Influence of Bioinspired Lithium-Doped Titanium Implants on Gingival Fibroblast Bioactivity and Biofilm Adhesion

Soft tissue integration (STI) at the transmucosal level around dental implants is crucial for the long-term success of dental implants. Surface modification of titanium dental implants could be an effective way to enhance peri-implant STI. The present study aimed to investigate the effect of bioinsp...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Aya Q. Alali, Abdalla Abdal-hay, Karan Gulati, Sašo Ivanovski, Benjamin P. J. Fournier, Ryan S. B. Lee
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	titanium implants nanostructure gingival fibroblasts biofilm soft-tissue integration Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/b7fd0f1a85bf46b4a8b564f86a3c00fd
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b7fd0f1a85bf46b4a8b564f86a3c00fd
record_format	dspace
spelling	oai:doaj.org-article:b7fd0f1a85bf46b4a8b564f86a3c00fd2021-11-25T18:29:53ZInfluence of Bioinspired Lithium-Doped Titanium Implants on Gingival Fibroblast Bioactivity and Biofilm Adhesion10.3390/nano111127992079-4991https://doaj.org/article/b7fd0f1a85bf46b4a8b564f86a3c00fd2021-10-01T00:00:00Zhttps://www.mdpi.com/2079-4991/11/11/2799https://doaj.org/toc/2079-4991Soft tissue integration (STI) at the transmucosal level around dental implants is crucial for the long-term success of dental implants. Surface modification of titanium dental implants could be an effective way to enhance peri-implant STI. The present study aimed to investigate the effect of bioinspired lithium (Li)-doped Ti surface on the behaviour of human gingival fibroblasts (HGFs) and oral biofilm <i>in vitro</i>. HGFs were cultured on various Ti surfaces—Li-doped Ti (Li_Ti), NaOH_Ti and micro-rough Ti (Control_Ti)—and were evaluated for viability, adhesion, extracellular matrix protein expression and cytokine secretion. Furthermore, single species bacteria (<i>Staphylococcus aureus</i>) and multi-species oral biofilms from saliva were cultured on each surface and assessed for viability and metabolic activity. The results show that both Li_Ti and NaOH_Ti significantly increased the proliferation of HGFs compared to the control. Fibroblast growth factor-2 (FGF-2) mRNA levels were significantly increased on Li_Ti and NaOH_Ti at day 7. Moreover, Li_Ti upregulated COL-I and fibronectin gene expression compared to the NaOH_Ti. A significant decrease in bacterial metabolic activity was detected for both the Li_Ti and NaOH_Ti surfaces. Together, these results suggest that bioinspired Li-doped Ti promotes HGF bioactivity while suppressing bacterial adhesion and growth. This is of clinical importance regarding STI improvement during the maintenance phase of the dental implant treatment.Aya Q. AlaliAbdalla Abdal-hayKaran GulatiSašo IvanovskiBenjamin P. J. FournierRyan S. B. LeeMDPI AGarticletitaniumimplantsnanostructuregingival fibroblastsbiofilmsoft-tissue integrationChemistryQD1-999ENNanomaterials, Vol 11, Iss 2799, p 2799 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	titanium implants nanostructure gingival fibroblasts biofilm soft-tissue integration Chemistry QD1-999
spellingShingle	titanium implants nanostructure gingival fibroblasts biofilm soft-tissue integration Chemistry QD1-999 Aya Q. Alali Abdalla Abdal-hay Karan Gulati Sašo Ivanovski Benjamin P. J. Fournier Ryan S. B. Lee Influence of Bioinspired Lithium-Doped Titanium Implants on Gingival Fibroblast Bioactivity and Biofilm Adhesion
description	Soft tissue integration (STI) at the transmucosal level around dental implants is crucial for the long-term success of dental implants. Surface modification of titanium dental implants could be an effective way to enhance peri-implant STI. The present study aimed to investigate the effect of bioinspired lithium (Li)-doped Ti surface on the behaviour of human gingival fibroblasts (HGFs) and oral biofilm <i>in vitro</i>. HGFs were cultured on various Ti surfaces—Li-doped Ti (Li_Ti), NaOH_Ti and micro-rough Ti (Control_Ti)—and were evaluated for viability, adhesion, extracellular matrix protein expression and cytokine secretion. Furthermore, single species bacteria (<i>Staphylococcus aureus</i>) and multi-species oral biofilms from saliva were cultured on each surface and assessed for viability and metabolic activity. The results show that both Li_Ti and NaOH_Ti significantly increased the proliferation of HGFs compared to the control. Fibroblast growth factor-2 (FGF-2) mRNA levels were significantly increased on Li_Ti and NaOH_Ti at day 7. Moreover, Li_Ti upregulated COL-I and fibronectin gene expression compared to the NaOH_Ti. A significant decrease in bacterial metabolic activity was detected for both the Li_Ti and NaOH_Ti surfaces. Together, these results suggest that bioinspired Li-doped Ti promotes HGF bioactivity while suppressing bacterial adhesion and growth. This is of clinical importance regarding STI improvement during the maintenance phase of the dental implant treatment.
format	article
author	Aya Q. Alali Abdalla Abdal-hay Karan Gulati Sašo Ivanovski Benjamin P. J. Fournier Ryan S. B. Lee
author_facet	Aya Q. Alali Abdalla Abdal-hay Karan Gulati Sašo Ivanovski Benjamin P. J. Fournier Ryan S. B. Lee
author_sort	Aya Q. Alali
title	Influence of Bioinspired Lithium-Doped Titanium Implants on Gingival Fibroblast Bioactivity and Biofilm Adhesion
title_short	Influence of Bioinspired Lithium-Doped Titanium Implants on Gingival Fibroblast Bioactivity and Biofilm Adhesion
title_full	Influence of Bioinspired Lithium-Doped Titanium Implants on Gingival Fibroblast Bioactivity and Biofilm Adhesion
title_fullStr	Influence of Bioinspired Lithium-Doped Titanium Implants on Gingival Fibroblast Bioactivity and Biofilm Adhesion
title_full_unstemmed	Influence of Bioinspired Lithium-Doped Titanium Implants on Gingival Fibroblast Bioactivity and Biofilm Adhesion
title_sort	influence of bioinspired lithium-doped titanium implants on gingival fibroblast bioactivity and biofilm adhesion
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b7fd0f1a85bf46b4a8b564f86a3c00fd
work_keys_str_mv	AT ayaqalali influenceofbioinspiredlithiumdopedtitaniumimplantsongingivalfibroblastbioactivityandbiofilmadhesion AT abdallaabdalhay influenceofbioinspiredlithiumdopedtitaniumimplantsongingivalfibroblastbioactivityandbiofilmadhesion AT karangulati influenceofbioinspiredlithiumdopedtitaniumimplantsongingivalfibroblastbioactivityandbiofilmadhesion AT sasoivanovski influenceofbioinspiredlithiumdopedtitaniumimplantsongingivalfibroblastbioactivityandbiofilmadhesion AT benjaminpjfournier influenceofbioinspiredlithiumdopedtitaniumimplantsongingivalfibroblastbioactivityandbiofilmadhesion AT ryansblee influenceofbioinspiredlithiumdopedtitaniumimplantsongingivalfibroblastbioactivityandbiofilmadhesion
_version_	1718411099781464064

Influence of Bioinspired Lithium-Doped Titanium Implants on Gingival Fibroblast Bioactivity and Biofilm Adhesion

Ejemplares similares