ASSOCIATION OF INTERLEUKIN-1α GENE POLYMORPHISM (ENCODED REGION rs1800587 5`UTR) WITH EFFICIENCY OF IN VITRO FERTILIZATION
Due to quite low efficiency and high costs of in vitro fertilization protocols, there is a necessity for development of new diagnostic criteria for selection of patients. The purpose of this study was complex analysis of association of polymorphic variants of IL-1α gene (rs1800587 5`UTR region) and...
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| Autores principales: | , , |
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| Formato: | article |
| Lenguaje: | RU |
| Publicado: |
SPb RAACI
2018
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/b80fb10b2bf3467489de3280d379062c |
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| Sumario: | Due to quite low efficiency and high costs of in vitro fertilization protocols, there is a necessity for development of new diagnostic criteria for selection of patients. The purpose of this study was complex analysis of association of polymorphic variants of IL-1α gene (rs1800587 5`UTR region) and main diagnostic criteria of women with tubo-peritoneal infertility and success rate of in vitro fertilization procedure. We have examined 120 women with tubo-peritoneal infertility who were subjected to the microflare protocol of controlled ovarian hyperstimulation which was followed by embryo transfer. Concentration of IL-1α in blood serum was defined by ELISA method by means of test systems of the "Cytokine LLC" (St. Petersburg, Russian Federation). Polymorphic marker rs1800587 at 5`UTR region was analysed with PCR method followed by the sequencing. We have detected no particular features in distribution of polymorphic IL-1α gene variants in pregnant women. The T/T genotype seems to be a conditionally favorable predictor for pregnancy resulting from in vitro fertilization. It has been shown that the IL-1α content in blood serum of pregnant women was increased as compared to women who did not become pregnant. It was also shown that pregnant women with T/T genotype had higher serum IL-1α levels than women with other genotypes. During transvaginal puncture, we have detected that pregnant women with T/T genotype had more oocytes than women with C/C genotype. We have not found significant differences in 17-oxyprogesterone, estradiol, follicle-stimulating hormone, testosterone contents for groups of women with different IL-1α genotypes. As based on this study, the IL-1a involvement in the implantation become more evident. Further studies are required concerning influence of IL-1a on folliculogenesis, ovulation, implantation, decidualization and placenta formation. |
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