allergy RC581-607
spellingShingle	cytokines inflammatory colon disease antibiotic-induced dysbiosis isolated heart infarct size Immunologic diseases. Allergy RC581-607 Yu. Yu. Borschev I. Yu. Burovenko A. B. Karaseva S. M. Minasyan V. Yu. Borschev N. Yu. Semenova O. V. Borshcheva V. V. Polovinkin G. G. Rodionov A. N. Suvorov M. M. Galagudza Modeling of systemic inflammatory response syndrome by chemical induction of colon injury in rats
description	Our objective was to develop a model of systemic inflammatory response syndrome (SIRS) by chemical induction of colon injury and antibiotic-associated intestinal dysbiosis in rats with primary visceral obesity (PVO) for studies of myocardial resistance to ischemia-reperfusion injury. The experiments were performed with adult Wistar male rats with PVO under improved conditions of a conventional animal clinic. The chemically induced inflammatory colon disease (CIICD) was accomplished by intragastric administration of a mixture of broad-spectrum antimicrobial agents (AMA) for 3 days. Five days later, immunological and biochemical studies were carried out, as follows: composition of the intestinal microbiota in feces and shortchain fatty acids in blood, morphological changes in the structure of the colon, hemodynamic parameters and myocardial stability with modified Langendorff system. In PVO rats, the mass of visceral fat deposits and the content of lipopolysaccharides (LPS) in the blood were significantly increased when giving them fatcarbohydrate diet (FCD). In animals with CIICD, in addition to LPS, there was a significant increase in proinflammatory cytokine concentration (TNF, IL-8, MCP-1), and after oral administration of the AMA mixture, pronounced disturbances of food behavior and evacuatory function of gastrointestinal tract, deep destructive changes in colon, as well as qualitative and quantitative composition of intestinal microbiota with characteristics typical to the first-grade dysbiosis. High levels were shown for IL-8 cytokine only. An increase in acetic and propionic acid concentrations were shown in blood in animals with CIICD, and, to a greater extent, in rats with antibiotic-induced dysbiosis (AID). FCD was followed by significantly reduced levels of lactobacilli and bifidobacteria in colonic contents. CIICD leads to detection of Escherichia coli, and intestinal dysbiosis leads to the manifestation of Proteus. A comorbid combination of pathological changes in the immune and digestive systems caused a significant increase in the area of myocardial necrosis (by 35 percent) in isolated heart by, thus presuming decreased myocardial resistance to ischemia-reperfusion injury (IRI). The SIRS model induced by chemical trauma to large intestine is aggravated by the introduction of AMAs mixture, and it is characterized by a controlled change in inflammatory markers. Deterioration of morphofunctional characteristics in isolated heart included decrease in resistance to IRI seems to correspond to acute inflammatory bowel disease with induced intestinal dysbiosis. This model can be used in experimental medicine in the field of cardiology, endomicroecology, gastroenterology, and immunology.
format	article
author	Yu. Yu. Borschev I. Yu. Burovenko A. B. Karaseva S. M. Minasyan V. Yu. Borschev N. Yu. Semenova O. V. Borshcheva V. V. Polovinkin G. G. Rodionov A. N. Suvorov M. M. Galagudza
author_facet	Yu. Yu. Borschev I. Yu. Burovenko A. B. Karaseva S. M. Minasyan V. Yu. Borschev N. Yu. Semenova O. V. Borshcheva V. V. Polovinkin G. G. Rodionov A. N. Suvorov M. M. Galagudza
author_sort	Yu. Yu. Borschev
title	Modeling of systemic inflammatory response syndrome by chemical induction of colon injury in rats
title_short	Modeling of systemic inflammatory response syndrome by chemical induction of colon injury in rats
title_full	Modeling of systemic inflammatory response syndrome by chemical induction of colon injury in rats
title_fullStr	Modeling of systemic inflammatory response syndrome by chemical induction of colon injury in rats
title_full_unstemmed	Modeling of systemic inflammatory response syndrome by chemical induction of colon injury in rats
title_sort	modeling of systemic inflammatory response syndrome by chemical induction of colon injury in rats
publisher	SPb RAACI
publishDate	2020
url	https://doaj.org/article/b7f93259c7ce4f4698434544cdc32012
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Modeling of systemic inflammatory response syndrome by chemical induction of colon injury in rats

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