Pilot study on selective antimicrobial effect of a halitosis mouthrinse: monospecies and saliva-derived microbiome in an in vitro model system

Background Halitosis refers to malodor emanating from the oral cavity. Several mouthrinses with halitosis-reduction exist on the market, but their effect on the oral microbiome is largely unknown. In this study, we used an efficient in vitro model system to investigate a test mouthrinse's impac...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Márcia Botelho Dinis, Melissa Agnello, Xuesong He, Wenyuan Shi, Nini Chaichanasakul Tran
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	antimicrobial mouthrinse halitosis oral microbiome Infectious and parasitic diseases RC109-216 Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/b7fbcbf5b68e4dd09a1f71ec8da596c9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b7fbcbf5b68e4dd09a1f71ec8da596c9
record_format	dspace
spelling	oai:doaj.org-article:b7fbcbf5b68e4dd09a1f71ec8da596c92021-11-04T15:00:43ZPilot study on selective antimicrobial effect of a halitosis mouthrinse: monospecies and saliva-derived microbiome in an in vitro model system2000-229710.1080/20002297.2021.1996755https://doaj.org/article/b7fbcbf5b68e4dd09a1f71ec8da596c92021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/20002297.2021.1996755https://doaj.org/toc/2000-2297Background Halitosis refers to malodor emanating from the oral cavity. Several mouthrinses with halitosis-reduction exist on the market, but their effect on the oral microbiome is largely unknown. In this study, we used an efficient in vitro model system to investigate a test mouthrinse's impact on the oral microbiome. Methods Single halitosis-associated species and other common oral microorganism cultures were exposed to the test mouthrinse over time, and their viability was determined by culture-based selective plating. Next, the saliva-derived microbiome from healthy and halitosis-associated individuals was cultured in the presence of the test mouthrinse over time using the previously developed in vitro model system. The microbiome composition was assessed with 16S rRNA gene sequencing and downstream bioinformatics analyses. Results The test mouthrinse displayed antimicrobial activity against known anaerobic bacterial species producing halitosis-related compounds such as Fusobacterium nucleatum, F. periodonticum, and Prevotella intermedia but not against other common oral microorganisms. In the multispecies, saliva-derived cultures, mouthrinse exposure decreased the relative abundance of the Fusobacterium and Prevotella genera while not affecting overall diversity. Conclusions The test mouthrinse had promising anti-halitosis characteristics at the microbiome level, as demonstrated by the reduction in the relative abundance of halitosis-associated taxa while maintaining microbial diversity.Márcia Botelho DinisMelissa AgnelloXuesong HeWenyuan ShiNini Chaichanasakul TranTaylor & Francis Grouparticleantimicrobialmouthrinsehalitosisoral microbiomeInfectious and parasitic diseasesRC109-216MicrobiologyQR1-502ENJournal of Oral Microbiology, Vol 13, Iss 1 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antimicrobial mouthrinse halitosis oral microbiome Infectious and parasitic diseases RC109-216 Microbiology QR1-502
spellingShingle	antimicrobial mouthrinse halitosis oral microbiome Infectious and parasitic diseases RC109-216 Microbiology QR1-502 Márcia Botelho Dinis Melissa Agnello Xuesong He Wenyuan Shi Nini Chaichanasakul Tran Pilot study on selective antimicrobial effect of a halitosis mouthrinse: monospecies and saliva-derived microbiome in an in vitro model system
description	Background Halitosis refers to malodor emanating from the oral cavity. Several mouthrinses with halitosis-reduction exist on the market, but their effect on the oral microbiome is largely unknown. In this study, we used an efficient in vitro model system to investigate a test mouthrinse's impact on the oral microbiome. Methods Single halitosis-associated species and other common oral microorganism cultures were exposed to the test mouthrinse over time, and their viability was determined by culture-based selective plating. Next, the saliva-derived microbiome from healthy and halitosis-associated individuals was cultured in the presence of the test mouthrinse over time using the previously developed in vitro model system. The microbiome composition was assessed with 16S rRNA gene sequencing and downstream bioinformatics analyses. Results The test mouthrinse displayed antimicrobial activity against known anaerobic bacterial species producing halitosis-related compounds such as Fusobacterium nucleatum, F. periodonticum, and Prevotella intermedia but not against other common oral microorganisms. In the multispecies, saliva-derived cultures, mouthrinse exposure decreased the relative abundance of the Fusobacterium and Prevotella genera while not affecting overall diversity. Conclusions The test mouthrinse had promising anti-halitosis characteristics at the microbiome level, as demonstrated by the reduction in the relative abundance of halitosis-associated taxa while maintaining microbial diversity.
format	article
author	Márcia Botelho Dinis Melissa Agnello Xuesong He Wenyuan Shi Nini Chaichanasakul Tran
author_facet	Márcia Botelho Dinis Melissa Agnello Xuesong He Wenyuan Shi Nini Chaichanasakul Tran
author_sort	Márcia Botelho Dinis
title	Pilot study on selective antimicrobial effect of a halitosis mouthrinse: monospecies and saliva-derived microbiome in an in vitro model system
title_short	Pilot study on selective antimicrobial effect of a halitosis mouthrinse: monospecies and saliva-derived microbiome in an in vitro model system
title_full	Pilot study on selective antimicrobial effect of a halitosis mouthrinse: monospecies and saliva-derived microbiome in an in vitro model system
title_fullStr	Pilot study on selective antimicrobial effect of a halitosis mouthrinse: monospecies and saliva-derived microbiome in an in vitro model system
title_full_unstemmed	Pilot study on selective antimicrobial effect of a halitosis mouthrinse: monospecies and saliva-derived microbiome in an in vitro model system
title_sort	pilot study on selective antimicrobial effect of a halitosis mouthrinse: monospecies and saliva-derived microbiome in an in vitro model system
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/b7fbcbf5b68e4dd09a1f71ec8da596c9
work_keys_str_mv	AT marciabotelhodinis pilotstudyonselectiveantimicrobialeffectofahalitosismouthrinsemonospeciesandsalivaderivedmicrobiomeinaninvitromodelsystem AT melissaagnello pilotstudyonselectiveantimicrobialeffectofahalitosismouthrinsemonospeciesandsalivaderivedmicrobiomeinaninvitromodelsystem AT xuesonghe pilotstudyonselectiveantimicrobialeffectofahalitosismouthrinsemonospeciesandsalivaderivedmicrobiomeinaninvitromodelsystem AT wenyuanshi pilotstudyonselectiveantimicrobialeffectofahalitosismouthrinsemonospeciesandsalivaderivedmicrobiomeinaninvitromodelsystem AT ninichaichanasakultran pilotstudyonselectiveantimicrobialeffectofahalitosismouthrinsemonospeciesandsalivaderivedmicrobiomeinaninvitromodelsystem
_version_	1718444794488815616

Pilot study on selective antimicrobial effect of a halitosis mouthrinse: monospecies and saliva-derived microbiome in an in vitro model system

Ejemplares similares